Development of an integrated process for the production of high-purity γ-aminobutyric acid from fermentation broth

doi:10.1016/j.cjche.2022.06.012

中国化学工程学报 ›› 2022, Vol. 50 ›› Issue (10): 361-368.DOI: 10.1016/j.cjche.2022.06.012

• Biotechnology and Bioengineering • 上一篇下一篇

Development of an integrated process for the production of high-purity γ-aminobutyric acid from fermentation broth

Zhaofeng Zhang¹, Juanjuan Ding¹, Min Wu¹, Bochao Liu⁵, Huiwen Song⁵, Shengping You^1,4, Wei Qi^1,2,3,4, Rongxin Su^1,2,3,4, Zhimin He^1,2

1 Chemical Engineering Research Center, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China;
2 State Key Laboratory of Chemical Engineering, Tianjin University, Tianjin 300072, China;
3 Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300072, China;
4 Tianjin Key Laboratory of Membrane Science and Desalination Technology, Tianjin University, Tianjin 300072, China;
5 Henan Julong Biological Engineering Co., Ltd, China

收稿日期:2022-03-17 修回日期:2022-05-05 出版日期:2022-10-28 发布日期:2023-01-04
通讯作者: Shengping You,E-mail:ysp@tju.edu.cn;Wei Qi,E-mail:qiwei@tju.edu.cn;Zhimin He,E-mail:enzyme@tju.edu.cn
基金资助:
This work was supported by the National Natural Science Foundation of China (Nos. 21621004, 22078239), the Beijing-Tianjin-Hebei Basic Research Cooperation Project (B2021210008), the Tianjin Development Program for Innovation and Entrepreneurship (2018).

Development of an integrated process for the production of high-purity γ-aminobutyric acid from fermentation broth

Zhaofeng Zhang¹, Juanjuan Ding¹, Min Wu¹, Bochao Liu⁵, Huiwen Song⁵, Shengping You^1,4, Wei Qi^1,2,3,4, Rongxin Su^1,2,3,4, Zhimin He^1,2

1 Chemical Engineering Research Center, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China;
2 State Key Laboratory of Chemical Engineering, Tianjin University, Tianjin 300072, China;
3 Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300072, China;
4 Tianjin Key Laboratory of Membrane Science and Desalination Technology, Tianjin University, Tianjin 300072, China;
5 Henan Julong Biological Engineering Co., Ltd, China

Received:2022-03-17 Revised:2022-05-05 Online:2022-10-28 Published:2023-01-04
Contact: Shengping You,E-mail:ysp@tju.edu.cn;Wei Qi,E-mail:qiwei@tju.edu.cn;Zhimin He,E-mail:enzyme@tju.edu.cn
Supported by:
This work was supported by the National Natural Science Foundation of China (Nos. 21621004, 22078239), the Beijing-Tianjin-Hebei Basic Research Cooperation Project (B2021210008), the Tianjin Development Program for Innovation and Entrepreneurship (2018).

摘要/Abstract

摘要： γ-Aminobutyric acid (GABA), a natural non-protein amino acid, plays an irreplaceable role in regulating the life activities of organisms. Nowadays, the separation and purification of food-grade GABA from fermentation broth is still a great challenge. This research utilized monosodium glutamate as a substrate for the production of high-purity GABA via an integrated process incorporating fermentation, purification, and crystallization. Firstly, 147 g·L^-1 GABA with a yield of 99.8% was achieved through fed-batch fermentation by Lactobacillus brevis CE701. Secondly, three integrated purification methods by ethanol precipitation were compared, and crude GABA with a purity of 89.85% was obtained by the optimized method. Thirdly, GABA crystals with a purity of 98.69% and a yield of 60% were further obtained through a designed crystallization process. Furthermore, the GABA industrial production process model was established by Superproper Designer V10 software, and material balance and economic analysis were carried out. Ethanol used in the process was recovered with a recovery of 98.79% through Aspen simulated extractive distillation. Then the fixed investment (equipment purchase and installation costs) for an annual production of 80 t GABA will be about 833000 USD; the total annual production cost (raw material cost and utility cost) will be about 641000 USD. The annual sale of GABA may be at the range of 2400000-4000000 USD and the payback period will be about 1-2 year. This integrated process provides a potential way for the industrial-scale production of food-grade GABA.

关键词: Fermentation, Desalination, Precipitation, Crystallization, Superproper Designer V10, Aspen

Abstract: γ-Aminobutyric acid (GABA), a natural non-protein amino acid, plays an irreplaceable role in regulating the life activities of organisms. Nowadays, the separation and purification of food-grade GABA from fermentation broth is still a great challenge. This research utilized monosodium glutamate as a substrate for the production of high-purity GABA via an integrated process incorporating fermentation, purification, and crystallization. Firstly, 147 g·L^-1 GABA with a yield of 99.8% was achieved through fed-batch fermentation by Lactobacillus brevis CE701. Secondly, three integrated purification methods by ethanol precipitation were compared, and crude GABA with a purity of 89.85% was obtained by the optimized method. Thirdly, GABA crystals with a purity of 98.69% and a yield of 60% were further obtained through a designed crystallization process. Furthermore, the GABA industrial production process model was established by Superproper Designer V10 software, and material balance and economic analysis were carried out. Ethanol used in the process was recovered with a recovery of 98.79% through Aspen simulated extractive distillation. Then the fixed investment (equipment purchase and installation costs) for an annual production of 80 t GABA will be about 833000 USD; the total annual production cost (raw material cost and utility cost) will be about 641000 USD. The annual sale of GABA may be at the range of 2400000-4000000 USD and the payback period will be about 1-2 year. This integrated process provides a potential way for the industrial-scale production of food-grade GABA.

Key words: Fermentation, Desalination, Precipitation, Crystallization, Superproper Designer V10, Aspen

Zhaofeng Zhang, Juanjuan Ding, Min Wu, Bochao Liu, Huiwen Song, Shengping You, Wei Qi, Rongxin Su, Zhimin He. Development of an integrated process for the production of high-purity γ-aminobutyric acid from fermentation broth[J]. 中国化学工程学报, 2022, 50(10): 361-368.

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Development of an integrated process for the production of high-purity γ-aminobutyric acid from fermentation broth

Development of an integrated process for the production of high-purity γ-aminobutyric acid from fermentation broth

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