Improved 5-Aminolevulinic Acid Production with Recombinant Escherichia coli by a Short-term Dissolved Oxygen Shock in Fed-batch Fermentation

doi:10.1016/S1004-9541(13)60627-8

Chinese Journal of Chemical Engineering ›› 2013, Vol. 21 ›› Issue (11): 1291-1295.DOI: 10.1016/S1004-9541(13)60627-8

Improved 5-Aminolevulinic Acid Production with Recombinant Escherichia coli by a Short-term Dissolved Oxygen Shock in Fed-batch Fermentation

杨俊¹, 朱力¹, 傅维琦², 林逸君¹, 林建平¹, 岑沛霖¹

1 Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China;
2 Center for Systems Biology, University of Iceland, Reykjavik, Iceland

收稿日期:2012-06-04 修回日期:2013-01-14 出版日期:2013-11-28 发布日期:2013-11-26
通讯作者: LIN Jianping
基金资助:
Supported by the National Natural Science Foundation of China (20306026 and 20876141) and the National Basic Research Program of China (2007CB707805).

Improved 5-Aminolevulinic Acid Production with Recombinant Escherichia coli by a Short-term Dissolved Oxygen Shock in Fed-batch Fermentation

YANG Jun¹, ZHU Li¹, FU Weiqi², LIN Yijun¹, LIN Jianping¹, CEN Peilin¹

1 Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China;
2 Center for Systems Biology, University of Iceland, Reykjavik, Iceland

Received:2012-06-04 Revised:2013-01-14 Online:2013-11-28 Published:2013-11-26
Contact: LIN Jianping
Supported by:
Supported by the National Natural Science Foundation of China (20306026 and 20876141) and the National Basic Research Program of China (2007CB707805).

摘要/Abstract

摘要： 5-Aminolevulinic acid (ALA) is a common precursor for tetrapyrrole compounds in all kinds of organisms and has wide applications in agriculture and medicines. In this study, a new strategy, i.e. short-term dissolved oxygen (DO) shock during aerobic fermentation, was introduced to produce 5-aminolevulinic acid with a recombinant E. coli. Effects of duration time of DO shock operation on plasmid concentration, intracellular ALA synthase (ALAS) activity and ALA production were investigated in Erlenmeyer shake flasks. The results indicated that both ALAS activity and ALA yield were enhanced in an anaerobic operation of 45 min in the early exponential phase during fermentation, while they decreased when the anaerobic operation time was further increased to 60 min. The DO shock protocol was confirmed with the fed-batch fermentation in a 15 L fermenter and the ALA production achieved 9.4 g·L^-1 (72 mmol·L^-1), which is the highest yield in the fermentation broth reported up to now.

关键词: 5-aminolevulinic acid, synthase activity, dissolved oxygen shock, fed-batch fermentation, recombinant E. coli

Abstract: 5-Aminolevulinic acid (ALA) is a common precursor for tetrapyrrole compounds in all kinds of organisms and has wide applications in agriculture and medicines. In this study, a new strategy, i.e. short-term dissolved oxygen (DO) shock during aerobic fermentation, was introduced to produce 5-aminolevulinic acid with a recombinant E. coli. Effects of duration time of DO shock operation on plasmid concentration, intracellular ALA synthase (ALAS) activity and ALA production were investigated in Erlenmeyer shake flasks. The results indicated that both ALAS activity and ALA yield were enhanced in an anaerobic operation of 45 min in the early exponential phase during fermentation, while they decreased when the anaerobic operation time was further increased to 60 min. The DO shock protocol was confirmed with the fed-batch fermentation in a 15 L fermenter and the ALA production achieved 9.4 g·L^-1 (72 mmol·L^-1), which is the highest yield in the fermentation broth reported up to now.

Key words: 5-aminolevulinic acid, synthase activity, dissolved oxygen shock, fed-batch fermentation, recombinant E. coli

杨俊, 朱力, 傅维琦, 林逸君, 林建平, 岑沛霖. Improved 5-Aminolevulinic Acid Production with Recombinant Escherichia coli by a Short-term Dissolved Oxygen Shock in Fed-batch Fermentation[J]. Chinese Journal of Chemical Engineering, 2013, 21(11): 1291-1295.

