Combination of a crude oil-degrading bacterial consortium under the guidance of strain tolerance and a pilot-scale degradation test

doi:10.1016/j.cjche.2017.02.001

Chinese Journal of Chemical Engineering ›› 2017, Vol. 25 ›› Issue (12): 1838-1846.DOI: 10.1016/j.cjche.2017.02.001

• 第25届中国过程控制会议专栏 • 上一篇下一篇

Combination of a crude oil-degrading bacterial consortium under the guidance of strain tolerance and a pilot-scale degradation test

Yilin Liu¹, Chen Li¹, Lei Huang¹, Yun He¹, Tingting Zhao¹, Bo Han¹, Xiaoqiang Jia^1,2,3

1. Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China;
2. Key Laboratory of Systems Bioengineering (Tianjin University), Ministry of Education, Tianjin 300072, China;
3. Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300072, China

收稿日期:2016-11-04 修回日期:2017-01-30 出版日期:2017-12-28 发布日期:2018-01-18
通讯作者: Xiaoqiang Jia,E-mail address:xqjia@tju.edu.cn.
基金资助:
Supported by the National Basic Research Program of China (“973” Program: 2014CB745100), the National Natural Science Foundation of China (21576197), Tianjin Research Program of Application Foundation and Advanced Technology (14JCQNJC06700), the Major Research Plan of Tianjin (16YFXTSF00460) and Tianjin Penglai 19-3 Oil Spill Accident Compensation Project (19-3BC2014-03).

Combination of a crude oil-degrading bacterial consortium under the guidance of strain tolerance and a pilot-scale degradation test

Yilin Liu¹, Chen Li¹, Lei Huang¹, Yun He¹, Tingting Zhao¹, Bo Han¹, Xiaoqiang Jia^1,2,3

1. Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China;
2. Key Laboratory of Systems Bioengineering (Tianjin University), Ministry of Education, Tianjin 300072, China;
3. Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300072, China

Received:2016-11-04 Revised:2017-01-30 Online:2017-12-28 Published:2018-01-18
Contact: Xiaoqiang Jia,E-mail address:xqjia@tju.edu.cn.
Supported by:
Supported by the National Basic Research Program of China (“973” Program: 2014CB745100), the National Natural Science Foundation of China (21576197), Tianjin Research Program of Application Foundation and Advanced Technology (14JCQNJC06700), the Major Research Plan of Tianjin (16YFXTSF00460) and Tianjin Penglai 19-3 Oil Spill Accident Compensation Project (19-3BC2014-03).

摘要/Abstract

摘要： Under the guidance of strain tolerance, a new combinationmethod for crude oil-degrading bacterial consortium was studied. Firstly,more than 50 efficient crude oil-degrading and biosurfactant producing bacteriawere isolated from petroleum-contaminated soil andwater in Tianjin Binhai New Area Oilfield, China. Twenty-four of them were selected for further study. These strains were identified as belonging Pseudomonas aeruginosa, Bacillus subtilis, Brevibacillus brevis, Achromobacter sp., Acinetobacter venetianus, Lysinibacillus macroides, Klebsiella oxytoca, Stenotrophomonas rhizophila, Rhodococcus sp. and Bacillus thuringiensis. A shake-flask degradation test revealed that 12 of these strains could degrade over 50% of 1% crude oil concentration in 7 d. Of these, 8 strains were able to produce biosurfactants. Furthermore, environmental tolerance experiments indicated that the majority of the strains had the ability to adapt to extreme environments including high temperatures, alkaline environments and high salinity environments. A mixed bacterial agent comprising the strains WB2, W2, W3 and HA was developed based on the environmental tolerance tests and subjected to the pilot-scale degradation test indicating that this bacterial agent could degrade 85.2% of 0.8% crude oil concentration in 60 d. Our results suggest that the application of this mixed agent could remediate crude oil polluted soils in saline and alkaline environments.

关键词: Crude oil-degrading strains, Biosurfactant producer, Environmental tolerance, Pilot-scale bioremediation

Abstract: Under the guidance of strain tolerance, a new combinationmethod for crude oil-degrading bacterial consortium was studied. Firstly,more than 50 efficient crude oil-degrading and biosurfactant producing bacteriawere isolated from petroleum-contaminated soil andwater in Tianjin Binhai New Area Oilfield, China. Twenty-four of them were selected for further study. These strains were identified as belonging Pseudomonas aeruginosa, Bacillus subtilis, Brevibacillus brevis, Achromobacter sp., Acinetobacter venetianus, Lysinibacillus macroides, Klebsiella oxytoca, Stenotrophomonas rhizophila, Rhodococcus sp. and Bacillus thuringiensis. A shake-flask degradation test revealed that 12 of these strains could degrade over 50% of 1% crude oil concentration in 7 d. Of these, 8 strains were able to produce biosurfactants. Furthermore, environmental tolerance experiments indicated that the majority of the strains had the ability to adapt to extreme environments including high temperatures, alkaline environments and high salinity environments. A mixed bacterial agent comprising the strains WB2, W2, W3 and HA was developed based on the environmental tolerance tests and subjected to the pilot-scale degradation test indicating that this bacterial agent could degrade 85.2% of 0.8% crude oil concentration in 60 d. Our results suggest that the application of this mixed agent could remediate crude oil polluted soils in saline and alkaline environments.

Key words: Crude oil-degrading strains, Biosurfactant producer, Environmental tolerance, Pilot-scale bioremediation

Yilin Liu, Chen Li, Lei Huang, Yun He, Tingting Zhao, Bo Han, Xiaoqiang Jia. Combination of a crude oil-degrading bacterial consortium under the guidance of strain tolerance and a pilot-scale degradation test[J]. Chinese Journal of Chemical Engineering, 2017, 25(12): 1838-1846.

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Combination of a crude oil-degrading bacterial consortium under the guidance of strain tolerance and a pilot-scale degradation test

Combination of a crude oil-degrading bacterial consortium under the guidance of strain tolerance and a pilot-scale degradation test

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