SCI和EI收录∣中国化工学会会刊

Chinese Journal of Chemical Engineering ›› 2015, Vol. 23 ›› Issue (5): 835-841.DOI: 10.1016/j.cjche.2015.02.001

• 生物技术与生物工程 • 上一篇    下一篇

Simultaneous heterotrophic nitrification and aerobic denitrification at high initial phenol concentration by isolated bacterium Diaphorobacter sp. PD-7

Qilong Ge, Xiuping Yue, Guoying Wang   

  1. School of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, China
  • 收稿日期:2013-08-06 修回日期:2014-02-17 出版日期:2015-05-28 发布日期:2015-06-26
  • 通讯作者: Xiuping Yue
  • 基金资助:

    Supported by the National Natural Science Foundation of China (51378330 and 51408396), and the Natural Science Foundation of Shanxi Province (2013021023-3).

Simultaneous heterotrophic nitrification and aerobic denitrification at high initial phenol concentration by isolated bacterium Diaphorobacter sp. PD-7

Qilong Ge, Xiuping Yue, Guoying Wang   

  1. School of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, China
  • Received:2013-08-06 Revised:2014-02-17 Online:2015-05-28 Published:2015-06-26
  • Contact: Xiuping Yue
  • Supported by:

    Supported by the National Natural Science Foundation of China (51378330 and 51408396), and the Natural Science Foundation of Shanxi Province (2013021023-3).

摘要: A strain capable of phenol degradation, heterotrophic nitrification and aerobic denitrification was isolated from activated sludge of coking-plant waste water ponds under aerobic condition. Based on its morphology, physiology, biochemical analysis and phylogenetic characteristics, the isolate was identified as Diaphorobacter sp. PD-7. Biodegradation tests of phenol showed that the maximum phenol degradation occurred at the late phase of exponential growth stages, with 1400 mg·L-1 phenol completely degraded within 85 h. Diaphorobacter sp. PD-7 accumulated a vast quantity of phenol hydroxylase in this physiological phase, ensuring that the cells quickly utilize phenol as a sole carbon and energy source. The kinetic behavior of Diaphorobacter sp. PD-7 in batch cultures was investigated over a wide range of initial phenol concentrations (0-1400 mg·L-1) by using the Haldanemodel, which adequately describes the dynamic behavior of phenol biodegradation by strain Diaphorobacter sp. PD-7. At initial phenol concentration of 1400 mg·L-1, batch experiments (0.25 L flask) of nitrogen removal under aerobic condition gave almost entirely removal of 120.69 mg·L-1 ammonium nitrogen within 75 h, while nitrate nitrogen removal reached 91% within 65 h. Moreover, hydroxylamine oxidase, periplasmic nitrate reductase and nitrite reductase were successfully expressed in the isolate.

关键词: Diaphorobacter sp., Phenol biodegradation, Kinetics, Heterotrophic nitrification, Aerobic denitrification, Enzyme activity

Abstract: A strain capable of phenol degradation, heterotrophic nitrification and aerobic denitrification was isolated from activated sludge of coking-plant waste water ponds under aerobic condition. Based on its morphology, physiology, biochemical analysis and phylogenetic characteristics, the isolate was identified as Diaphorobacter sp. PD-7. Biodegradation tests of phenol showed that the maximum phenol degradation occurred at the late phase of exponential growth stages, with 1400 mg·L-1 phenol completely degraded within 85 h. Diaphorobacter sp. PD-7 accumulated a vast quantity of phenol hydroxylase in this physiological phase, ensuring that the cells quickly utilize phenol as a sole carbon and energy source. The kinetic behavior of Diaphorobacter sp. PD-7 in batch cultures was investigated over a wide range of initial phenol concentrations (0-1400 mg·L-1) by using the Haldanemodel, which adequately describes the dynamic behavior of phenol biodegradation by strain Diaphorobacter sp. PD-7. At initial phenol concentration of 1400 mg·L-1, batch experiments (0.25 L flask) of nitrogen removal under aerobic condition gave almost entirely removal of 120.69 mg·L-1 ammonium nitrogen within 75 h, while nitrate nitrogen removal reached 91% within 65 h. Moreover, hydroxylamine oxidase, periplasmic nitrate reductase and nitrite reductase were successfully expressed in the isolate.

Key words: Diaphorobacter sp., Phenol biodegradation, Kinetics, Heterotrophic nitrification, Aerobic denitrification, Enzyme activity