Investigates of substrate mingling ratio and organic loading rate of KOH pretreated corn stover and pig manure in batch and semi-continuous system: Anaerobic digestion performance and microbial characteristics

doi:10.1016/j.cjche.2023.04.014

中国化学工程学报 ›› 2023, Vol. 62 ›› Issue (10): 114-123.DOI: 10.1016/j.cjche.2023.04.014

• Full Length Article • 上一篇下一篇

Investigates of substrate mingling ratio and organic loading rate of KOH pretreated corn stover and pig manure in batch and semi-continuous system: Anaerobic digestion performance and microbial characteristics

Chenyang Zhu¹, Ruoran Qu², Xiujin Li¹, Xiaoyu Zuo¹, Hairong Yuan¹

1. State Key Laboratory of Chemical Resource Engineering, Department of Environmental Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, China;
2. Sinochem Commerce Co., Ltd, Beijing 100045, China

收稿日期:2022-12-27 修回日期:2023-04-09 出版日期:2023-10-28 发布日期:2023-12-23
通讯作者: Xiujin Li,E-mail:xjli@mail.buct.edu.cn;Hairong Yuan,E-mail:yuanhairong75@163.com
基金资助:
The authors are grateful to the fund supports from the Fundamental Research Funds for the Central Universities (JD2326).

Investigates of substrate mingling ratio and organic loading rate of KOH pretreated corn stover and pig manure in batch and semi-continuous system: Anaerobic digestion performance and microbial characteristics

Chenyang Zhu¹, Ruoran Qu², Xiujin Li¹, Xiaoyu Zuo¹, Hairong Yuan¹

1. State Key Laboratory of Chemical Resource Engineering, Department of Environmental Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, China;
2. Sinochem Commerce Co., Ltd, Beijing 100045, China

Received:2022-12-27 Revised:2023-04-09 Online:2023-10-28 Published:2023-12-23
Contact: Xiujin Li,E-mail:xjli@mail.buct.edu.cn;Hairong Yuan,E-mail:yuanhairong75@163.com
Supported by:
The authors are grateful to the fund supports from the Fundamental Research Funds for the Central Universities (JD2326).

摘要/Abstract

摘要： The effects of substrate mingling ratio (SMR) (1:1, 1:2, 1:3, 3:1, and 2:1) and organic loading rate (OLR) (50–90 g total solids per liter per day) on anaerobic co-digestion performance and microbial characteristics were investigated for pig manure (PM) and pretreated/untreated corn stover in batch and semi-continuous anaerobic digestion (AD) system. The results showed that SMR and pretreatment affected co-digestion performance. The maximum cumulative methane yield of 428.5 ml·g^-1 (based on volatile solids (VS)) was obtained for PC_P13, which was 35.7% and 40.0% higher than that of CS_U and PM. In the first 5 days, the maximum methane yield improvement rate was 378.1% for PC_P13. The daily methane yield per gram VS of PC_P13 was 11.4%–18.5% higher than that of PC_U13. Clostridium_sensu_stricto_1, DMER64, and Bacteroides and Methanosaeta, Methanobacterium, and Methanospirillum had higher relative abundance at the genus level. Therefore, SMR and OLR are important factor affecting the AD process, and OLR can affect methane production through volatile fatty acids.

关键词: Substrate mingling ratio, Organic loading rate, Co-digestion, Corn stover, Pig manure, Microbial community

Abstract: The effects of substrate mingling ratio (SMR) (1:1, 1:2, 1:3, 3:1, and 2:1) and organic loading rate (OLR) (50–90 g total solids per liter per day) on anaerobic co-digestion performance and microbial characteristics were investigated for pig manure (PM) and pretreated/untreated corn stover in batch and semi-continuous anaerobic digestion (AD) system. The results showed that SMR and pretreatment affected co-digestion performance. The maximum cumulative methane yield of 428.5 ml·g^-1 (based on volatile solids (VS)) was obtained for PC_P13, which was 35.7% and 40.0% higher than that of CS_U and PM. In the first 5 days, the maximum methane yield improvement rate was 378.1% for PC_P13. The daily methane yield per gram VS of PC_P13 was 11.4%–18.5% higher than that of PC_U13. Clostridium_sensu_stricto_1, DMER64, and Bacteroides and Methanosaeta, Methanobacterium, and Methanospirillum had higher relative abundance at the genus level. Therefore, SMR and OLR are important factor affecting the AD process, and OLR can affect methane production through volatile fatty acids.

