SCI和EI收录∣中国化工学会会刊

中国化学工程学报 ›› 2023, Vol. 59 ›› Issue (7): 128-134.DOI: 10.1016/j.cjche.2022.11.015

• Full Length Article • 上一篇    下一篇

Towards understanding the mechanism of n-hexane tolerance in Synechocystis sp. PCC 6803

Tao Sun1,2,3, Shubin Li1,2, Guangsheng Pei1,2, Lei Chen1,2, Weiwen Zhang1,2,3   

  1. 1. Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, China;
    2. Frontier Science Center for Synthetic Biology & Key Laboratory of Systems Bioengineering, Ministry of Education of China, Tianjin 300072, China;
    3. Center for Biosafety Research and Strategy, Tianjin University, Tianjin 300072, China
  • 收稿日期:2022-10-22 修回日期:2022-11-18 出版日期:2023-07-28 发布日期:2023-10-14
  • 通讯作者: Lei Chen,E-mail:lchen@tju.edu.cn;Weiwen Zhang,E-mail:wwzhang8@tju.edu.cn
  • 基金资助:
    This research was supported by grants from the National Key Research and Development Program of China (2020YFA0906800, 2021YFA0909700, 2018YFA0903600 and 2019YFA0904600).

Towards understanding the mechanism of n-hexane tolerance in Synechocystis sp. PCC 6803

Tao Sun1,2,3, Shubin Li1,2, Guangsheng Pei1,2, Lei Chen1,2, Weiwen Zhang1,2,3   

  1. 1. Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, China;
    2. Frontier Science Center for Synthetic Biology & Key Laboratory of Systems Bioengineering, Ministry of Education of China, Tianjin 300072, China;
    3. Center for Biosafety Research and Strategy, Tianjin University, Tianjin 300072, China
  • Received:2022-10-22 Revised:2022-11-18 Online:2023-07-28 Published:2023-10-14
  • Contact: Lei Chen,E-mail:lchen@tju.edu.cn;Weiwen Zhang,E-mail:wwzhang8@tju.edu.cn
  • Supported by:
    This research was supported by grants from the National Key Research and Development Program of China (2020YFA0906800, 2021YFA0909700, 2018YFA0903600 and 2019YFA0904600).

摘要: Synthetic biology efforts have also led to the development of photosynthetic cyanobacteria as “autotrophic cell factories” for biosynthesis of various biofuels directly from CO2. However, the low tolerance to toxicity of biofuels has restricted the economic application of cyanobacterial hosts. In this study, RNA-seq transcriptomics was employed to reveal stress responses to exogenous n-hexane in Synechocystis sp. PCC 6803. Functional enrichment analysis of the transcriptomic data showed that signal transduction systems were induced significantly. To further identify regulatory genes related to n-hexane tolerance, a library of transcriptional regulators (TRs) deletion mutants was then screened for their roles in n-hexane tolerance. The results showed that a knockout mutant of slr0724 that encodes an HtaR suppressor protein was more tolerant to n-hexane than the wild type, indicating the involvement of slr0724 in n-hexane tolerance. This study provides the foundation for better understanding the cellular responses to n-hexane in Synechocystis sp. PCC 6803, which could contribute to the further engineering of n-hexane tolerance in cyanobacteria.

关键词: Transcriptome, n-Hexane tolerance, Metabolomics, Synechocystis

Abstract: Synthetic biology efforts have also led to the development of photosynthetic cyanobacteria as “autotrophic cell factories” for biosynthesis of various biofuels directly from CO2. However, the low tolerance to toxicity of biofuels has restricted the economic application of cyanobacterial hosts. In this study, RNA-seq transcriptomics was employed to reveal stress responses to exogenous n-hexane in Synechocystis sp. PCC 6803. Functional enrichment analysis of the transcriptomic data showed that signal transduction systems were induced significantly. To further identify regulatory genes related to n-hexane tolerance, a library of transcriptional regulators (TRs) deletion mutants was then screened for their roles in n-hexane tolerance. The results showed that a knockout mutant of slr0724 that encodes an HtaR suppressor protein was more tolerant to n-hexane than the wild type, indicating the involvement of slr0724 in n-hexane tolerance. This study provides the foundation for better understanding the cellular responses to n-hexane in Synechocystis sp. PCC 6803, which could contribute to the further engineering of n-hexane tolerance in cyanobacteria.

Key words: Transcriptome, n-Hexane tolerance, Metabolomics, Synechocystis