Microfluidic reactor with immobilized enzyme-from construction to applications: A review

doi:10.1016/j.cjche.2020.12.011

摘要/Abstract

摘要： Microfluidic, as the systems for using microchannel (micron-or sub-micron scale) to process or manipulate microflow, is being widely applied in enzyme biotechnology and biocatalysis. Microfluidic immobilized enzyme reactor (MIER) is a tool with great value for the study of catalytic property and optimal reaction parameter in a flourishing and highly producing manner. In view of its advantages in efficiency, economy, and addressable recognition especially, MIER occupies an important position in the investigation of life science, including molecular biology, bioanalysis and biosensing, biocatalysis etc. Immobilization of enzymes can generally improve their stability, and upon most occasions, the immobilized enzyme is endowed with recyclability. In this review, the enzyme immobilization techniques applied in MIER will be discussed, followed by summarizing the novel developments in the field of MIER for biocatalysis, bioconversion and bioanalysis. The preponderances and deficiencies of the current state-of-the-art preparation ways of MIER are peculiarly discussed. In addition, the prospects of its future study are outlined.

关键词: Microfluidic immobilized enzyme reactor, Immobilization strategies, Biocatalysis, Bioconversion, Bioanalysis

Abstract: Microfluidic, as the systems for using microchannel (micron-or sub-micron scale) to process or manipulate microflow, is being widely applied in enzyme biotechnology and biocatalysis. Microfluidic immobilized enzyme reactor (MIER) is a tool with great value for the study of catalytic property and optimal reaction parameter in a flourishing and highly producing manner. In view of its advantages in efficiency, economy, and addressable recognition especially, MIER occupies an important position in the investigation of life science, including molecular biology, bioanalysis and biosensing, biocatalysis etc. Immobilization of enzymes can generally improve their stability, and upon most occasions, the immobilized enzyme is endowed with recyclability. In this review, the enzyme immobilization techniques applied in MIER will be discussed, followed by summarizing the novel developments in the field of MIER for biocatalysis, bioconversion and bioanalysis. The preponderances and deficiencies of the current state-of-the-art preparation ways of MIER are peculiarly discussed. In addition, the prospects of its future study are outlined.

Key words: Microfluidic immobilized enzyme reactor, Immobilization strategies, Biocatalysis, Bioconversion, Bioanalysis

Han Zhang, Yunpeng Bai, Ning Zhu, Jianhe Xu. Microfluidic reactor with immobilized enzyme-from construction to applications: A review[J]. 中国化学工程学报, 2021, 29(2): 136-145.

Han Zhang, Yunpeng Bai, Ning Zhu, Jianhe Xu. Microfluidic reactor with immobilized enzyme-from construction to applications: A review[J]. Chinese Journal of Chemical Engineering, 2021, 29(2): 136-145.

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