Key words: Substrate mingling ratio, Organic loading rate, Co-digestion, Corn stover, Pig manure, Microbial community

Chenyang Zhu, Ruoran Qu, Xiujin Li, Xiaoyu Zuo, Hairong Yuan. Investigates of substrate mingling ratio and organic loading rate of KOH pretreated corn stover and pig manure in batch and semi-continuous system: Anaerobic digestion performance and microbial characteristics[J]. 中国化学工程学报, 2023, 62(10): 114-123.

参考文献

[1] Y.F. Wei, Y.Y. Lan, X.J. Li, M.H. Gao, S. Yuan, H.R. Yuan, Effect of wheat straw pretreated with liquid fraction of digestate from different substrates on anaerobic digestion performance and microbial community characteristics, Sci. Total Environ. 818 (2022) 151764.
[2] M.J. Taherzadeh, K. Karimi, Pretreatment of lignocellulosic wastes to improve ethanol and biogas production: A review, Int. J. Mol. Sci. 9 (9) (2008) 1621–1651.
[3] J. Li, A.C. Wachemo, H.R. Yuan, X.Y. Zuo, X.J. Li, Natural freezing-thawing pretreatment of corn stalk for enhancing anaerobic digestion performance, Bioresour. Technol. 288 (2019) 121518.
[4] Z.H. Yong, Y.L. Dong, X. Zhang, T.W. Tan, Anaerobic co-digestion of food waste and straw for biogas production, Renew. Energy 78 (2015) 527–530.
[5] D.Y. Zhao, B.H. Yan, C. Liu, B. Yao, L. Luo, Y. Yang, L.C. Liu, F. Wu, Y.Y. Zhou, Mitigation of acidogenic product inhibition and elevated mass transfer by biochar during anaerobic digestion of food waste, Bioresour. Technol. 338 (2021) 125531.
[6] X. Wu, W.Y. Yao, J. Zhu, C. Miller, Biogas and CH₄ productivity by co-digesting swine manure with three crop residues as an external carbon source, Bioresour. Technol. 101 (11) (2010) 4042–4047.
[7] B.Y. Xiao, X.Y. Tang, W.Z. Zhang, K. Zhang, T. Yang, Y.P. Han, J.X.Liu, Effects of rice straw ratio on mesophilic and thermophilic anaerobic co-digestion of swine manure and rice straw mixture, Energy 239 (2022) 122021.
[8] S.Y. Pan, H.M. Zabed, Z.C. Li, X.H. Qi, Y.T. Wei, Enrichment and balancing of nutrients for improved methane production using three compositionally different agro-livestock wastes: Process performance and microbial community analysis, Bioresour. Technol. 357 (2022) 127360.
[9] J.Q. Ye, D. Li, Y.M. Sun, G.H. Wang, Z.H. Yuan, F. Zhen, Y.Wang, Improved biogas production from rice straw by co-digestion with kitchen waste and pig manure, Waste Manag. 33 (12) (2013) 2653–2658.
[10] S. Buragohain, P. Mahanta, K. Mohanty, Biogas production from anaerobic mono- and co-digestion of lignocellulosic feedstock: Process optimization and its implementation at community level, Environ. Technol. Innov. 24 (2021) 101981.
[11] D. Li, S.C. Liu, L. Mi, Z.D. Li, Y.X. Yuan, ZY. Yan, X.F. Liu, Effects of feedstock ratio and organic loading rate on the anaerobic mesophilic co-digestion of rice straw and pig manure, Bioresour. Technol. 187 (2015) 120–127.
[12] R.L. Guan, H.R. Yuan, S. Yuan, B.B. Yan, X.Y. Zuo, X.T. Chen, X.J.Li, Current development and perspectives of anaerobic bioconversion of crop stalks to Biogas: A review, Bioresour. Technol. 349 (2022) 126615.
[13] R. Guan, H. Yuan, L. Zhang, X. Zuo, X. Li, Combined pretreatment using CaO and liquid fraction of digestate of rice straw: Anaerobic digestion performance and electron transfer, Chin. J. Chem. Eng. 36 (2021) 223–232.
[14] M. Stiven Romero-Güiza, R. Wahid, V. Hernández, H. Møller, B.Fernández, Improvement of wheat straw anaerobic digestion through alkali pre-treatment: Carbohydrates bioavailability evaluation and economic feasibility, Sci. Total Environ. 595 (2017) 651–659.
