SCI和EI收录∣中国化工学会会刊

中国化学工程学报 ›› 2022, Vol. 49 ›› Issue (9): 130-139.DOI: 10.1016/j.cjche.2022.05.011

• Special Column: Membranes for Life Science • 上一篇    下一篇

Purifying chylous plasma by precluding triglyceride via carboxylated polyethersulfone microfiltration membrane

Yang Liu1,2, Qiu Han1, Guiliang Li1,2, Haibo Lin1, Fu Liu1,2, Gang Deng3, Dingfeng Lv4, Weijie Sun4   

  1. 1. Ningbo Institute of Materials Technology & Engineering, Chinese Academy of Sciences, Ningbo 315000, China;
    2. University of Chinese Academy of Sciences, Beijing 100049, China;
    3. The Ningbo Central Blood Station, Ningbo 315099, China;
    4. Department of Blood Transfusion, Ningbo First Hospital, Ningbo 315010, China
  • 收稿日期:2021-09-29 修回日期:2022-04-30 发布日期:2022-10-19
  • 通讯作者: Fu Liu,E-mail:fu.liu@nimte.ac.cn
  • 基金资助:
    This work is financially supported by Natural Science Foundation of Ningbo City (2018A610026), Zhejiang Provincial Natural Science Foundation of China for Distinguished Young Scholars (LR20E030002), Ten thousand plan-high level talents special support plan of Zhejiang province, China (ZJWR0108020) and Youth Innovation Promotion Association of Chinese Academy of Science (2014258).

Purifying chylous plasma by precluding triglyceride via carboxylated polyethersulfone microfiltration membrane

Yang Liu1,2, Qiu Han1, Guiliang Li1,2, Haibo Lin1, Fu Liu1,2, Gang Deng3, Dingfeng Lv4, Weijie Sun4   

  1. 1. Ningbo Institute of Materials Technology & Engineering, Chinese Academy of Sciences, Ningbo 315000, China;
    2. University of Chinese Academy of Sciences, Beijing 100049, China;
    3. The Ningbo Central Blood Station, Ningbo 315099, China;
    4. Department of Blood Transfusion, Ningbo First Hospital, Ningbo 315010, China
  • Received:2021-09-29 Revised:2022-04-30 Published:2022-10-19
  • Contact: Fu Liu,E-mail:fu.liu@nimte.ac.cn
  • Supported by:
    This work is financially supported by Natural Science Foundation of Ningbo City (2018A610026), Zhejiang Provincial Natural Science Foundation of China for Distinguished Young Scholars (LR20E030002), Ten thousand plan-high level talents special support plan of Zhejiang province, China (ZJWR0108020) and Youth Innovation Promotion Association of Chinese Academy of Science (2014258).

摘要: Precluding the excessive lipoproteins from plasma rapidly and effectively is highly needed for biomedical detection and reducing plasma product scrap in blood donation stations. The current centrifugation procedure is high-cost and time-consuming. Herein, we fabricated an anionic microfiltration polyethersulfone (PES) membrane modified by interface swelling and implanting of acrylic acid (AA) for screening out large particle lipoprotein chylomicron (CM) and adsorbing cationic very low-density lipoproteins (VLDL). To improve the separation efficiency, a two-stage filtration through carboxylated polyethersulfone microfiltration membranes with the mean pore size of 0.45 and 0.22 μm respectively were conducted. Attenuated total reflection Fourier transform infrared technique (ATR-FTIR), water contact angle (WCA), Zeta potential and scanning electron microscope (SEM) were employed to characterize the modified membrane. To test the effectiveness of this membrane, plasma flux and concentration variation of plasma components were examined to study the purification effectiveness. Furthermore, the hemocompatibility of modified membranes was tested to confirm its practicability on blood-contacting materials. The carboxylated polyethersulfone microfiltration membrane shows its promising potential application to purify chylous plasma.

关键词: Chylous plasma, Chylomicrons, Very low-density lipoproteins, Carboxylated polyethersulfone microfiltration membrane

Abstract: Precluding the excessive lipoproteins from plasma rapidly and effectively is highly needed for biomedical detection and reducing plasma product scrap in blood donation stations. The current centrifugation procedure is high-cost and time-consuming. Herein, we fabricated an anionic microfiltration polyethersulfone (PES) membrane modified by interface swelling and implanting of acrylic acid (AA) for screening out large particle lipoprotein chylomicron (CM) and adsorbing cationic very low-density lipoproteins (VLDL). To improve the separation efficiency, a two-stage filtration through carboxylated polyethersulfone microfiltration membranes with the mean pore size of 0.45 and 0.22 μm respectively were conducted. Attenuated total reflection Fourier transform infrared technique (ATR-FTIR), water contact angle (WCA), Zeta potential and scanning electron microscope (SEM) were employed to characterize the modified membrane. To test the effectiveness of this membrane, plasma flux and concentration variation of plasma components were examined to study the purification effectiveness. Furthermore, the hemocompatibility of modified membranes was tested to confirm its practicability on blood-contacting materials. The carboxylated polyethersulfone microfiltration membrane shows its promising potential application to purify chylous plasma.

Key words: Chylous plasma, Chylomicrons, Very low-density lipoproteins, Carboxylated polyethersulfone microfiltration membrane