Nitrogen removal characteristics of heterotrophic nitrification-aerobic denitrification by Alcaligenes faecalis C16

doi:10.1016/j.cjche.2014.04.005

Chinese Journal of Chemical Engineering ›› 2015, Vol. 23 ›› Issue (5): 827-834.DOI: 10.1016/j.cjche.2014.04.005

Nitrogen removal characteristics of heterotrophic nitrification-aerobic denitrification by Alcaligenes faecalis C16

Yuxiang Liu¹, Yao Wang¹, Yi Li², Hua An¹, Yongkang Lv³

1 College of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, China;
2 National Major Science and Technology Program Management Office for Water Pollution Control and Treatment, Beijing 100029, China;
3 Shanxi Key Lab of Coal Science and Technology, Taiyuan University of Technology, Taiyuan 030024, China

收稿日期:2013-08-07 修回日期:2014-04-18 出版日期:2015-05-28 发布日期:2015-06-26
通讯作者: Yuxiang Liu
基金资助:
Supported by the National Natural Science Foundation of China (51078252), the Science and Technology Program of Taiyuan (120233), and the Natural Science Foundation of Shanxi Province (2010011016-1).

Nitrogen removal characteristics of heterotrophic nitrification-aerobic denitrification by Alcaligenes faecalis C16

Yuxiang Liu¹, Yao Wang¹, Yi Li², Hua An¹, Yongkang Lv³

1 College of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, China;
2 National Major Science and Technology Program Management Office for Water Pollution Control and Treatment, Beijing 100029, China;
3 Shanxi Key Lab of Coal Science and Technology, Taiyuan University of Technology, Taiyuan 030024, China

Received:2013-08-07 Revised:2014-04-18 Online:2015-05-28 Published:2015-06-26
Contact: Yuxiang Liu
Supported by:
Supported by the National Natural Science Foundation of China (51078252), the Science and Technology Program of Taiyuan (120233), and the Natural Science Foundation of Shanxi Province (2010011016-1).

摘要/Abstract

摘要： Alcaligenes faecalis C16 was found to have the ability to heterotrophically nitrify and aerobically denitrify. In order to further understand its nitrogen removal ability and mechanism, the growth and ammoniumremoval response were investigated at different C/N ratios and ammoniumconcentrations in the medium with citrate and acetate as carbon source separately. Furthermore, experiments of nitrogen sources, production of nitrogen gas and enzyme assay were conducted. Results show that the bacterium converts NH₄⁺-N and produces NH₂OH during the growing phase and nitrite accumulation is its distinct metabolic feature. A. faecalis C16 is able to tolerate not only high ammonium concentration but also high C/N ratio, and the ammonium tolerance is associated with carbon source and C/N ratio. The nitrogen balance under different conditions shows that approximately 28%-45% of the initial ammonium is assimilated into the cells, 44%-60% is denitrified and several percent is converted to nitrification products. A. faecalis C16 cannot utilize hydroxylamine, nitrite or nitrate as the sole nitrogen source for growth. However, nitrate can be used when ammonium is simultaneously present in the medium. A possible pathway for nitrogen removal by C16 is suggested. The preliminary enzyme assay provides more evidence for this nitrogen removal pathway.

关键词: Heterotrophic nitrification-aerobic, denitrification, Alcaligenes faecalis, Hydroxylamine oxidase, Nitrate reductase, Nitrite reductase

Abstract: Alcaligenes faecalis C16 was found to have the ability to heterotrophically nitrify and aerobically denitrify. In order to further understand its nitrogen removal ability and mechanism, the growth and ammoniumremoval response were investigated at different C/N ratios and ammoniumconcentrations in the medium with citrate and acetate as carbon source separately. Furthermore, experiments of nitrogen sources, production of nitrogen gas and enzyme assay were conducted. Results show that the bacterium converts NH₄⁺-N and produces NH₂OH during the growing phase and nitrite accumulation is its distinct metabolic feature. A. faecalis C16 is able to tolerate not only high ammonium concentration but also high C/N ratio, and the ammonium tolerance is associated with carbon source and C/N ratio. The nitrogen balance under different conditions shows that approximately 28%-45% of the initial ammonium is assimilated into the cells, 44%-60% is denitrified and several percent is converted to nitrification products. A. faecalis C16 cannot utilize hydroxylamine, nitrite or nitrate as the sole nitrogen source for growth. However, nitrate can be used when ammonium is simultaneously present in the medium. A possible pathway for nitrogen removal by C16 is suggested. The preliminary enzyme assay provides more evidence for this nitrogen removal pathway.

Key words: Heterotrophic nitrification-aerobic, denitrification, Alcaligenes faecalis, Hydroxylamine oxidase, Nitrate reductase, Nitrite reductase

Yuxiang Liu, Yao Wang, Yi Li, Hua An, Yongkang Lv. Nitrogen removal characteristics of heterotrophic nitrification-aerobic denitrification by Alcaligenes faecalis C16[J]. Chinese Journal of Chemical Engineering, 2015, 23(5): 827-834.

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Nitrogen removal characteristics of heterotrophic nitrification-aerobic denitrification by Alcaligenes faecalis C16

Nitrogen removal characteristics of heterotrophic nitrification-aerobic denitrification by Alcaligenes faecalis C16

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