[15] A. Mahdy, S. Bi, Y. Song, W. Qiao, R. Dong, Overcome inhibition of anaerobic digestion of chicken manure under ammonia-stressed condition by lowering the organic loading rate, Bioresour. Technol. Rep. 9 (2020) 100359.
[16] Y. Wei, Y. Gao, H. Yuan, Y. Chang, X. Li, Effects of organic loading rate and pretreatments on digestion performance of corn stover and chicken manure in completely stirred tank reactor (CSTR), Sci. Total Environ. 815 (2022) 152499.
[17] M. Hassan, C. Zhao, W. Ding, Enhanced methane generation and biodegradation efficiencies of goose manure by thermal-sonication pretreatment and organic loading management in CSTR, Energy 198 (2020) 117370.
[18] J. Ning, M.D. Zhou, X.F. Pan, C.X. Li, N. Lv, T. Wang, G.J. Cai, R.M. Wang, J.J. Li, G.F.Zhu, Simultaneous biogas and biogas slurry production from co-digestion of pig manure and corn straw: Performance optimization and microbial community shift, Bioresour. Technol. 282 (2019) 37–47.
[19] X.M. Wang, Z.F. Li, X. Bai, X.Q. Zhou, S.K. Cheng, R.L. Gao, J.C.Sun, Study on improving anaerobic co-digestion of cow manure and corn straw by fruit and vegetable waste: Methane production and microbial community in CSTR process, Bioresour. Technol. 249 (2018) 290–297.
[20] Y. Hu, X. Hao, J. Wang, Y. Cao, Enhancing anaerobic digestion of lignocellulosic materials in excess sludge by bioaugmentation and pre-treatment, Waste Manag. 49 (2016) 55–63.
[21] M. Jaffar, Y.Z. Pang, H.R. Yuan, D.X. Zou, Y.P. Liu, B.N. Zhu, R.M. Korai, X.J.Li, Wheat straw pretreatment with KOH for enhancing biomethane production and fertilizer value in anaerobic digestion, Chin. J. Chem. Eng. 24 (3) (2016) 404–409.
[22] Y. Wei, X. Li, L. Yu, D. Zou, H. Yuan, Mesophilic anaerobic co-digestion of cattle manure and corn stover with biological and chemical pretreatment, Bioresour. Technol. 198 (2015) 431–436.
[23] F.W. Gilcreas, Standard methods for the examination of water and waste water, Am. J. Public Health Nation’s Health 56 (3) (1966) 387–388.
[24] T. Liu, X. Zhou, Z. Li, X. Wang, J. Sun, Effects of liquid digestate pretreatment on biogas production for anaerobic digestion of wheat straw, Bioresour. Technol. 280 (2019) 345–351.
[25] R.A. Labatut, L.T. Angenent, N.R.Scott, Biochemical methane potential and biodegradability of complex organic substrates, Bioresour. Technol. 102 (3) (2011) 2255–2264.
[26] W.J. Park, J.H. Ahn, S. Hwang, C.K. Lee, Effect of output power, target temperature, and solid concentration on the solubilization of waste activated sludge using microwave irradiation, Bioresour. Technol. 101 (1) (2010) S13–S16.
[27] M. Wang, Y. Liu, X.R. Jiang, J.N. Fang, Q.Y. Lyu, X.H. Wang, Z.Y.Yan, Multi-omics reveal the structure and function of microbial community in co-digestion of corn straw and pig manure, J. Clean. Prod. 322 (2021) 129150.
[28] E.S. Gaballah, A. El-Fatah Abomohra, C. Xu, M. Elsayed, T.K. Abdelkader, J.C. Lin, Q.X.Yuan, Enhancement of biogas production from rape straw using different co-pretreatment techniques and anaerobic co-digestion with cattle manure, Bioresour. Technol. 309 (2020) 123311.
[29] R.L. Guan, J.Y. Gu, A.C. Wachemo, H.R. Yuan, X.J.Li, Novel insights into anaerobic digestion of rice straw using combined pretreatment with CaO and the liquid fraction of digestate: Anaerobic digestion performance and kinetic analysis, Energy Fuels 34 (2) (2020) 1119–1130.
[30] Y.F. Wei, H.R. Yuan, A.C. Wachemo, X.J.Li, Impacts of modification of corn stover on the synergistic effect and microbial community structure of Co-digestion with chicken manure, Energy Fuels 34 (1) (2020) 401–411.
