Lysozyme adsorption to cation exchanger derivatized by sequential modification of poly(ethylenimine)-Sepharose with succinic anhydride and ethanolamine: Effect of pH and ionic strength

doi:10.1016/j.cjche.2019.07.003

中国化学工程学报 ›› 2020, Vol. 28 ›› Issue (2): 440-444.DOI: 10.1016/j.cjche.2019.07.003

• Separation Science and Engineering • 上一篇下一篇

Lysozyme adsorption to cation exchanger derivatized by sequential modification of poly(ethylenimine)-Sepharose with succinic anhydride and ethanolamine: Effect of pH and ionic strength

Yangyang Zhao, Xianxiu Li, Linling Yu, Xiaoyan Dong, Yan Sun

Department of Biochemical Engineering and Key Laboratory of Systems Bioengineering of the Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300354, China

收稿日期:2019-04-12 修回日期:2019-06-18 出版日期:2020-02-28 发布日期:2020-05-21
通讯作者: Xiaoyan Dong
基金资助:
This work was supported by the National Natural Science Foundation of China (Nos. 21878222 and 21621004).

Lysozyme adsorption to cation exchanger derivatized by sequential modification of poly(ethylenimine)-Sepharose with succinic anhydride and ethanolamine: Effect of pH and ionic strength

Yangyang Zhao, Xianxiu Li, Linling Yu, Xiaoyan Dong, Yan Sun

Department of Biochemical Engineering and Key Laboratory of Systems Bioengineering of the Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300354, China

Received:2019-04-12 Revised:2019-06-18 Online:2020-02-28 Published:2020-05-21
Contact: Xiaoyan Dong
Supported by:
This work was supported by the National Natural Science Foundation of China (Nos. 21878222 and 21621004).

摘要/Abstract

摘要： In our previous work, a series of polyethylenimine (PEI)-derived cation exchangers were synthesized using PEIgrafted resin FF-PEI-L740 (ionic capacity, 740 mmol·L^-1) as the basic resin to study lysozyme adsorption and chromatographic behavior. It was found that the resin with an ionic capacity of 630 mmol·L^-1 (FF-PEI-CR630) possessed high adsorption performance towards lysozyme at 0-100 mmol·L^-1 NaCl. Therefore, in this work, FF-PEI-CR630 was selected to study the influences of pH and ionic strength (IS) on protein adsorption and chromatographic behavior towards lysozyme. The increase of lysozyme adsorption capacity in the pH range of 6 to 10 was observed. However, the uptake rate decreased in the pH range of 6 to 8 and then remained essentially unchanged from pH 8 to pH 10. Increasing IS led to decreased protein adsorption capacity and increased uptake rate in different pH ranges. Besides, FF-PEI-CR630 maintained dynamic binding capacity as high as over 150 mg·ml^-1 at pH 8-10 without NaCl. The research has thus provided insight into the selection of proper pH and IS conditions for protein purification by using FF-PEI-CR630.

关键词: Ion exchange, pH value, Adsorption, Kinetics, Chain delivery, Dynamic binding capacity

Abstract: In our previous work, a series of polyethylenimine (PEI)-derived cation exchangers were synthesized using PEIgrafted resin FF-PEI-L740 (ionic capacity, 740 mmol·L^-1) as the basic resin to study lysozyme adsorption and chromatographic behavior. It was found that the resin with an ionic capacity of 630 mmol·L^-1 (FF-PEI-CR630) possessed high adsorption performance towards lysozyme at 0-100 mmol·L^-1 NaCl. Therefore, in this work, FF-PEI-CR630 was selected to study the influences of pH and ionic strength (IS) on protein adsorption and chromatographic behavior towards lysozyme. The increase of lysozyme adsorption capacity in the pH range of 6 to 10 was observed. However, the uptake rate decreased in the pH range of 6 to 8 and then remained essentially unchanged from pH 8 to pH 10. Increasing IS led to decreased protein adsorption capacity and increased uptake rate in different pH ranges. Besides, FF-PEI-CR630 maintained dynamic binding capacity as high as over 150 mg·ml^-1 at pH 8-10 without NaCl. The research has thus provided insight into the selection of proper pH and IS conditions for protein purification by using FF-PEI-CR630.

Key words: Ion exchange, pH value, Adsorption, Kinetics, Chain delivery, Dynamic binding capacity

Yangyang Zhao, Xianxiu Li, Linling Yu, Xiaoyan Dong, Yan Sun. Lysozyme adsorption to cation exchanger derivatized by sequential modification of poly(ethylenimine)-Sepharose with succinic anhydride and ethanolamine: Effect of pH and ionic strength[J]. 中国化学工程学报, 2020, 28(2): 440-444.

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Lysozyme adsorption to cation exchanger derivatized by sequential modification of poly(ethylenimine)-Sepharose with succinic anhydride and ethanolamine: Effect of pH and ionic strength

Lysozyme adsorption to cation exchanger derivatized by sequential modification of poly(ethylenimine)-Sepharose with succinic anhydride and ethanolamine: Effect of pH and ionic strength

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