SCI和EI收录∣中国化工学会会刊

Chinese Journal of Chemical Engineering ›› 2018, Vol. 26 ›› Issue (7): 1586-1590.DOI: 10.1016/j.cjche.2018.01.026

• Materials and Product Engineering • 上一篇    下一篇

Preparation of vapreotide-templated silver nanocages and their photothermal therapy efficacy

Ruiyan Zhu1,2, Yanji Li1, Kexin Bian1, Zhengrong Gao3, Dawei Gao1   

  1. 1 Applied Chemistry Key Lab of Hebei Province, Department of Bioengineering, Yanshan University, Qinhuangdao 066004, China;
    2 Hebei Province Asparagus Industry Technology Research Institute, Qinhuangdao 066004, China;
    3 Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China
  • 收稿日期:2017-10-23 修回日期:2017-12-26 出版日期:2018-07-28 发布日期:2018-08-16
  • 通讯作者: Dawei Gao,E-mail address:dwgao@ysu.edu.cn
  • 基金资助:

    Supported by the National Natural Science Foundation of China (21476190) and Hebei Province Key Basic Research Fund (15961301D).

Preparation of vapreotide-templated silver nanocages and their photothermal therapy efficacy

Ruiyan Zhu1,2, Yanji Li1, Kexin Bian1, Zhengrong Gao3, Dawei Gao1   

  1. 1 Applied Chemistry Key Lab of Hebei Province, Department of Bioengineering, Yanshan University, Qinhuangdao 066004, China;
    2 Hebei Province Asparagus Industry Technology Research Institute, Qinhuangdao 066004, China;
    3 Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China
  • Received:2017-10-23 Revised:2017-12-26 Online:2018-07-28 Published:2018-08-16
  • Contact: Dawei Gao,E-mail address:dwgao@ysu.edu.cn
  • Supported by:

    Supported by the National Natural Science Foundation of China (21476190) and Hebei Province Key Basic Research Fund (15961301D).

摘要: Vapreotide acetate (Vap) was used as a biotemplate to synthesize silver nanocages through direct co-incubation of a AgNO3 solution, following by reduction using fresh NaBH4. The characterized vapreotide-templated silver nanocages (Vap-AgNCs) presented a wide and red shifted absorption band with a maximum between 480 nm and 800 nm and possessed a uniform structure with a face-centered cubic crystal structure. The biocompatibility of Vap-AgNCs was assessed using the MTT method, indicating Vap-AgNCs had better biocompatibility when its concentration was lower than 2.5×10-4 mmol·L-1. The photothermal characteristics of Vap-AgNCs were analyzed with laser irradiation (808 nm, 1.5 W·cm-2) and the results showed that the temperature of the VapAgNCs solution reached 45℃ starting from 25℃ within 5 min. Additionally, Vap-AgNCs with a laser led to HeLa cell death. Therefore, the prepared Vap-AgNCs is expected to be an effective photothermal therapy agent.

关键词: Silver nanocages, Vapreotide acetate, Biotemplate, Photothermal therapy, Nanoparticles

Abstract: Vapreotide acetate (Vap) was used as a biotemplate to synthesize silver nanocages through direct co-incubation of a AgNO3 solution, following by reduction using fresh NaBH4. The characterized vapreotide-templated silver nanocages (Vap-AgNCs) presented a wide and red shifted absorption band with a maximum between 480 nm and 800 nm and possessed a uniform structure with a face-centered cubic crystal structure. The biocompatibility of Vap-AgNCs was assessed using the MTT method, indicating Vap-AgNCs had better biocompatibility when its concentration was lower than 2.5×10-4 mmol·L-1. The photothermal characteristics of Vap-AgNCs were analyzed with laser irradiation (808 nm, 1.5 W·cm-2) and the results showed that the temperature of the VapAgNCs solution reached 45℃ starting from 25℃ within 5 min. Additionally, Vap-AgNCs with a laser led to HeLa cell death. Therefore, the prepared Vap-AgNCs is expected to be an effective photothermal therapy agent.

Key words: Silver nanocages, Vapreotide acetate, Biotemplate, Photothermal therapy, Nanoparticles