Split internal-loop photobioreactor for Scenedesmus sp. microalgae: Culturing and hydrodynamics

doi:10.1016/j.cjche.2020.07.058

中国化学工程学报 ›› 2021, Vol. 33 ›› Issue (5): 236-248.DOI: 10.1016/j.cjche.2020.07.058

• Biotechnology and Bioengineering • 上一篇下一篇

Split internal-loop photobioreactor for Scenedesmus sp. microalgae: Culturing and hydrodynamics

Laith S. Sabri^1,2, Abbas J. Sultan^1,2, Muthanna H. Al-Dahhan¹

1 Multiphase Flow and Reactors Engineering and Application Laboratory (mReal), Chemical and Biochemical Engineering Department, Missouri University of Science and Technology, Rolla, MO;
65409-1230. USA;
2 University of Technology, Chemical Engineering Department, Baghdad, Iraq

收稿日期:2020-03-25 修回日期:2020-07-18 出版日期:2021-05-28 发布日期:2021-08-19
通讯作者: Laith S. Sabri
基金资助:
The authors would like to acknowledge the financial aid provided by the Iraqi government, the Ministry of Higher Education Iraq, and the Higher Committee for Education Development in Iraq (HCED) and the fund provided by Missouri S&T.

Split internal-loop photobioreactor for Scenedesmus sp. microalgae: Culturing and hydrodynamics

Laith S. Sabri^1,2, Abbas J. Sultan^1,2, Muthanna H. Al-Dahhan¹

1 Multiphase Flow and Reactors Engineering and Application Laboratory (mReal), Chemical and Biochemical Engineering Department, Missouri University of Science and Technology, Rolla, MO;
65409-1230. USA;
2 University of Technology, Chemical Engineering Department, Baghdad, Iraq

Received:2020-03-25 Revised:2020-07-18 Online:2021-05-28 Published:2021-08-19
Contact: Laith S. Sabri
Supported by:
The authors would like to acknowledge the financial aid provided by the Iraqi government, the Ministry of Higher Education Iraq, and the Higher Committee for Education Development in Iraq (HCED) and the fund provided by Missouri S&T.

摘要/Abstract

摘要： In this study, the evaluation of the performance of the split internal loop photobioreactor for culturing a species of green microalgae, Scenedesmus sp. under different operating superficial gas velocity and during a different time of growth (i.e., starting for the first day until end day of the culturing process) was addressed. The evaluation of the performance of the split internal loop photobioreactor was included assessing the density, pH, temperature, viscosity, surface tension, the optical density, cell population, dry biomass, and chlorophyll of the culture medium of the microalgae culturing. Additionally, the hydrodynamics of a Split Internal-Loop Photobioreactor with microalgae culturing was comprehensively quantified. Radioactive particle tracking (RPT) and gamma-ray computed tomography (CT) techniques were applied for the first time to quantify and address the influence of microalgae culture on the hydrodynamic parameters. The hydrodynamics parameters such as local liquid velocity field, shear stresses, turbulent kinetic energy, and local gas holdup profiles were measured at different superficial gas velocities as well as under different times of algae growth. The obtained results indicate that the flow distribution may significantly affect the performance of the photobioreactor, which may have substantial effects on the cultivation process. The obtained experimental data can serve as benchmark data for the evaluation and validation of computational fluid dynamics (CFD) codes and their closures. This, in turn, allows us to develop efficient reactors and consequently improving the productivity and selectivity of these photobioreactors.

关键词: Scenedesmus microalgae, Cylindrical airlift photobioreactor, Physical properties, Non-invasive gamma-ray technique

Abstract: In this study, the evaluation of the performance of the split internal loop photobioreactor for culturing a species of green microalgae, Scenedesmus sp. under different operating superficial gas velocity and during a different time of growth (i.e., starting for the first day until end day of the culturing process) was addressed. The evaluation of the performance of the split internal loop photobioreactor was included assessing the density, pH, temperature, viscosity, surface tension, the optical density, cell population, dry biomass, and chlorophyll of the culture medium of the microalgae culturing. Additionally, the hydrodynamics of a Split Internal-Loop Photobioreactor with microalgae culturing was comprehensively quantified. Radioactive particle tracking (RPT) and gamma-ray computed tomography (CT) techniques were applied for the first time to quantify and address the influence of microalgae culture on the hydrodynamic parameters. The hydrodynamics parameters such as local liquid velocity field, shear stresses, turbulent kinetic energy, and local gas holdup profiles were measured at different superficial gas velocities as well as under different times of algae growth. The obtained results indicate that the flow distribution may significantly affect the performance of the photobioreactor, which may have substantial effects on the cultivation process. The obtained experimental data can serve as benchmark data for the evaluation and validation of computational fluid dynamics (CFD) codes and their closures. This, in turn, allows us to develop efficient reactors and consequently improving the productivity and selectivity of these photobioreactors.

Key words: Scenedesmus microalgae, Cylindrical airlift photobioreactor, Physical properties, Non-invasive gamma-ray technique

Laith S. Sabri, Abbas J. Sultan, Muthanna H. Al-Dahhan. Split internal-loop photobioreactor for Scenedesmus sp. microalgae: Culturing and hydrodynamics[J]. 中国化学工程学报, 2021, 33(5): 236-248.

Laith S. Sabri, Abbas J. Sultan, Muthanna H. Al-Dahhan. Split internal-loop photobioreactor for Scenedesmus sp. microalgae: Culturing and hydrodynamics[J]. Chinese Journal of Chemical Engineering, 2021, 33(5): 236-248.

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Split internal-loop photobioreactor for Scenedesmus sp. microalgae: Culturing and hydrodynamics

Split internal-loop photobioreactor for Scenedesmus sp. microalgae: Culturing and hydrodynamics

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