Efficient Extraction of Astaxanthin from Phaffia rhodozyma with Polar and Non-polar Solvents after Acid Washing

doi:10.1016/S1004-9541(13)60510-8

Chinese Journal of Chemical Engineering ›› 2013, Vol. 21 ›› Issue (7): 776-780.DOI: 10.1016/S1004-9541(13)60510-8

Efficient Extraction of Astaxanthin from Phaffia rhodozyma with Polar and Non-polar Solvents after Acid Washing

尹春华, 杨淑珍, 刘晓璐, 闫海

Department of Biological Science and Engineering, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China

收稿日期:2012-03-03 修回日期:2012-12-10 出版日期:2013-07-28 发布日期:2013-08-24
通讯作者: YAN Hai
基金资助:
Supported by the Fundamental Research Funds for the Central Universities (FRF-AS-10-001B) and the National Natural Science Foundation of China (11071013).

Efficient Extraction of Astaxanthin from Phaffia rhodozyma with Polar and Non-polar Solvents after Acid Washing

YIN Chunhua, YANG Shuzhen, LIU Xiaolu, YAN Hai

Department of Biological Science and Engineering, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China

Received:2012-03-03 Revised:2012-12-10 Online:2013-07-28 Published:2013-08-24
Supported by:
Supported by the Fundamental Research Funds for the Central Universities (FRF-AS-10-001B) and the National Natural Science Foundation of China (11071013).

摘要/Abstract

摘要： method of extracting astaxanthin from Phaffia rhodozyma with various solvents after acid washing was investigated. The extraction efficiency was distinctly increased after acid washing of P. rhodozyma cells. When the concentration of HCl was 0.4 mol·L^-1, the highest extraction efficiency of astaxanthin was achieved which was about three times higher than the control. Acetone or benzene as single polar or non-polar solvent was the most effective solvent in our research. With a combination of isopropanol and n-hexane (volume ratio of 2:1), the maximal extraction efficiency was achieved, approximately 60% higher than that obtained with a single solvent. The liquid-solid ratio and the extracting time were also optimized. Under the optimum extraction conditions, the extraction yield of astaxanthin exceeded 98%.

关键词: astaxanthin, Phaffia rhodozyma, acid wash, extraction, solvent

Abstract: method of extracting astaxanthin from Phaffia rhodozyma with various solvents after acid washing was investigated. The extraction efficiency was distinctly increased after acid washing of P. rhodozyma cells. When the concentration of HCl was 0.4 mol·L^-1, the highest extraction efficiency of astaxanthin was achieved which was about three times higher than the control. Acetone or benzene as single polar or non-polar solvent was the most effective solvent in our research. With a combination of isopropanol and n-hexane (volume ratio of 2:1), the maximal extraction efficiency was achieved, approximately 60% higher than that obtained with a single solvent. The liquid-solid ratio and the extracting time were also optimized. Under the optimum extraction conditions, the extraction yield of astaxanthin exceeded 98%.

Key words: astaxanthin, Phaffia rhodozyma, acid wash, extraction, solvent

尹春华, 杨淑珍, 刘晓璐, 闫海. Efficient Extraction of Astaxanthin from Phaffia rhodozyma with Polar and Non-polar Solvents after Acid Washing[J]. Chinese Journal of Chemical Engineering, 2013, 21(7): 776-780.

YIN Chunhua, YANG Shuzhen, LIU Xiaolu, YAN Hai . Efficient Extraction of Astaxanthin from Phaffia rhodozyma with Polar and Non-polar Solvents after Acid Washing[J]. Chin.J.Chem.Eng., 2013, 21(7): 776-780.

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Efficient Extraction of Astaxanthin from Phaffia rhodozyma with Polar and Non-polar Solvents after Acid Washing

Efficient Extraction of Astaxanthin from Phaffia rhodozyma with Polar and Non-polar Solvents after Acid Washing

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