A strong adhesive block polymer coating for antifouling of large molecular weight protein

doi:10.1016/j.cjche.2017.04.007

摘要/Abstract

摘要： Some proteins secreted by microorganisms have large molecularweights.Wereport here an approach to prepare coating by multilayer polymers for antifouling of proteins, especially the proteins with a large molecular weight. Stainless steel was used as the model substrate. The substrate was first coatedwith a hybrid polymer film, which was formed by simultaneous hydrolytic polycondensation of 3-aminopropyltriethoxysilane and polymerization of dopamine (HPAPD). After grafting the macroinitiator 2-bromoisobutyryl bromide, the block polymer brushes PMMA-b-PHEMA were grafted. Three proteins were used to test protein adsorption and antifouling behavior of the coating, including recombinant green fluorescent (54 kDa), recombinant R-transaminase (2×90 kDa), and recombinant catalase (4×98 kDa). It is demonstrated that the block polymer brushes not only can prevent the adsorption of small molecular weight proteins, but also can significantly reduce the adsorption of the large molecular weight proteins.

关键词: Antifouling, Protein, Block polymer, Surface, Polymerization

Abstract: Some proteins secreted by microorganisms have large molecularweights.Wereport here an approach to prepare coating by multilayer polymers for antifouling of proteins, especially the proteins with a large molecular weight. Stainless steel was used as the model substrate. The substrate was first coatedwith a hybrid polymer film, which was formed by simultaneous hydrolytic polycondensation of 3-aminopropyltriethoxysilane and polymerization of dopamine (HPAPD). After grafting the macroinitiator 2-bromoisobutyryl bromide, the block polymer brushes PMMA-b-PHEMA were grafted. Three proteins were used to test protein adsorption and antifouling behavior of the coating, including recombinant green fluorescent (54 kDa), recombinant R-transaminase (2×90 kDa), and recombinant catalase (4×98 kDa). It is demonstrated that the block polymer brushes not only can prevent the adsorption of small molecular weight proteins, but also can significantly reduce the adsorption of the large molecular weight proteins.

Key words: Antifouling, Protein, Block polymer, Surface, Polymerization

Xianling Meng, Xia Jiang, Peijun Ji. A strong adhesive block polymer coating for antifouling of large molecular weight protein[J]. Chinese Journal of Chemical Engineering, 2017, 25(12): 1831-1837.

Xianling Meng, Xia Jiang, Peijun Ji. A strong adhesive block polymer coating for antifouling of large molecular weight protein[J]. Chin.J.Chem.Eng., 2017, 25(12): 1831-1837.

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