Production and characterization of insoluble α-1,3-linked glucan and soluble α-1,6-linked dextran from Leuconostoc pseudomesenteroides G29

doi:10.1016/j.cjche.2021.06.020

中国化学工程学报 ›› 2021, Vol. 39 ›› Issue (11): 211-218.DOI: 10.1016/j.cjche.2021.06.020

• Biotechnology and Bioengineering • 上一篇下一篇

Production and characterization of insoluble α-1,3-linked glucan and soluble α-1,6-linked dextran from Leuconostoc pseudomesenteroides G29

Yiya Wang, Tao Sun, Yinzhu Wang, Hao Wu, Yan Fang, Jiangfeng Ma, Min Jiang

State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, China

收稿日期:2021-02-22 修回日期:2021-06-22 出版日期:2021-11-28 发布日期:2021-12-27
通讯作者: Jiangfeng Ma
基金资助:
This work was supported by Jiangsu Key Lab of Biomass-based Green Fuels and Chemicals Foundation (JSBEM2016010), Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture of China.

Production and characterization of insoluble α-1,3-linked glucan and soluble α-1,6-linked dextran from Leuconostoc pseudomesenteroides G29

Yiya Wang, Tao Sun, Yinzhu Wang, Hao Wu, Yan Fang, Jiangfeng Ma, Min Jiang

State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, China

Received:2021-02-22 Revised:2021-06-22 Online:2021-11-28 Published:2021-12-27
Contact: Jiangfeng Ma
Supported by:
This work was supported by Jiangsu Key Lab of Biomass-based Green Fuels and Chemicals Foundation (JSBEM2016010), Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture of China.

摘要/Abstract

摘要： Exopolysaccharides can be produced by various bacteria and have important biological roles in bacterial survival depend on molecular weight, linkage, and conformation. In this study, Leuconostoc pseudomesenteroides G29 was identified and found to produce two types of exopolysaccharides from sucrose including soluble and insoluble α-glucans. By regulation of pH above 5.5, soluble α-glucan production was increased to 38.4 g·L^-1 from 101.4 g·L^-1 sucrose with fewer accumulation of lactic acid and acetic acid. Simultaneously, the quantity of thick white precipitate, that is insoluble α-glucan, was also increased. Then, α-glucans were prepared by enzymatic reaction with crude glucansucrases from the supernatant of G29 fermentation broth and purified for structure analysis. Based on the integration analysis of FT-IR and NMR, it was observed that soluble α-glucan is a highly linear dextran with α-1,6 glycosidic bonds while the insoluble α-glucan has 93% of α-1,3 and 7% of α-1,6 glycosidic bond. The results extend our understanding of exopolysaccharides production by L. pseudomesenteroides, and this water insoluble α-1,3-glucan might have potential application as biomaterials and/or biochemicals.

关键词: Exopolysaccharide, Glucansucrase, Leuconostoc pseudomesenteroides, Insoluble α-glucan, α-1,3 glycosidic bond

Abstract: Exopolysaccharides can be produced by various bacteria and have important biological roles in bacterial survival depend on molecular weight, linkage, and conformation. In this study, Leuconostoc pseudomesenteroides G29 was identified and found to produce two types of exopolysaccharides from sucrose including soluble and insoluble α-glucans. By regulation of pH above 5.5, soluble α-glucan production was increased to 38.4 g·L^-1 from 101.4 g·L^-1 sucrose with fewer accumulation of lactic acid and acetic acid. Simultaneously, the quantity of thick white precipitate, that is insoluble α-glucan, was also increased. Then, α-glucans were prepared by enzymatic reaction with crude glucansucrases from the supernatant of G29 fermentation broth and purified for structure analysis. Based on the integration analysis of FT-IR and NMR, it was observed that soluble α-glucan is a highly linear dextran with α-1,6 glycosidic bonds while the insoluble α-glucan has 93% of α-1,3 and 7% of α-1,6 glycosidic bond. The results extend our understanding of exopolysaccharides production by L. pseudomesenteroides, and this water insoluble α-1,3-glucan might have potential application as biomaterials and/or biochemicals.

