Characterisation and separation of infectious bursal disease virus-like particles using aqueous two-phase systems

doi:10.1016/j.cjche.2022.08.019

中国化学工程学报 ›› 2023, Vol. 57 ›› Issue (5): 72-78.DOI: 10.1016/j.cjche.2022.08.019

• Full Length Article • 上一篇下一篇

Characterisation and separation of infectious bursal disease virus-like particles using aqueous two-phase systems

Hui Yi Leong¹, Xiao-Qian Fu¹, Xiang-Yu Liu², Shan-Jing Yao¹, Dong-Qiang Lin¹

1. Zhejiang Key Laboratory of Smart Biomaterials, Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China;
2. Sinopharm Animal Health Corporation Ltd., Wuhan 430075, China

收稿日期:2022-07-06 修回日期:2022-08-16 出版日期:2023-05-28 发布日期:2023-07-08
通讯作者: Dong-Qiang Lin,E-mail:lindq@zju.edu.cn
基金资助:
The authors would like to thank Zhejiang University and Talent-Introduction Program of China for Postdoctoral Researcher for the financial support. This work was financially supported by the National Key Research & Development Program of China (2021YFE0113300); and the National Natural Science Foundation of China (22078286).

Characterisation and separation of infectious bursal disease virus-like particles using aqueous two-phase systems

Hui Yi Leong¹, Xiao-Qian Fu¹, Xiang-Yu Liu², Shan-Jing Yao¹, Dong-Qiang Lin¹

1. Zhejiang Key Laboratory of Smart Biomaterials, Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China;
2. Sinopharm Animal Health Corporation Ltd., Wuhan 430075, China

Received:2022-07-06 Revised:2022-08-16 Online:2023-05-28 Published:2023-07-08
Contact: Dong-Qiang Lin,E-mail:lindq@zju.edu.cn
Supported by:
The authors would like to thank Zhejiang University and Talent-Introduction Program of China for Postdoctoral Researcher for the financial support. This work was financially supported by the National Key Research & Development Program of China (2021YFE0113300); and the National Natural Science Foundation of China (22078286).

摘要/Abstract

摘要： Infectious bursal disease (IBD) causes considerable economic losses in the commercial poultry industry worldwide. The principal way to control IBD virus (IBDV), the causative agent of IBD, is still through vaccination programs. Virus-like particles (VLPs) are recognised as a safe and potent recombinant vaccine platform. This research work explores the characterisation and separation of infectious bursal disease virus-like particles (IBD-VLPs) from crude feedstock. Various characteristics were studied with high-performance size-exclusion chromatography (HP-SEC), sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE) and transmission electron microscopy (TEM) analyses. Subsequently, the separation of IBD-VLPs using polyethylene glycol (PEG)/sodium citrate-based aqueous two-phase systems (ATPSs) was conducted and optimised. Moreover, a scale-up study of the best ATPS constituted of 15% PEG 6000, 11% sodium citrate and 10% crude feedstock was performed to compare the separation performance of IBD-VLPs with and without centrifugation-assisted. The results indicated that the optimised ATPS with centrifugation-assisted for both 5 g and 50 g systems showed good recovery of IBD-VLPs of >97% in the interphase between the PEG-rich top and salt-rich bottom phases. These optimised systems also showed high removal efficiencies of impurities of >95%. The results demonstrated that aqueous two-phase extraction could be a promising technology for efficient VLPs separation.

