Immobilization of Penicillin G Acylase on Magnetic Nanoparticles Modified by Ionic Liquids

Chinese Journal of Chemical Engineering ›› 2012, Vol. 20 ›› Issue (1): 146-151.

Immobilization of Penicillin G Acylase on Magnetic Nanoparticles Modified by Ionic Liquids

周华从^1,2, 李伟¹, 寿庆辉^1,2, 高红帅¹, 徐芃^1,2, 邓伏礼^1,2, 刘会洲¹

1. State Key Laboratory of Biochemical Engineering, Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China;
2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China

收稿日期:2011-05-20 修回日期:2011-10-07 出版日期:2012-02-28 发布日期:2012-05-03
通讯作者: LI Wei,E-mail:wli@cnu.edu.cn;LIU Huizhou,E-mail:hzliu@home.ipe.ac.cn
基金资助:
Supported by the National Basic Research Program of China (2007CB613507)

Immobilization of Penicillin G Acylase on Magnetic Nanoparticles Modified by Ionic Liquids

ZHOU Huacong^1,2, LI Wei¹, SHOU Qinghui^1,2, GAO Hongshuai¹, XU Peng^1,2, DENG Fuli^1,2, LIU Huizhou¹

1. State Key Laboratory of Biochemical Engineering, Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China;
2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China

Received:2011-05-20 Revised:2011-10-07 Online:2012-02-28 Published:2012-05-03
Supported by:
Supported by the National Basic Research Program of China (2007CB613507)

摘要/Abstract

摘要： Functionalized ionic liquids containing ethyoxyl groups were synthesized and immobilized on magnetic silica nanoparticles(MSNP) prepared by two steps,i.e.,Fe₃O₄ synthesis and silica shell growth on the surface.This magnetic nanoparticle supported ionic liquid(MNP-IL) were applied in the immobilization of penicillin G acylase(PGA).The MSNPs and MNP-ILs were characterized by the means of Fourier transform infrared spectroscopy(FTIR),scanning electron microscopy(SEM),transmission electron microscopy(TEM),and vibrating sample magnetometer(VSM).The results showed that the average size of magnetic Fe₃O₄ nanoparticles and MSNPs were ~10 and ~90 nm,respectively.The saturation magnetizations of magnetic Fe₃O₄ nanoparticles and MNP-ILs were 63.7 and 26.9 A·m²·kg^-1,respectively.The MNP-IL was successfully applied in the immobilization of PGA.The maximum amount of loaded enzyme was about 209 mg·g^-1(based on carrier),and the highest enzyme activity of immobilized PGA(based on ImPGA) was 261 U·g^-1.Both the amount of loaded enzyme and the activity of ImPGA are at the same level of or higher than that in previous reports.After 10 consecutive operations,ImPGA still maintained 62% of its initial activity,indicating the good recovery property of ImPGA activity.The ionic liquid modified magnetic particles integrate the magnetic properties of Fe₃O₄ and the structure-tunable properties of ionic liquids,and have extensive potential uses in protein immobilization and magnetic bioseparation.This work may open up a novel strategy to immobilize proteins by ionic liquids.

关键词: magnetic iron oxide, magnetic nanoparticles, ionic liquids, penicillin G acylase, enzyme immobilization

Abstract: Functionalized ionic liquids containing ethyoxyl groups were synthesized and immobilized on magnetic silica nanoparticles(MSNP) prepared by two steps,i.e.,Fe₃O₄ synthesis and silica shell growth on the surface.This magnetic nanoparticle supported ionic liquid(MNP-IL) were applied in the immobilization of penicillin G acylase(PGA).The MSNPs and MNP-ILs were characterized by the means of Fourier transform infrared spectroscopy(FTIR),scanning electron microscopy(SEM),transmission electron microscopy(TEM),and vibrating sample magnetometer(VSM).The results showed that the average size of magnetic Fe₃O₄ nanoparticles and MSNPs were ~10 and ~90 nm,respectively.The saturation magnetizations of magnetic Fe₃O₄ nanoparticles and MNP-ILs were 63.7 and 26.9 A·m²·kg^-1,respectively.The MNP-IL was successfully applied in the immobilization of PGA.The maximum amount of loaded enzyme was about 209 mg·g^-1(based on carrier),and the highest enzyme activity of immobilized PGA(based on ImPGA) was 261 U·g^-1.Both the amount of loaded enzyme and the activity of ImPGA are at the same level of or higher than that in previous reports.After 10 consecutive operations,ImPGA still maintained 62% of its initial activity,indicating the good recovery property of ImPGA activity.The ionic liquid modified magnetic particles integrate the magnetic properties of Fe₃O₄ and the structure-tunable properties of ionic liquids,and have extensive potential uses in protein immobilization and magnetic bioseparation.This work may open up a novel strategy to immobilize proteins by ionic liquids.

Key words: magnetic iron oxide, magnetic nanoparticles, ionic liquids, penicillin G acylase, enzyme immobilization

中图分类号:

TQ925

周华从, 李伟, 寿庆辉, 高红帅, 徐芃, 邓伏礼, 刘会洲. Immobilization of Penicillin G Acylase on Magnetic Nanoparticles Modified by Ionic Liquids[J]. Chinese Journal of Chemical Engineering, 2012, 20(1): 146-151.

ZHOU Huacong, LI Wei, SHOU Qinghui, GAO Hongshuai, XU Peng, DENG Fuli, LIU Huizhou. Immobilization of Penicillin G Acylase on Magnetic Nanoparticles Modified by Ionic Liquids[J]. Chin.J.Chem.Eng., 2012, 20(1): 146-151.

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Immobilization of Penicillin G Acylase on Magnetic Nanoparticles Modified by Ionic Liquids

Immobilization of Penicillin G Acylase on Magnetic Nanoparticles Modified by Ionic Liquids

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