Key words: Exopolysaccharide, Glucansucrase, Leuconostoc pseudomesenteroides, Insoluble α-glucan, α-1,3 glycosidic bond

Yiya Wang, Tao Sun, Yinzhu Wang, Hao Wu, Yan Fang, Jiangfeng Ma, Min Jiang. Production and characterization of insoluble α-1,3-linked glucan and soluble α-1,6-linked dextran from Leuconostoc pseudomesenteroides G29[J]. 中国化学工程学报, 2021, 39(11): 211-218.

参考文献

[1] K. Wangpaiboon, N. Waiyaseesang, P. Panpetch, T. Charoenwongpaiboon, S.A. Nepogodiev, S. Ekgasit, R.A. Field, R. Pichayangkura, Characterisation of insoluble a-1,3-/a-1,6 mixed linkage glucan produced in addition to soluble a-1,6-linked dextran by glucansucrase (DEX-N) from Leuconostoc citreum ABK- 1, Int. J. Biol. Macromol. 152(2020) 473-482.
[2] A. Synytsya, M. Novák, Structural diversity of fungal glucans, Carbohydr. Polym. 92(1) (2013) 792-809.
[3] J. Gangoiti, T. Pijning, L. Dijkhuizen, Biotechnological potential of novel glycoside hydrolase family 70 enzymes synthesizing a-glucans from starch and sucrose, Biotechnol. Adv. 36(1) (2018) 196-207.
[4] B. Wang, Q. Song, F. Zhao, H. Xiao, Z. Zhou, Y.e. Han, Purification and characterization of dextran produced by Leuconostoc pseudomesenteroides PC as a potential exopolysaccharide suitable for food applications, Process Biochem. 87(2019) 187-195.
[5] K. Złotko, A. Wiater, A. Waśko, M. Pleszczyńska, R. Paduch, J. Jaroszuk-Ściseł, A. Bieganowski, A report on fungal (1→3)-α-d-glucans:properties, functions and application, Molecules 24(2019) 3972.
[6] M. Malten, R. Hollmann, W.-D. Deckwer, D. Jahn, Production and secretion of recombinant Leuconostoc mesenteroides dextransucrase DsrS in Bacillus megaterium, Biotechnol. Bioeng. 89(2) (2005) 206-218.
[7] P.M. Coutinho, E. Deleury, G.J. Davies, B. Henrissat, An evolving hierarchical family classification for glycosyltransferases, J. Mol. Biol. 328(2) (2003) 307- 317.
[8] T.Q. Wang, H.X. Li, K.L. Nie, T.W. Tan, Immobilization of lipase on epoxy activated (1→3)-α-d-glucan isolated from Penicillium chrysongenum, Biosci. Biotechnol. Biochem. 70(2006) 2883-2888.
[9] A. Wiater, R. Paduch, M. Pleszczyńska, K. Próchniak, A. Choma, M. KandeferSzerszeń, J. Szczodrak, α-(1→3)-d-Glucans from fruiting bodies of selected macromycetes fungi and the biological activity of their carboxymethylated products, Biotechnol. Lett. 33(2011) 787-795.
[10] F. Yangilar, P.O. Yildiz, Microbial polysaccharides and the applications in food industry, J. Biotechnol. 231(2016) S38.
[11] F.R. Seymour, R.L. Julian, A. Jeanes, B.L. Lamberts, Structural analysis of insoluble d-glucans by fourier-transform, infrared difference-spectrometry:correlation between structures of dextrans from strains of leuconostoc mesenteroides and of d-glucans from strains of streptococcus mutans, Carbohydr. Res. 86(1980) 227-246.
[12] G.L. Côté, C.D. Skory, Cloning, expression, and characterization of an insoluble glucan-producing glucansucrase from Leuconostoc mesenteroides NRRL B-1118, Appl. Microbiol. Biotechnol. 93(6) (2012) 2387-2394.
