SCI和EI收录∣中国化工学会会刊

›› 2014, Vol. 22 ›› Issue (9): 1016-1020.DOI: 10.1016/j.cjche.2014.06.027

• 生物技术与生物工程 • 上一篇    下一篇

Sn-1,3-specific Interesterification of Soybean Oil with Medium-chain Triacylglycerol Catalyzed by Lipozyme TL IM

Hongli Yang1, YingMu1, Hongtao Chen1, Chunyang Su1, Tiankui Yang1,2, Zhilong Xiu1   

  1. 1 School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China;
    2 Wilmar (Shanghai) Biotechnology Research & Development Center Co., Ltd., Shanghai 200137, China
  • 收稿日期:2012-12-28 修回日期:2013-06-19 出版日期:2014-09-28 发布日期:2014-11-04
  • 通讯作者: Tiankui Yang, Zhilong Xiu
  • 基金资助:
    Supported by the National High Technology Research and Development Program of China (2010AA101505), and the National Natural Science Foundation of China (21106013).

Sn-1,3-specific Interesterification of Soybean Oil with Medium-chain Triacylglycerol Catalyzed by Lipozyme TL IM

Hongli Yang1, YingMu1, Hongtao Chen1, Chunyang Su1, Tiankui Yang1,2, Zhilong Xiu1   

  1. 1 School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China;
    2 Wilmar (Shanghai) Biotechnology Research & Development Center Co., Ltd., Shanghai 200137, China
  • Received:2012-12-28 Revised:2013-06-19 Online:2014-09-28 Published:2014-11-04
  • Supported by:
    Supported by the National High Technology Research and Development Program of China (2010AA101505), and the National Natural Science Foundation of China (21106013).

摘要: The structured lipids are produced through sn-1,3-specific interesterification of soybean oil with medium-chain triacylglycerol (MCT) in continuous reactions catalyzed by Thermomyces lanuginose lipase (Lipozyme TL IM). Cheap Lipozyme TL IM presents similar interesterification degree (ID), sn-1,3-specificity and residual activity as expensive Rhizomucor miehei in batch reactions. In packed-bed interesterification of soybean oil with MCT catalyzed by LipozymeTL IM, the residence time has a significant effect on ID,while temperature has a small effect at 45-70℃. The sn-1,3-specificity of Lipozyme TL IM is not satisfactory when reaction temperature is higher than 60℃. The optimal residence time and temperature are 30-40 min and 55℃, respectively. Among the solvents, including acetone, isopropanol, tert-butanol, and isobutanol, used to recover the activity of the used Lipozyme TL IM, acetone is the most suitable one than the other solvents.

关键词: Sn-1,3-specificity, Interesterification, Thermomyces lanuginose lipase, Medium-chain triacylglycerol, Structured lipids

Abstract: The structured lipids are produced through sn-1,3-specific interesterification of soybean oil with medium-chain triacylglycerol (MCT) in continuous reactions catalyzed by Thermomyces lanuginose lipase (Lipozyme TL IM). Cheap Lipozyme TL IM presents similar interesterification degree (ID), sn-1,3-specificity and residual activity as expensive Rhizomucor miehei in batch reactions. In packed-bed interesterification of soybean oil with MCT catalyzed by LipozymeTL IM, the residence time has a significant effect on ID,while temperature has a small effect at 45-70℃. The sn-1,3-specificity of Lipozyme TL IM is not satisfactory when reaction temperature is higher than 60℃. The optimal residence time and temperature are 30-40 min and 55℃, respectively. Among the solvents, including acetone, isopropanol, tert-butanol, and isobutanol, used to recover the activity of the used Lipozyme TL IM, acetone is the most suitable one than the other solvents.

Key words: Sn-1,3-specificity, Interesterification, Thermomyces lanuginose lipase, Medium-chain triacylglycerol, Structured lipids