YANG Jun, ZHU Li, FU Weiqi, LIN Yijun, LIN Jianping, CEN Peilin. Improved 5-Aminolevulinic Acid Production with Recombinant Escherichia coli by a Short-term Dissolved Oxygen Shock in Fed-batch Fermentation[J]. Chin.J.Chem.Eng., 2013, 21(11): 1291-1295.

参考文献

1 Sasaki, K., Watanabe, M., Tanaka, T., Tanaka, T., “Biosynthesis, biotechnological production and applications of 5-aminolevulinic acid”, Appl. Microbiol. Biotechnol., 58 (1), 23-29 (2002).

2 Kennedy, J.C., Pottier, R.H., Pross, D.C., “Photodynamic therapy with endogenous protoporphyrin IX: basic principles and present clinical experience”, J. Photoch. Photobio. B., 6, 143-148 (1990).

3 Sasikala, C.H., Ramana, V., Raghuveer, R.P., “5-Aminolevulinic acid: a potential herbicide/insecticide from micro-organism”, Biotechnol. Progr., 10 (5), 451-459 (1994).

4 Watanabe, K., Tanaka, T., Hotta, Y., Kuramochi, H., Takeuchi, Y., “Improving salt tolerance of cotton seedlings with 5-aminolevulinic acid”, Plant Growth Regul., 32 (1), 99-103 (2000).

5 Chen, H.M., Chen, C.T., Yang, H., Kuo, M., Kuo, Y.S., Lan, W.H., “Successful treatment of oral verrucous hyperplasia with topical 5-aminolevulinic acid-mediated photodynamic therapy”, Oral Oncol., 40 (6), 630-637 (2004).

6 Zhang, Z.J., Li, H.Z., Zhou, W.J., Takeuchi, Y., Yoneyama, K., “Effect of 5-aminolevulinic acid on development and salt tolerance of potato (Solanum tuberosum L.) microtubes in vitro”, Plant Growth Regul., 49 (1), 27-34 ( 2006).

7 Xie, L., Hall, D., Eiteman, M.A., Altman, E., “Optimization of recombinant aminolevulinate synthase production in Escherichia coli using factorial design”, Appl. Microbiol. Biotechnol., 63 (3), 267-273 (2003).

8 Chung, S.Y., Seo, K.H., Rhee, J.I., “Influence of culture conditions on the production of extra-cellular 5-aminolevulinic acid (ALA) by recombinant E-coli”, Process Biochem., 40 (1), 385-394 (2005).

9 Lee, D.H., Jun, W.J., Shin, D.H., Cho, H.Y., Hong, B.S., “Effect of culture conditions on production of 5-aminolevulinic acid by recombinant Escherichia coli”, Biosci. Biotech. Bioch., 69 (3), 470-476 (2005).

10 Qin, G., Lin, J.P., Liu, X.X., Cen, P.L., “Effects of medium composition on production of 5-aminolevulinic acid by recombinant Escherichia coli”, J. Biosci. Bioeng., 102 (4), 316-322 (2006).

11 Lin, J.P., Fu, W.Q., Cen, P.L., “Characterization of 5-aminolevulinate synthase from Agrobacterium radiobacter, screening new inhibitors for 5-aminolevulinate dehydratase from Escherichia coli and their potential use for high 5-aminolevulinate production”, Bioresource Technol., 100 (7), 2293-2297 (2009).

12 Sasaki, K., Watanabe, K., Tanaka, T., Hotta, Y., Nagai, S., “5-Aminolevulinic acid production by Chlorella SP during heterotrophic cultivation in the dark”, World J. Microb. Biot., 11 (3), 361-362 (1995).

13 Ano, A., Funahashi, H., Nakao, K., Nishizawa, Y., “Effect of glycine on 5-aminolevulinic acid biosynthesis in heterotrophic culture of Chlorella regularis YA-603”, J. Biosci. Bioeng., 88 (1), 57-60 (1999).

14 Noparatnaraporn, N., Watanabe, M., Sasaki, K., “Extracellular formation of 5-aminolevulinic acid by intact cells of the marine photosynthetic bacterium Rhodovulum sp under various pH conditions”, World J. Microb. Biot., 16 (3), 313-315 (2000).

