Adsorption behaviors of avian immunoglobulins and purification of immunoglobulin Y from chicken serum with mixed-mode resins

doi:10.1016/j.cjche.2018.05.011

Chinese Journal of Chemical Engineering ›› 2019, Vol. 27 ›› Issue (3): 514-518.DOI: 10.1016/j.cjche.2018.05.011

• Separation Science and Engineering • 上一篇下一篇

Adsorption behaviors of avian immunoglobulins and purification of immunoglobulin Y from chicken serum with mixed-mode resins

Yingdi Luo, Qilei Zhang, Shanjing Yao, Dongqiang Lin

Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China

收稿日期:2018-02-13 修回日期:2018-05-07 出版日期:2019-03-28 发布日期:2019-04-25
通讯作者: Dongqiang Lin,E-mail address:lindq@zju.edu.cn
基金资助:
Supported by the National Natural Science Foundation of China (21776243,21476198) and the International Science&Technology Cooperation Program of China (2015DFG42070).

Adsorption behaviors of avian immunoglobulins and purification of immunoglobulin Y from chicken serum with mixed-mode resins

Yingdi Luo, Qilei Zhang, Shanjing Yao, Dongqiang Lin

Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China

Received:2018-02-13 Revised:2018-05-07 Online:2019-03-28 Published:2019-04-25
Contact: Dongqiang Lin,E-mail address:lindq@zju.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China (21776243,21476198) and the International Science&Technology Cooperation Program of China (2015DFG42070).

摘要/Abstract

摘要： The importance of immunoglobulin Y (IgY) as a specific antibody equivalent to mammalian immunoglobulin G (IgG) is well recognized. However, production of highly purified IgY is still difficult due to the lack of specific purification methods. In this study, adsorption behaviors of IgY on four mixed-mode resins with functional ligands of 4-mercatoethyl-pyridine (MEP), 2-mercapto-1-methyli-midazole (MMI), 5-aminobenzi-midazole (ABI) and tryptophan-5-aminobenzi-midazole (W-ABI) were evaluated. The results showed that high adsorption ratio were found at pH 6.0-7.0 with little adsorption under acidic conditions. The resin with ABI ligand was then used to separate IgY from immunized chicken serum. An efficient process with IgY purity of 95% and recovery of 90% was developed after optimization of loading and elution pH and injection volume. The biological activity of the purified IgY was fully maintained. These results indicated that mixed-mode chromatography with specially-designed ligands has great potential for the separation of IgY from crude feedstock.

关键词: Mixed-mode chromatography, Immunoglobulin Y, Adsorption selectivity, Separation

Abstract: The importance of immunoglobulin Y (IgY) as a specific antibody equivalent to mammalian immunoglobulin G (IgG) is well recognized. However, production of highly purified IgY is still difficult due to the lack of specific purification methods. In this study, adsorption behaviors of IgY on four mixed-mode resins with functional ligands of 4-mercatoethyl-pyridine (MEP), 2-mercapto-1-methyli-midazole (MMI), 5-aminobenzi-midazole (ABI) and tryptophan-5-aminobenzi-midazole (W-ABI) were evaluated. The results showed that high adsorption ratio were found at pH 6.0-7.0 with little adsorption under acidic conditions. The resin with ABI ligand was then used to separate IgY from immunized chicken serum. An efficient process with IgY purity of 95% and recovery of 90% was developed after optimization of loading and elution pH and injection volume. The biological activity of the purified IgY was fully maintained. These results indicated that mixed-mode chromatography with specially-designed ligands has great potential for the separation of IgY from crude feedstock.

Key words: Mixed-mode chromatography, Immunoglobulin Y, Adsorption selectivity, Separation

Yingdi Luo, Qilei Zhang, Shanjing Yao, Dongqiang Lin. Adsorption behaviors of avian immunoglobulins and purification of immunoglobulin Y from chicken serum with mixed-mode resins[J]. Chinese Journal of Chemical Engineering, 2019, 27(3): 514-518.

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Adsorption behaviors of avian immunoglobulins and purification of immunoglobulin Y from chicken serum with mixed-mode resins

Adsorption behaviors of avian immunoglobulins and purification of immunoglobulin Y from chicken serum with mixed-mode resins

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