[31] R. Karki, W. Chuenchart, K.C. Surendra, S. Shrestha, L. Raskin, S. Sung, A. Hashimoto, S.Kumar Khanal, Anaerobic co-digestion: Current status and perspectives, Bioresour. Technol. 330 (2021) 125001.
[32] H.R. Yuan, R.P. Li, Y.T. Zhang, X.J. Li, C.M. Liu, Y. Meng, M.N. Lin, Z.Y. Yang, Anaerobic digestion of ammonia-pretreated corn stover, Biosyst. Eng. 129 (2015) 142–148.
[33] A. Hejnfelt, I.Angelidaki, Anaerobic digestion of slaughterhouse by-products, Biomass Bioenergy 33 (8) (2009) 1046–1054.
[34] N.N. Zhai, T. Zhang, D.X. Yin, G.H. Yang, X.J. Wang, G.X. Ren, Y.Z.Feng, Effect of initial pH on anaerobic co-digestion of kitchen waste and cow manure, Waste Manag. 38 (2015) 126–131.
[35] R.L. Zhu, L.Y. He, Q.Y. Li, T.X. Huang, M. Gao, Q. Jiang, J.Y. Liu, A.R. Cai, D.Z. Shi, L. Gu, Q.He, Mechanism study of improving anaerobic co-digestion performance of waste activated sludge and food waste by Fe₃O₄, J. Environ. Manag. 300 (2021) 113745.
[36] L. Wang, Q. Zhou, G.H.Zheng, Comprehensive analysis of the factors for propionic acid accumulation in acidogenic phase of anaerobic process, Environ. Technol. 27 (3) (2006) 269–276.
[37] R. Bao, Y. Wei, R. Guan, X. Li, X. Lu, S. Rong, X. Zuo, H. Yuan, High-solids anaerobic co-digestion performances and microbial community dynamics in co-digestion of different mixing ratios with food waste and highland barley straw, Energy 262 (2023) 125529.
[38] Z. Shi, L. Zhang, H. Yuan, X. Li, Y. Chang, X. Zuo, Oyster shells improve anaerobic dark fermentation performances of food waste: Hydrogen production, acidification performances, and microbial community characteristics, Bioresour. Technol. 335 (2021) 125268.
[39] J.W. Lim, J.A. Chiam, J.Y. Wang, Microbial community structure reveals how microaeration improves fermentation during anaerobic co-digestion of brown water and food waste, Bioresour. Technol. 171 (2014) 132–138.
[40] H. Mu, C. Zhao, Y. Zhao, Y. Li, D. Hua, X. Zhang, H. Xu, Enhanced methane production by semi-continuous mesophilic co-digestion of potato waste and cabbage waste: Performance and microbial characteristics analysis, Bioresour. Technol. 236 (2017) 68–76.
[41] S.Q. Wang, G.M. Zhang, P.Y. Zhang, X.W. Ma, F. Li, H.B. Zhang, X. Tao, J.P. Ye, M.Nabi, Rumen fluid fermentation for enhancement of hydrolysis and acidification of grass clipping, J. Environ. Manag. 220 (2018) 142–148.
[42] M. Navarro-Díaz, I. Valdez-Vazquez, A.E.Escalante, Ecological perspectives of hydrogen fermentation by microbial consortia: What we have learned and the way forward, Int. J. Hydrog. Energy 41 (39) (2016) 17297–17308.
[43] Y. Deng, X. Guo, Y.W. Wang, M.X. He, K.D. Ma, H.M. Wang, X.R. Chen, D.L. Kong, Z.R. Yang, Z.Y.Ruan, Terrisporobacter petrolearius sp. nov., isolated from an oilfield petroleum reservoir, Int. J. Syst. Evol. Microbiol. 65 (Pt_10) (2015) 3522–3526.
[44] A. Fey, R. Conrad, Effect of temperature on carbon and electron flow and on the archaeal community in methanogenic rice field soil, Appl. Environ. Microbiol. 66 (11) (2000) 4790–4797.
[45] Y. Wei, H. Yuan, A.C. Wachemo, X. Li, Anaerobic co-digestion of cattle manure and liquid fraction of digestate (LFD) pretreated corn stover: Pretreatment process optimization and evolution of microbial community structure, Bioresour. Technol. 296 (2020) 122282.
[46] L.W. Wu, Y.F. Yang, S. Chen, M.X. Zhao, Z.W. Zhu, S.H. Yang, Y.Y. Qu, Q. Ma, Z.L. He, J.Z. Zhou, Q.He, Long-term successional dynamics of microbial association networks in anaerobic digestion processes, Water Res. 104 (2016) 1–10.

