Influence of Conditions on Reuterin Accumulation by the Resting Cell Biotransformation Process

›› 2011, Vol. 19 ›› Issue (6): 1023-1027.

Influence of Conditions on Reuterin Accumulation by the Resting Cell Biotransformation Process

陈国, 杨道茂, 肖雅琴, 陈宏文

Department of Bioengineering and Biotechnology, Huaqiao University, Xiamen 361021, China

收稿日期:2010-09-20 修回日期:2011-08-18 出版日期:2011-12-28 发布日期:2012-04-24
通讯作者: CHEN Guo, E-mail: chenguo@hqu.edu.cn
基金资助:
Supported by the National Natural Science Foundation of China (20906035);the Fundamental Research Funds for the Central Universities (JB-JX1002)

Influence of Conditions on Reuterin Accumulation by the Resting Cell Biotransformation Process

CHEN Guo, YANG Daomao, XIAO Yaqin, CHEN Hongwen

Department of Bioengineering and Biotechnology, Huaqiao University, Xiamen 361021, China

Received:2010-09-20 Revised:2011-08-18 Online:2011-12-28 Published:2012-04-24
Supported by:
Supported by the National Natural Science Foundation of China (20906035);the Fundamental Research Funds for the Central Universities (JB-JX1002)

摘要/Abstract

摘要： 3-Hydroxypropionaldehyde (3-HPA) is a potential valuable chemical and new broad-spectrum antimicrobial substance. In order to improve the conversion of 3-HPA/glycerol, our work studied the two-step process from glycerol to 3-HPA, and investigated the influence of cell harvest time, glycerol concentration, biomass con-centration, pH and temperature on the production of 3-HPA by Lactobacillus reuteri CG001, respectively. The results showed that molar conversion yield of 3-HPA/glycerol reached 97.9% under the condition that 200 mmol·L^-1 glycerol was converted by 25.3 g·L^-1 resting cell for 1 h at 30℃. The cells could not be reused directly because the L. reuteri almost lost its bioconversion activity completely, but the ability of glycerol conversion could gradually recover if the fresh medium was added to the deactivated cell for 4 h.

关键词: Lactobacillus reuteri, 3-hydroxypropionaldehyde, reuterin, bioconversion

Abstract: 3-Hydroxypropionaldehyde (3-HPA) is a potential valuable chemical and new broad-spectrum antimicrobial substance. In order to improve the conversion of 3-HPA/glycerol, our work studied the two-step process from glycerol to 3-HPA, and investigated the influence of cell harvest time, glycerol concentration, biomass con-centration, pH and temperature on the production of 3-HPA by Lactobacillus reuteri CG001, respectively. The results showed that molar conversion yield of 3-HPA/glycerol reached 97.9% under the condition that 200 mmol·L^-1 glycerol was converted by 25.3 g·L^-1 resting cell for 1 h at 30℃. The cells could not be reused directly because the L. reuteri almost lost its bioconversion activity completely, but the ability of glycerol conversion could gradually recover if the fresh medium was added to the deactivated cell for 4 h.

Key words: Lactobacillus reuteri, 3-hydroxypropionaldehyde, reuterin, bioconversion

陈国, 杨道茂, 肖雅琴, 陈宏文. Influence of Conditions on Reuterin Accumulation by the Resting Cell Biotransformation Process[J]. , 2011, 19(6): 1023-1027.

CHEN Guo, YANG Daomao, XIAO Yaqin, CHEN Hongwen. Influence of Conditions on Reuterin Accumulation by the Resting Cell Biotransformation Process[J]. , 2011, 19(6): 1023-1027.

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Influence of Conditions on Reuterin Accumulation by the Resting Cell Biotransformation Process

Influence of Conditions on Reuterin Accumulation by the Resting Cell Biotransformation Process

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