In situ investigation of lysozyme adsorption into polyelectrolyte brushes by quartz crystal microbalance with dissipation

doi:10.1016/j.cjche.2021.04.016

中国化学工程学报 ›› 2022, Vol. 48 ›› Issue (8): 106-115.DOI: 10.1016/j.cjche.2021.04.016

In situ investigation of lysozyme adsorption into polyelectrolyte brushes by quartz crystal microbalance with dissipation

Fenfen You¹, Qing-Hong Shi^1,2

1. Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, China;
2. Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin 300072, China

收稿日期:2021-02-21 修回日期:2021-04-23 出版日期:2022-08-28 发布日期:2022-09-30
通讯作者: Qing-Hong Shi,E-mail:qhshi@tju.edu.cn
基金资助:
This work was supported by the National Natural Science Foundation of China (Nos. 21878221 and 21476166), and the Foundation for Innovative Research Groups of the National Natural Science Foundation of China (No. 21621004).

In situ investigation of lysozyme adsorption into polyelectrolyte brushes by quartz crystal microbalance with dissipation

Fenfen You¹, Qing-Hong Shi^1,2

1. Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, China;
2. Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin 300072, China

Received:2021-02-21 Revised:2021-04-23 Online:2022-08-28 Published:2022-09-30
Contact: Qing-Hong Shi,E-mail:qhshi@tju.edu.cn
Supported by:
This work was supported by the National Natural Science Foundation of China (Nos. 21878221 and 21476166), and the Foundation for Innovative Research Groups of the National Natural Science Foundation of China (No. 21621004).

摘要/Abstract

摘要： A well understanding about protein adsorption into charged polymer brushes is of importance in the elucidation of mechanism and important phenomena (such as “chain delivery” effect) in protein adsorption on polymer-grafted ion exchange adsorbents. In this work, quartz crystal microbalance with dissipation (QCM-D) was introduced to in situ investigate lysozyme adsorption on QCM sensors grafted with poly(3-sulfopropyl methacrylate) (pSPM) via atom transfer radical polymerization. It was achieved by analyzing frequency (f) and energy dissipation (D) shift simultaneously on pSPM-grafted sensors. The result showed that an initial decrease in ΔD was typical of lysozyme adsorption on pSPM-grafted sensor and more significant with an increase of chain length and grafting density. It was attributed to significant water release in the hydration layer of protein and polymer chains in lysozyme adsorption into pSPM brushes. On pSPM-grafted sensors with long and dense chains, furthermore, lysozyme transitioned from monolayer to multilayer adsorption and the maximum adsorbed amount was obtained to be 374.0?ng·cm^?2 among all pSPM-grafted sensors in this work. The results in D-f plot further revealed that lysozyme adsorption into pSPM brushes increased the rigidity of adsorbed layer and little structure adjustment of adsorbed lysozyme. It was unfavorable for “chain delivery” effect for facilitated transport of adsorbed protein. This work provided valuable insight into protein adsorption in pSPM brushes and outlined a feasible approach to increasing mass transport in polymer-grafted ion exchange adsorbents.

关键词: Adsorption, Polymers, Protein, Water release, Graft density, Chain length

Abstract: A well understanding about protein adsorption into charged polymer brushes is of importance in the elucidation of mechanism and important phenomena (such as “chain delivery” effect) in protein adsorption on polymer-grafted ion exchange adsorbents. In this work, quartz crystal microbalance with dissipation (QCM-D) was introduced to in situ investigate lysozyme adsorption on QCM sensors grafted with poly(3-sulfopropyl methacrylate) (pSPM) via atom transfer radical polymerization. It was achieved by analyzing frequency (f) and energy dissipation (D) shift simultaneously on pSPM-grafted sensors. The result showed that an initial decrease in ΔD was typical of lysozyme adsorption on pSPM-grafted sensor and more significant with an increase of chain length and grafting density. It was attributed to significant water release in the hydration layer of protein and polymer chains in lysozyme adsorption into pSPM brushes. On pSPM-grafted sensors with long and dense chains, furthermore, lysozyme transitioned from monolayer to multilayer adsorption and the maximum adsorbed amount was obtained to be 374.0?ng·cm^?2 among all pSPM-grafted sensors in this work. The results in D-f plot further revealed that lysozyme adsorption into pSPM brushes increased the rigidity of adsorbed layer and little structure adjustment of adsorbed lysozyme. It was unfavorable for “chain delivery” effect for facilitated transport of adsorbed protein. This work provided valuable insight into protein adsorption in pSPM brushes and outlined a feasible approach to increasing mass transport in polymer-grafted ion exchange adsorbents.

Key words: Adsorption, Polymers, Protein, Water release, Graft density, Chain length

Fenfen You, Qing-Hong Shi. In situ investigation of lysozyme adsorption into polyelectrolyte brushes by quartz crystal microbalance with dissipation[J]. 中国化学工程学报, 2022, 48(8): 106-115.

Fenfen You, Qing-Hong Shi. In situ investigation of lysozyme adsorption into polyelectrolyte brushes by quartz crystal microbalance with dissipation[J]. Chinese Journal of Chemical Engineering, 2022, 48(8): 106-115.

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In situ investigation of lysozyme adsorption into polyelectrolyte brushes by quartz crystal microbalance with dissipation

In situ investigation of lysozyme adsorption into polyelectrolyte brushes by quartz crystal microbalance with dissipation

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