Engineering organoid microfluidic system for biomedical and health engineering: A review

doi:10.1016/j.cjche.2020.11.013

中国化学工程学报 ›› 2021, Vol. 29 ›› Issue (2): 244-254.DOI: 10.1016/j.cjche.2020.11.013

• Biomedical Engineering • 上一篇下一篇

Engineering organoid microfluidic system for biomedical and health engineering: A review

Yifan Xing¹, Junyu Liu¹, Xiaojie Guo¹, Haipeng Liu¹, Wen Zeng¹, Yi Wang¹, Chong Zhang^1,2, Yuan Lu¹, Dong He³, Shaohua Ma⁴, Yonghong He⁴, Xin-Hui Xing^1,2,4,5

1 Key Laboratory for Industrial Biocatalysis, Ministry of Education of China, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China;
2 Centre for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China;
3 School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China;
4 Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Shenzhen 518055, China;
5 Institute of Biomedical Health Technology and Engineering, Shenzhen Bay Laboratory, Shenzhen 518055, China

收稿日期:2020-10-22 修回日期:2020-11-24 出版日期:2021-02-28 发布日期:2021-05-15
通讯作者: Xin-Hui Xing
基金资助:
Y.F. Xing and J.Y. Liu contributed equally to this work. The authors thank the Key Laboratory for Industrial Biocatalysis, Ministry of Education of China, Department of Chemical Engineering, Tsinghua University, Beijing, China. This work was supported by the Key Areas Research Development Projects of Guangdong Province (No. 2019B020210001), the Tsinghua-U Tokyo Collaborative Research Fund (No. 20193080052), and the Key Areas Research Development Projects of Hebei Province (No.20375502D).

Engineering organoid microfluidic system for biomedical and health engineering: A review

Yifan Xing¹, Junyu Liu¹, Xiaojie Guo¹, Haipeng Liu¹, Wen Zeng¹, Yi Wang¹, Chong Zhang^1,2, Yuan Lu¹, Dong He³, Shaohua Ma⁴, Yonghong He⁴, Xin-Hui Xing^1,2,4,5

1 Key Laboratory for Industrial Biocatalysis, Ministry of Education of China, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China;
2 Centre for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China;
3 School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China;
4 Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Shenzhen 518055, China;
5 Institute of Biomedical Health Technology and Engineering, Shenzhen Bay Laboratory, Shenzhen 518055, China

Received:2020-10-22 Revised:2020-11-24 Online:2021-02-28 Published:2021-05-15
Contact: Xin-Hui Xing
Supported by:
Y.F. Xing and J.Y. Liu contributed equally to this work. The authors thank the Key Laboratory for Industrial Biocatalysis, Ministry of Education of China, Department of Chemical Engineering, Tsinghua University, Beijing, China. This work was supported by the Key Areas Research Development Projects of Guangdong Province (No. 2019B020210001), the Tsinghua-U Tokyo Collaborative Research Fund (No. 20193080052), and the Key Areas Research Development Projects of Hebei Province (No.20375502D).

摘要/Abstract

摘要： In recent years, organoid technology, i.e., in vitro three-dimensional (3D) tissue culture, has attracted increasing attention in biomedical engineering. Organoids are cell complexes induced by differentiation of stem cells or organ-progenitor cells in vitro using 3D culture technology. They can replicate the key structural and functional characteristics of the target organs in vivo. With the opening up of this new field of health engineering, there is a need for engineering-system approaches to the production, control, and quantitative analysis of organoids and their microenvironment. Traditional organoid technology has limitations, including lack of physical and chemical microenvironment control, high heterogeneity, complex manual operation, imperfect nutritional supply system, and lack of feasible online analytical technology for the organoids. The introduction of microfluidic chip technology into organoids has overcome many of these limitations and greatly expanded the scope of applications. Engineering organoid microfluidic system has become an interdisciplinary field in biomedical and health engineering. In this review, we summarize the development and culture system of organoids, discuss how microfluidic technology has been used to solve the main technical challenges in organoid research and development, and point out new opportunities and prospects for applications of organoid microfluidic system in drug development and screening, food safety, precision medicine, and other biomedical and health engineering fields.

关键词: Organoids, Stem cell, Culture system, Microfluidics, Biomedical engineering, Human health

Abstract: In recent years, organoid technology, i.e., in vitro three-dimensional (3D) tissue culture, has attracted increasing attention in biomedical engineering. Organoids are cell complexes induced by differentiation of stem cells or organ-progenitor cells in vitro using 3D culture technology. They can replicate the key structural and functional characteristics of the target organs in vivo. With the opening up of this new field of health engineering, there is a need for engineering-system approaches to the production, control, and quantitative analysis of organoids and their microenvironment. Traditional organoid technology has limitations, including lack of physical and chemical microenvironment control, high heterogeneity, complex manual operation, imperfect nutritional supply system, and lack of feasible online analytical technology for the organoids. The introduction of microfluidic chip technology into organoids has overcome many of these limitations and greatly expanded the scope of applications. Engineering organoid microfluidic system has become an interdisciplinary field in biomedical and health engineering. In this review, we summarize the development and culture system of organoids, discuss how microfluidic technology has been used to solve the main technical challenges in organoid research and development, and point out new opportunities and prospects for applications of organoid microfluidic system in drug development and screening, food safety, precision medicine, and other biomedical and health engineering fields.

Key words: Organoids, Stem cell, Culture system, Microfluidics, Biomedical engineering, Human health

Yifan Xing, Junyu Liu, Xiaojie Guo, Haipeng Liu, Wen Zeng, Yi Wang, Chong Zhang, Yuan Lu, Dong He, Shaohua Ma, Yonghong He, Xin-Hui Xing. Engineering organoid microfluidic system for biomedical and health engineering: A review[J]. 中国化学工程学报, 2021, 29(2): 244-254.

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Engineering organoid microfluidic system for biomedical and health engineering: A review

Engineering organoid microfluidic system for biomedical and health engineering: A review

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