One Step Bioleaching of Sulphide Ore with Low Concentration of Arsenic by Aspergillus niger and Taguchi Orthogonal Array Optimization*

Chinese Journal of Chemical Engineering ›› 2012, Vol. 20 ›› Issue (5): 923-929.

One Step Bioleaching of Sulphide Ore with Low Concentration of Arsenic by Aspergillus niger and Taguchi Orthogonal Array Optimization^*

Sadia Ilyas^1,2, 池汝安¹, Jae Chun Lee², Haq Nawaz Bhatti³

1. Key Laboratory for Green Chemical Process of Ministry of Education, School of Chemical Engineering & Pharmacy, Wuhan Institute of Technology, Wuhan 430073, China;
2. Mineral Resoures Research Division, Korea Institute of Geoscience and Mineral Resources (KIGAM), 124 Gwahang-no, Yuseong-gu, Daejeon 305-350, Korea;
3. Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad 38040, Pakistan

收稿日期:2011-03-22 修回日期:2011-10-16 出版日期:2012-10-28 发布日期:2012-11-06
通讯作者: CHI Ru'an,E-mail:rac@mail.wit.edu.cn
基金资助:
Supported by the Program for Changjiang Scholars and Innovative Research Team in University, China (IRT0974);the Higher Education Commission of Pakistan (20-652/R&D/05-43622)

One Step Bioleaching of Sulphide Ore with Low Concentration of Arsenic by Aspergillus niger and Taguchi Orthogonal Array Optimization^*

Sadia Ilyas^1,2, CHI Ru'an¹, Jae Chun Lee², Haq Nawaz Bhatti³

1. Key Laboratory for Green Chemical Process of Ministry of Education, School of Chemical Engineering & Pharmacy, Wuhan Institute of Technology, Wuhan 430073, China;
2. Mineral Resoures Research Division, Korea Institute of Geoscience and Mineral Resources (KIGAM), 124 Gwahang-no, Yuseong-gu, Daejeon 305-350, Korea;
3. Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad 38040, Pakistan

Received:2011-03-22 Revised:2011-10-16 Online:2012-10-28 Published:2012-11-06
Supported by:
Supported by the Program for Changjiang Scholars and Innovative Research Team in University, China (IRT0974);the Higher Education Commission of Pakistan (20-652/R&D/05-43622)

摘要/Abstract

摘要： A study was carried out to examine the possibility for Aspergillus niger strain KBS4 to bioleach metals from sulphide ore with low concentration of arsenic and to optimize the parameters that affect this process by orthogonal array optimization. Fungal sample was collected, purified and sequenced. The bioleaching process was optimized with L25 Taguchi orthogonal experimental array design. Five factors were investigated and 25 batch bioleaching tests were run at five levels for each factor. The parameters were initial pH, particle size, pulp density, initial inoculums and residence time for bioleaching. The experimental results showed that under optimized leaching conditions: pH 5.5, particle size 180 μm, initial inoculums size 3×10⁷ spores per ml, pulp density 15% and residence time of 20 days, the bioleach ability of metals were 63% Fe, 68% Zn, 60% As, 79% Cu and 54% Al. The biosorption of metal ions by fungal biomass might occur during the bioleaching process but it did not hinder the removal of metal ions by bioleaching.

关键词: Aspergillus niger, bioleaching, sulphide ore, orthogonal array

Abstract: A study was carried out to examine the possibility for Aspergillus niger strain KBS4 to bioleach metals from sulphide ore with low concentration of arsenic and to optimize the parameters that affect this process by orthogonal array optimization. Fungal sample was collected, purified and sequenced. The bioleaching process was optimized with L25 Taguchi orthogonal experimental array design. Five factors were investigated and 25 batch bioleaching tests were run at five levels for each factor. The parameters were initial pH, particle size, pulp density, initial inoculums and residence time for bioleaching. The experimental results showed that under optimized leaching conditions: pH 5.5, particle size 180 μm, initial inoculums size 3×10⁷ spores per ml, pulp density 15% and residence time of 20 days, the bioleach ability of metals were 63% Fe, 68% Zn, 60% As, 79% Cu and 54% Al. The biosorption of metal ions by fungal biomass might occur during the bioleaching process but it did not hinder the removal of metal ions by bioleaching.

Key words: Aspergillus niger, bioleaching, sulphide ore, orthogonal array

Sadia Ilyas, 池汝安, Jae Chun Lee, Haq Nawaz Bhatti. One Step Bioleaching of Sulphide Ore with Low Concentration of Arsenic by Aspergillus niger and Taguchi Orthogonal Array Optimization^*[J]. Chinese Journal of Chemical Engineering, 2012, 20(5): 923-929.

Sadia Ilyas, CHI Ru'an, Jae Chun Lee, Haq Nawaz Bhatti. One Step Bioleaching of Sulphide Ore with Low Concentration of Arsenic by Aspergillus niger and Taguchi Orthogonal Array Optimization^*[J]. Chin.J.Chem.Eng., 2012, 20(5): 923-929.

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One Step Bioleaching of Sulphide Ore with Low Concentration of Arsenic by Aspergillus niger and Taguchi Orthogonal Array Optimization^*

One Step Bioleaching of Sulphide Ore with Low Concentration of Arsenic by Aspergillus niger and Taguchi Orthogonal Array Optimization^*

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