Preparation and Characterization of Biological Non-toxic Hybrid Nanoparticles Based on Lactide and Poly(ethylene glycol) Loading Docetaxel for Anticancer Drug Delivery

doi:10.1016/j.cjche.2014.09.012

›› 2014, Vol. 22 ›› Issue (11/12): 1357-1362.DOI: 10.1016/j.cjche.2014.09.012

Preparation and Characterization of Biological Non-toxic Hybrid Nanoparticles Based on Lactide and Poly(ethylene glycol) Loading Docetaxel for Anticancer Drug Delivery

Ruiduan Li, Jianjun Zhang, Jianfeng Chen, Wei Teng, Jiexin Wang, Caixia Li

State Key Laboratory of Origanic-inorganic Composites, Beijing University of Chemical Technology, Beijing 100029, China

收稿日期:2013-12-09 修回日期:2014-02-13 出版日期:2014-12-28 发布日期:2014-12-24
通讯作者: Jianjun Zhang, Jianfeng Chen
基金资助:
Supported by the National Natural Science Foundation of China (51303009) and the NationalHigh Technology Research andDevelopment Programof China (2013AA032201).

Preparation and Characterization of Biological Non-toxic Hybrid Nanoparticles Based on Lactide and Poly(ethylene glycol) Loading Docetaxel for Anticancer Drug Delivery

Ruiduan Li, Jianjun Zhang, Jianfeng Chen, Wei Teng, Jiexin Wang, Caixia Li

State Key Laboratory of Origanic-inorganic Composites, Beijing University of Chemical Technology, Beijing 100029, China

Received:2013-12-09 Revised:2014-02-13 Online:2014-12-28 Published:2014-12-24
Supported by:
Supported by the National Natural Science Foundation of China (51303009) and the NationalHigh Technology Research andDevelopment Programof China (2013AA032201).

摘要/Abstract

摘要： Hybrid nanoparticles based on lactide and poly(ethylene glycol) were composed of a copolymer, poly(2- amino,1,3-propanediol carbonic ester-co-lactide) [P(LA-co-CA)], and a graft copolymer, poly(2-amino,1,3- propanediol carbonic ester-co-L-lactide)-g-methoxy-poly(ethylene glycol) [P(LA-co-CA)-mPEG]. The hybrid nanoparticles were prepared using emulsion solvent diffusion method. The copolymer of poly(2-benzyloxy amide,1,3-propanediol carbonic ester-co-lactide) was prepared using ring-opening polymerization with diethylzinc (ZnEt₂) as a catalyst, and then took off benzyl oxygen group to obtain P(LA-co-CA). P(LA-co-CA)-mPEG by grafting methoxy-PEG-propionaldehyde (mPEG-ALD) on P(LA-co-CA). With docetaxel as a model drug, the morphology of nanoparticles was characterized by scanning electron microscopy (SEM) and the size and size distributionwere determined by dynamic light scattering (DLS). The size of DTX-loaded particle was approximately 110 nm. The size scale prevents themfrom uptake by the reticulo-endothelial system and accumulation at the target site through the enhanced permeability and retention effect. InMTT assay, the results showed that the polymers are non-cytotoxic. The study points to the potential application of the composite nanoparticles in biomedical applications, including tissue engineering and controlled drug delivery.

关键词: Polylactide, Polycarbonate, Nanoparticles, Copolymer

Abstract: Hybrid nanoparticles based on lactide and poly(ethylene glycol) were composed of a copolymer, poly(2- amino,1,3-propanediol carbonic ester-co-lactide) [P(LA-co-CA)], and a graft copolymer, poly(2-amino,1,3- propanediol carbonic ester-co-L-lactide)-g-methoxy-poly(ethylene glycol) [P(LA-co-CA)-mPEG]. The hybrid nanoparticles were prepared using emulsion solvent diffusion method. The copolymer of poly(2-benzyloxy amide,1,3-propanediol carbonic ester-co-lactide) was prepared using ring-opening polymerization with diethylzinc (ZnEt₂) as a catalyst, and then took off benzyl oxygen group to obtain P(LA-co-CA). P(LA-co-CA)-mPEG by grafting methoxy-PEG-propionaldehyde (mPEG-ALD) on P(LA-co-CA). With docetaxel as a model drug, the morphology of nanoparticles was characterized by scanning electron microscopy (SEM) and the size and size distributionwere determined by dynamic light scattering (DLS). The size of DTX-loaded particle was approximately 110 nm. The size scale prevents themfrom uptake by the reticulo-endothelial system and accumulation at the target site through the enhanced permeability and retention effect. InMTT assay, the results showed that the polymers are non-cytotoxic. The study points to the potential application of the composite nanoparticles in biomedical applications, including tissue engineering and controlled drug delivery.

Key words: Polylactide, Polycarbonate, Nanoparticles, Copolymer

Ruiduan Li, Jianjun Zhang, Jianfeng Chen, Wei Teng, Jiexin Wang, Caixia Li. Preparation and Characterization of Biological Non-toxic Hybrid Nanoparticles Based on Lactide and Poly(ethylene glycol) Loading Docetaxel for Anticancer Drug Delivery[J]. , 2014, 22(11/12): 1357-1362.

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Preparation and Characterization of Biological Non-toxic Hybrid Nanoparticles Based on Lactide and Poly(ethylene glycol) Loading Docetaxel for Anticancer Drug Delivery

Preparation and Characterization of Biological Non-toxic Hybrid Nanoparticles Based on Lactide and Poly(ethylene glycol) Loading Docetaxel for Anticancer Drug Delivery

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