15 Kamiyama, H., Hotta, Y., Tanaka, T., Nishikawa, S., Sasaki, K., “Production of 5-aminolevulinic acid by a mutant strain of a photosynthetic bacterium”, Seibutsu-Kogaku Kais., 78 (2), 48-55 (2000).

16 Nishikawa, S., Tanaka, T., Kaminaga, T., Miyachi, N., Watanabe, K., Hotta, Y., “Microorganisms producing 5-aminolevulinic acid and processing for producing 5-aminolevulinic acid by using the same”, U.S. Pat., 6342377 (2002).

17 De, Leon.A., Hernandez, V., Galindo, E., Ramirez, O.T., “Effects of dissolved oxygen tension on the production of recombinant penicillin acylase in Escherichia coli”, Enzyme Microb. Tech., 33 (5), 689-697 (2003).

18 Vemuri, G.N., Eiteman, M.A., Altman, E., “Succinate production in dual-phase Escherichia coli fermentations depends on the time of transition from aerobic to anaerobic conditions”, J. Ind. Microbiol. Biot., 28 (6), 325-332 (2002).

19 Liu, X.X., Lin, J.P., Qin, G., Cen, P.L., “Expression of a new gene from Agrobacterium radiobacter in Escherichia coli for 5-aminolevulinic acid production”, Chin. J. Chem. Eng., 13 (4), 522-528 (2005)

20 Fu, W.Q., Lin, J.P., Cen, P.L., “Enhancement of 5-aminolevulinate production with recombinant Escherichia coli using batch and fed-batch culture system”, Bioresource Technol., 99 (11), 4864-4870 (2008).

21 Burnham, B.F., “σ-Aminolevulinic acid synthase (from Rhodopseudomona spheroides)”, Method Enzymol., 17, 195-200 (1970).

22 Fan, L., Lin, J.P., Liu, X.X., Qin, G., Cen, P.L., “Determination of 5-aminolevulinic acid in E. coli fermentation broth by reversed phase high performance liquid chromatography with precolumn derivatization”, Chin. J. Anal. Chem., 34 (7), 937-940 (2006).

23 Fu, W.Q., Lin, J.P., Cen, P.L., “5-Aminolevulinate production with recombinant Escherichia coli using a rare codon optimizer host strain”, Appl. Microbiol. Biotechnol., 75 (4), 777-782 (2007).

24 Hellmuth, K., Korz, D.J., Sanders, E.A., Deckwer, W.D., “Effect of growth-rate on stability and gene-expression of recombinant plasmids during continuous and high cell-density cultivation of Escherichia coli TG1”, J. Biotechnol., 32 (3), 289-298 (1994).

25 Lee, D.H., Jun, W.J., Kim, K.M., Shin, D.H., Cho, H.Y., Hong, B.S., “Inhibition of 5-aminolevulinic acid dehydratase in recombinant Escherichia coli using D-glucose”, Enzyme Microb. Tech., 32 (1), 27-34 (2003).

[1]	Bo Lü, Xiaogang Yang, Xudong Feng, Chun Li. Enhanced production of glycyrrhetic acid 3-O-mono-β-D-glucuronide by fed-batch fermentation using pH and dissolved oxygen as feedback parameters[J]. Chinese Journal of Chemical Engineering, 2016, 24(4): 506-512.
[2]	Jiahuan Wu, Jianlin Wang, Tao Yu, Liqiang Zhao. An Approach to Continuous Approximation of Pareto Front Using Geometric Support Vector Regression for Multi-objective Optimization of Fermentation Process[J]. , 2014, 22(10): 1131-1140.
[3]	苗志奇, 元英进. Strategies for Optimizing Feed Rate of Fed-Batch Yeast Fermentation by Fuzzy-Neural Network[J]. , 1998, 6(4): 340-347.

Improved 5-Aminolevulinic Acid Production with Recombinant Escherichia coli by a Short-term Dissolved Oxygen Shock in Fed-batch Fermentation

Improved 5-Aminolevulinic Acid Production with Recombinant Escherichia coli by a Short-term Dissolved Oxygen Shock in Fed-batch Fermentation

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 3

编辑推荐

Metrics

本文评价