[13] Z. Chen, D. Ni, W. Zhang, T. Stressler, W. Mu, Lactic acid bacteria-derived aglucans:From enzymatic synthesis to miscellaneous applications, Biotechnol. Adv. 47(2021) 107708.
[14] M. Miao, A. Bai, B.o. Jiang, Y. Song, S.W. Cui, T. Zhang, Characterisation of a novel water-soluble polysaccharide from Leuconostoc citreum SK24.002, Food Hydrocoll. 36(2014) 265-272.
[15] X.G. Li, Z.K. Wang, F.J. Lu, H.M. Zhang, J.W. Tian, L.L. He, Y.W. Chu, Y.Q. Tian, Actinocorallia populi sp. nov., an endophytic actinomycete isolated from a root of Populus adenopoda (Maxim.), Int. J. Syst. Evol. Microbiol. 68(2018) 2325- 2330.
[16] D.H. Huson, D. Bryant, Application of phylogenetic networks in evolutionary studies, Mol. Biol. Evol. 23(2006) 254-267.
[17] K. Tamura, D. Peterson, N. Peterson, G. Stecher, M. Nei, S. Kumar, MEGA5:Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods, Mol. Biol. Evol. 28(2011) 2731-2739.
[18] W. Bejar, V. Gabriel, M. Amari, S. Morel, M. Mezghani, E. Maguin, C. Fontagné- Faucher, S. Bejar, H. Chouayekh, Characterization of glucansucrase and dextran from Weissella sp. TN610 with potential as safe food additives, Int. J. Biol. Macromol. 52(2013) 125-132.
[19] A. Romaní, R. Yáñez, G. Garrote, J.L. Alonso, J.C. Parajó, Sugar production from cellulosic biosludges generated in a water treatment plant of a Kraft pulp mill, Biochem. Eng. J. 37(3) (2007) 319-327.
[20] B.J. Tindall, R. Rosselló-Móra, H.J. Busse, W. Ludwig, P. Kämpfer, Notes on the characterization of prokaryote strains for taxonomic purposes, Int. J. Syst. Evol. Microbiol. 60(2010) 249-266.
[21] M. Santos, A. Rodrigues, J.A. Teixeira, Production of dextran and fructose from carob pod extract and cheese whey by Leuconostoc mesenteroides NRRL B512(f), Biochem. Eng. J. 25(1) (2005) 1-6.
[22] W. Zhang, J. Zhu, X. Zhu, M. Song, T. Zhang, F. Xin, W. Dong, J. Ma, M. Jiang, Expression of global regulator IrrE for improved succinate production under high salt stress by Escherichia coli, Bioresour. Technol. 254(2018) 151-156.
[23] W. Zhang, Y. Tao, M. Wu, F. Xin, W. Dong, J. Zhou, J. Gu, J. Ma, M. Jiang, Adaptive evolution improves acid tolerance and succinic acid production in Actinobacillus succinogenes, Process Biochem. 98(2020) 76-82.
[24] B.H.A. Rehm, Bacterial polymers:biosynthesis, modifications and applications, Nat. Rev. Microbiol. 8(2010) 578-592.
[25] R. Du, X. Qiao, F. Zhao, Q. Song, Q. Zhou, Y.u. Wang, L. Pan, Y.e. Han, Z. Zhou, Purification, characterization and antioxidant activity of dextran produced by Leuconostoc pseudomesenteroides from homemade wine, Carbohydr. Polym. 198(2018) 529-536.
[26] P.Y. Zhang, L.N. Zhang, S.Y. Cheng, Chemical structure and molecular weights of α-(1→3)-D-Glucan from Lentinus edodes, Biosci. Biotechnol. Biochem. 63(1999) 1197-1202.
[27] K.I. Shingel, Determination of structural peculiarities of dexran, pullulan and cirradiated pullulan by Fourier-transform IR spectroscopy, Carbohydr. Res. 337(16) (2002) 1445-1451.
