SCI和EI收录∣中国化工学会会刊

Chinese Journal of Chemical Engineering ›› 2012, Vol. 20 ›› Issue (5): 930-936.

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Optimizing the Chemical Compositions of Protective Agents for Freeze-drying Bifidobacterium longum BIOMA 5920*

杨婵媛1,2, 朱晓丽1,3, 范代娣1,2, 米钰1,2, 骆艳娥1,2, 惠俊峰1,2, 苏然1,2   

  1. 1. Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an 710069, China;
    2. Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710069, China;
    3. Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Urban and Environmental Science, Northwest University, Xi'an 710069, China
  • 收稿日期:2011-03-06 修回日期:2011-09-29 出版日期:2012-10-28 发布日期:2012-11-06
  • 通讯作者: FAN Daidi,E-mail:fandaidi@nwu.edu.cn;MI Yu,E-mail:mi_yu@nwu.edu.cn
  • 基金资助:
    Supported by the National High Technology Research and Development Program of China (2007AA03Z456);the National Natural Science Foundation of China (20776119, 21076169 and 31000019);the Xi’an Research and Development Program(NC08005, YF07078);the Scientific Research Program of Shaanxi Provincial Department of Education,China(08JK452,08JK453,JG08181,2010JC21,2010JS107,2010JS108, 2010JK876 and 2010JS109);Shaanxi Provincial Scientific Technology Research and Development Program (2007K06-03, 2010JQ2012, SJ08B03);the Specialized Research Fund for the Doctoral Program of Higher Education of China (20096101120023, 20096101110014);NWU Graduate Innovation and Creativity Funds (08YSY17);Shaanxi Key Subject Program, China

Optimizing the Chemical Compositions of Protective Agents for Freeze-drying Bifidobacterium longum BIOMA 5920*

YANG Chanyuan1,2, ZHU Xiaoli1,3, FAN Daidi1,2, MI Yu1,2, LUO Yan'e1,2, HUI Junfeng1,2, SU Ran1,2   

  1. 1. Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an 710069, China;
    2. Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710069, China;
    3. Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Urban and Environmental Science, Northwest University, Xi'an 710069, China
  • Received:2011-03-06 Revised:2011-09-29 Online:2012-10-28 Published:2012-11-06
  • Supported by:
    Supported by the National High Technology Research and Development Program of China (2007AA03Z456);the National Natural Science Foundation of China (20776119, 21076169 and 31000019);the Xi’an Research and Development Program(NC08005, YF07078);the Scientific Research Program of Shaanxi Provincial Department of Education,China(08JK452,08JK453,JG08181,2010JC21,2010JS107,2010JS108, 2010JK876 and 2010JS109);Shaanxi Provincial Scientific Technology Research and Development Program (2007K06-03, 2010JQ2012, SJ08B03);the Specialized Research Fund for the Doctoral Program of Higher Education of China (20096101120023, 20096101110014);NWU Graduate Innovation and Creativity Funds (08YSY17);Shaanxi Key Subject Program, China

摘要: Freeze drying has a deleterious effect on the viability of microorganisms. In front of this difficulty, the present study adopts response surface methodology to optimize the chemical compositions of protective agents to seek for maximum viability of Bifidobacterium longum BIOMA 5920 during freeze-drying. Through the comparative analysis of single protectant, the complex protective agents show better effect on the Bifidobacterium viability. Human-like collagen (HLC), trehalose and glycerol are confirmed as significant factors by Box-Behnken Design. The optimized formula for these three variables is tested as follows: HLC 1.23%, trehalose 11.50% and glycerol 4.65%. Under this formula, the viability is 88.23%, 39.67% higher in comparison to the control. The viable count is 1.07×109 cfu·g-1, greatly exceeding the minimum viable count requirement (106cfu·g-1).

关键词: Bifidobacterium, freeze-drying, viability, protective agent, response surface methodology

Abstract: Freeze drying has a deleterious effect on the viability of microorganisms. In front of this difficulty, the present study adopts response surface methodology to optimize the chemical compositions of protective agents to seek for maximum viability of Bifidobacterium longum BIOMA 5920 during freeze-drying. Through the comparative analysis of single protectant, the complex protective agents show better effect on the Bifidobacterium viability. Human-like collagen (HLC), trehalose and glycerol are confirmed as significant factors by Box-Behnken Design. The optimized formula for these three variables is tested as follows: HLC 1.23%, trehalose 11.50% and glycerol 4.65%. Under this formula, the viability is 88.23%, 39.67% higher in comparison to the control. The viable count is 1.07×109 cfu·g-1, greatly exceeding the minimum viable count requirement (106cfu·g-1).

Key words: Bifidobacterium, freeze-drying, viability, protective agent, response surface methodology