Self-sufficient Cytochrome P450s and their potential applications in biotechnology

doi:10.1016/j.cjche.2020.12.002

中国化学工程学报 ›› 2021, Vol. 29 ›› Issue (2): 121-135.DOI: 10.1016/j.cjche.2020.12.002

• Biocatalysis and Bioreactor Engineering • 上一篇下一篇

Self-sufficient Cytochrome P450s and their potential applications in biotechnology

Bekir Engin Eser¹, Yan Zhang¹, Li Zong^1,2, Zheng Guo¹

1 Department of Engineering, Faculty of Technical Sciences, Aarhus University, Denmark;
2 Key Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, School of Life Science, Jilin University, Changchun 130021, China

收稿日期:2020-10-14 修回日期:2020-12-07 出版日期:2021-02-28 发布日期:2021-05-15
通讯作者: Zheng Guo
基金资助:
Financial supports from Novo Nordisk Foundation (NNF16OC0021740), Aarhus Universitets Forskningsfond AUFFNOVA (AUFF-E-2015-FLS-9-12) and Danmarks Frie Forskningsfond (DFF Technology and Production, 0136-00206B) are greatly acknowledged.

Self-sufficient Cytochrome P450s and their potential applications in biotechnology

Bekir Engin Eser¹, Yan Zhang¹, Li Zong^1,2, Zheng Guo¹

1 Department of Engineering, Faculty of Technical Sciences, Aarhus University, Denmark;
2 Key Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, School of Life Science, Jilin University, Changchun 130021, China

Received:2020-10-14 Revised:2020-12-07 Online:2021-02-28 Published:2021-05-15
Contact: Zheng Guo
Supported by:
Financial supports from Novo Nordisk Foundation (NNF16OC0021740), Aarhus Universitets Forskningsfond AUFFNOVA (AUFF-E-2015-FLS-9-12) and Danmarks Frie Forskningsfond (DFF Technology and Production, 0136-00206B) are greatly acknowledged.

摘要/Abstract

摘要： Cytochrome P450s (CYPs) are ubiquitously found in all kingdoms of life, playing important role in various biosynthetic pathways as well as degradative pathways; accordingly find applications in a vast variety of areas from organic synthesis and drug metabolite production to modification of biomaterials and bioremediation. Significantly, CYPs catalyze chemically challenging C—H and C—C activation reactions using a reactive high-valent iron-oxo intermediate generated upon dioxygen activation at their heme center, while the other oxygen atom is reduced to the level of water by electrons provided through a reductase partner protein. Self-sufficient CYPs, encoding their heme domain and reductase protein in a single polypeptide, facilitate increased catalytic efficiency and render a less complicated system to work with. The self-sufficient CYP enzyme from CYP102A family (CYP102A1, BM3) is among the earliest and most-investigated model enzymes for mechanistic and structural studies as well as for biotechnological applications. An increasing number of self-sufficient CYPs from the same CYP102 family and from other families have also been reported in last decade. In this review, we introduce chemistry and biology of CYPs, followed by an overview of the characteristics of self-sufficient CYPs and representative reactions. Enzyme engineering efforts leading to novel self-sufficient CYP variants that can catalyze synthetically useful natural and non-natural (nature-mimicking) reactions are highlighted. Lastly, the strategy and efforts that aim to circumvent the challenges for improved thermostability, regio-and enantioselectivity, and total turnover number; associated with practical use of self-sufficient CYPs are reviewed.

关键词: Biocatalysis, Heme enzymes, C—H activation, Cytochrome P450s, Self-sufficient P450s, P450 BM3

Abstract: Cytochrome P450s (CYPs) are ubiquitously found in all kingdoms of life, playing important role in various biosynthetic pathways as well as degradative pathways; accordingly find applications in a vast variety of areas from organic synthesis and drug metabolite production to modification of biomaterials and bioremediation. Significantly, CYPs catalyze chemically challenging C—H and C—C activation reactions using a reactive high-valent iron-oxo intermediate generated upon dioxygen activation at their heme center, while the other oxygen atom is reduced to the level of water by electrons provided through a reductase partner protein. Self-sufficient CYPs, encoding their heme domain and reductase protein in a single polypeptide, facilitate increased catalytic efficiency and render a less complicated system to work with. The self-sufficient CYP enzyme from CYP102A family (CYP102A1, BM3) is among the earliest and most-investigated model enzymes for mechanistic and structural studies as well as for biotechnological applications. An increasing number of self-sufficient CYPs from the same CYP102 family and from other families have also been reported in last decade. In this review, we introduce chemistry and biology of CYPs, followed by an overview of the characteristics of self-sufficient CYPs and representative reactions. Enzyme engineering efforts leading to novel self-sufficient CYP variants that can catalyze synthetically useful natural and non-natural (nature-mimicking) reactions are highlighted. Lastly, the strategy and efforts that aim to circumvent the challenges for improved thermostability, regio-and enantioselectivity, and total turnover number; associated with practical use of self-sufficient CYPs are reviewed.

Key words: Biocatalysis, Heme enzymes, C—H activation, Cytochrome P450s, Self-sufficient P450s, P450 BM3

Bekir Engin Eser, Yan Zhang, Li Zong, Zheng Guo. Self-sufficient Cytochrome P450s and their potential applications in biotechnology[J]. 中国化学工程学报, 2021, 29(2): 121-135.

Bekir Engin Eser, Yan Zhang, Li Zong, Zheng Guo. Self-sufficient Cytochrome P450s and their potential applications in biotechnology[J]. Chinese Journal of Chemical Engineering, 2021, 29(2): 121-135.

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Self-sufficient Cytochrome P450s and their potential applications in biotechnology

Self-sufficient Cytochrome P450s and their potential applications in biotechnology

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