[28] M.-S. Bounaix, V. Gabriel, S. Morel, H. Robert, P. Rabier, M. Remaud-Siméon, B. Gabriel, C. Fontagné-Faucher, Biodiversity of exopolysaccharides produced from sucrose by sourdough lactic acid bacteria, J. Agric. Food Chem. 57(22) (2009) 10889-10897.
[29] R.K. Purama, P. Goswami, A.T. Khan, A. Goyal, Structural analysis and properties of dextran produced by Leuconostoc mesenteroides NRRL B-640, Carbohydr. Polym. 76(1) (2009) 30-35.
[30] F.R. Seymour, R.D. Knapp, E.C.M. Chen, A. Jeanes, S.H. Bishop, Structural analysis of dextrans containing 2-O-α-d-glucosylated α-d-glucopyranosyl residues at the branch points, by use of ¹³C-nuclear magnetic resonance spectroscopy and gas-liquid chromatography-mass spectrometry, Carbohydr. Res. 71(1) (1979) 231-250.
[31] F.R. Seymour, R.D. Knapp, S.H. Bishop, Correlation of the structure of dextrans to their ¹H-n.m.r. Spectra, Carbohydr. Res. 74(1) (1979) 77-92.
[32] N.H. Maina, M. Tenkanen, H. Maaheimo, R. Juvonen, L. Virkki, NMR spectroscopic analysis of exopolysaccharides produced by Leuconostoc citreum and Weissella confusa, Carbohydr. Res. 343(9) (2008) 1446-1455.
[33] K. Wangpaiboon, P. Padungros, S. Nakapong, T. Charoenwongpaiboon, M. Rejzek, R.A. Field, R. Pichyangkura, An α-1,6-and α-1,3-linked glucan produced by Leuconostoc citreum ABK-1 alternansucrase with nanoparticle and filmforming properties, Sci. Rep. 8(2018) 8340.
[34] R. Shukla, S. Shukla, V. Bivolarski, I. Iliev, I. Ivanova, A. Goyal, Structural characterization of insoluble dextran produced by Leuconostoc mesenteroides NRRL B-1149 in the presence of maltose, Food Technol. Biotechnol. 3(2011) 291-296.
[35] P.A. Padmanabhan, D.-S. Kim, Production of insoluble dextran using cell-bound dextransucrase of Leuconostoc mesenteroides NRRL B-523, Carbohydr. Res. 337(17) (2002) 1529-1533.
[36] G.L. Côté, T.D. Leathers, Insoluble glucans from planktonic and biofilm cultures of mutants of Leuconostoc mesenteroides NRRL B-1355, Appl. Microbiol. Biotechnol. 82(1) (2009) 149-154.
[37] W.A. Belli, R.E. Marquis, Catabolite modification of acid tolerance of Streptococcus mutans GS-5, Oral Microbiol. Immunol. 9(1) (1994) 29-34.
[38] Z. Ren, L.L. Chen, J.Y. Li, Y.Q. Li, Inhibition of Streptococcus mutans polysaccharide synthesis by molecules targeting glycosyltransferase activity, J. Oral Microbiol. 8(2016) 31095.
[39] R. Du, X. Qiao, Y.u. Wang, B.o. Zhao, Y.e. Han, Z. Zhou, Determination of glucansucrase encoding gene in Leuconostoc mesenteroides, Int. J. Biol. Macromol. 137(2019) 761-766.

Production and characterization of insoluble α-1,3-linked glucan and soluble α-1,6-linked dextran from Leuconostoc pseudomesenteroides G29

Production and characterization of insoluble α-1,3-linked glucan and soluble α-1,6-linked dextran from Leuconostoc pseudomesenteroides G29

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics

本文评价