Investigates of substrate mingling ratio and organic loading rate of KOH pretreated corn stover and pig manure in batch and semi-continuous system: Anaerobic digestion performance and microbial characteristics

Investigates of substrate mingling ratio and organic loading rate of KOH pretreated corn stover and pig manure in batch and semi-continuous system: Anaerobic digestion performance and microbial characteristics

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 11

编辑推荐

Metrics

本文评价

[1]	Hu Chen, Ying Wang, Puyu Wang, Yongkang Lv. Assessing quinoline removal performances of an aerobic continuous moving bed biofilm reactor (MBBR) bioaugmented with Pseudomonas citronellolis LV1[J]. 中国化学工程学报, 2023, 57(5): 132-140.
[2]	Mengmeng Wang, Gang Cao, Naixian Feng, Yongzhang Pan. Bioaugmentation of two-stage aerobic sequencing batch reactor with mixed strains for high nitrate nitrogen wastewater treatment[J]. 中国化学工程学报, 2020, 28(12): 3103-3109.
[3]	Xiaoyu Zuo, Hairong Yuan, Akiber Chufo Wachemo, Xitong Wang, Liang Zhang, Juan Li, Hongliang Wen, Jiaxi Wang, Xiujin Li. The relationships among sCOD, VFAs, microbial community, and biogas production during anaerobic digestion of rice straw pretreated with ammonia[J]. 中国化学工程学报, 2020, 28(1): 286-292.
[4]	Hairong Yuan, Yanyan Lan, Jialin Zhu, Akiber Chufo Wachemo, Xiujin Li, Liang Yu. Effect on anaerobic digestion performance of corn stover by freezing-thawing with ammonia pretreatment[J]. Chinese Journal of Chemical Engineering, 2019, 27(1): 200-207.
[5]	Meinan Zhen, Benru Song, Xiaomei Liu, Radhika Chandankere, Jingchun Tang. Biochar-mediated regulation of greenhouse gas emission and toxicity reduction in bioremediation of organophosphorus pesticide-contaminated soils[J]. Chinese Journal of Chemical Engineering, 2018, 26(12): 2592-2600.
[6]	Huisheng Lü, Xingfang Shi, Yonghui Li, Fanmei Meng, Shuangyan Liu, Li Yan. Multi-objective regulation in autohydrolysis process of corn stover by liquid hot water pretreatment[J]. , 2017, 25(4): 499-506.
[7]	Zhiqiang Zhang, Shanying Hu, Dingjiang Chen, Bing Zhu. An analysis of an ethanol-based, whole-crop refinery system in China[J]. Chinese Journal of Chemical Engineering, 2016, 24(11): 1609-1618.
[8]	吕惠生, 任苗苗, 张敏华, 陈莹. Pretreatment of Corn Stover Using Supercritical CO₂ with Water-Ethanol as Co-solvent[J]. Chinese Journal of Chemical Engineering, 2013, 21(5): 551-557.
[9]	雷鸣柳, 张红漫, 郑洪波, 李媛媛, 黄和, 徐蓉. Characterization of Lignins Isolated from Alkali Treated Prehydrolysate of Corn Stover[J]. Chinese Journal of Chemical Engineering, 2013, 21(4): 427-433.
[10]	袁海荣, 庞云芝, 王奎升, 刘研萍, 左晓宇, 马淑勍, 李秀金. 秸秆固化成型燃料引火助燃剂的点火和烟气排放特性[J]. , 2010, 18(4): 687-694.
[11]	高鹏飞, 范代娣, 骆艳娥, 马晓轩, 马沛, 惠俊峰, 朱晨辉. Efficient and Comprehensive Utilization of Hemicellulose in the Corn Stover[J]. , 2009, 17(2): 350-354.