关键词: Aqueous two-phase extraction, Infectious bursal disease virus, Polymers, Salt, Separation, Virus-like particle

Abstract: Infectious bursal disease (IBD) causes considerable economic losses in the commercial poultry industry worldwide. The principal way to control IBD virus (IBDV), the causative agent of IBD, is still through vaccination programs. Virus-like particles (VLPs) are recognised as a safe and potent recombinant vaccine platform. This research work explores the characterisation and separation of infectious bursal disease virus-like particles (IBD-VLPs) from crude feedstock. Various characteristics were studied with high-performance size-exclusion chromatography (HP-SEC), sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE) and transmission electron microscopy (TEM) analyses. Subsequently, the separation of IBD-VLPs using polyethylene glycol (PEG)/sodium citrate-based aqueous two-phase systems (ATPSs) was conducted and optimised. Moreover, a scale-up study of the best ATPS constituted of 15% PEG 6000, 11% sodium citrate and 10% crude feedstock was performed to compare the separation performance of IBD-VLPs with and without centrifugation-assisted. The results indicated that the optimised ATPS with centrifugation-assisted for both 5 g and 50 g systems showed good recovery of IBD-VLPs of >97% in the interphase between the PEG-rich top and salt-rich bottom phases. These optimised systems also showed high removal efficiencies of impurities of >95%. The results demonstrated that aqueous two-phase extraction could be a promising technology for efficient VLPs separation.

Key words: Aqueous two-phase extraction, Infectious bursal disease virus, Polymers, Salt, Separation, Virus-like particle

Hui Yi Leong, Xiao-Qian Fu, Xiang-Yu Liu, Shan-Jing Yao, Dong-Qiang Lin. Characterisation and separation of infectious bursal disease virus-like particles using aqueous two-phase systems[J]. 中国化学工程学报, 2023, 57(5): 72-78.

参考文献

[1] E. Gómez, M.S. Lucero, M. Richetta, S. Chimeno Zoth, A. Berinstein, Infectious Bursal Disease Virus, in: J. MacDonald (Ed.), Prospects of Plant-Based Vaccines in Veterinary Medicine, Springer International Publishing, Cham, (2018) 169-187.
[2] S. Dey, D. Pathak, N. Ramamurthy, H.K. Maity, M.M. Chellappa, Infectious bursal disease virus in chickens: Prevalence, impact, and management strategies, Vet. Med. Res. Rep. 10 (2019) 85–97.
[3] C. Marusic, C. Drissi Touzani, A. Bortolami, M. Donini, C. Zanardello, C. Lico, E. Rage, S. Fellahi, M. El Houadfi, C. Terregino, S. Baschieri, The expression in plants of an engineered VP₂ protein of Infectious Bursal Disease Virus induces formation of structurally heterogeneous particles that protect from a very virulent viral strain, PLoS One 16 (2) (2021) e0247134.
[4] Y.L. Wang, N. Jiang, L.J. Fan, L. Gao, K. Li, Y.L. Gao, X.X. Niu, W.Y. Zhang, H.Y. Cui, A.J. Liu, Q. Pan, C.J. Liu, Y.P. Zhang, X.M. Wang, X.L. Qi, Development of a viral-like particle candidate vaccine against novel variant infectious bursal disease virus, Vaccines 9 (2) (2021) 142.
[5] F.S. Kibenge, A.S. Dhillon, R.G. Russell, Biochemistry and immunology of infectious bursal disease virus, J. Gen. Virol. 69 (Pt 8) (1988) 1757–1775.
[6] D.J. Jackwood, Advances in vaccine research against economically important viral diseases of food animals: Infectious bursal disease virus, Vet. Microbiol. 206 (2017) 121–125.
[7] M. Marchel, J. Niewisiewicz, A.S. Coroadinha, I.M. Marrucho, Purification of virus-like particles using aqueous biphasic systems composed of natural deep eutectic solvents, Sep. Purif. Technol. 252 (2020) 117480.
[8] H.Y. Leong, X.Q. Fu, P.L. Show, S.J. Yao, D.Q. Lin, Downstream processing of virus-like particles with aqueous two-phase systems: Applications and challenges, J. Sep. Sci. 45 (12) (2022) 2064–2076.
[9] F.F. Magalhães, A.P.M. Tavares, M.G. Freire, Advances in aqueous biphasic systems for biotechnology applications, Curr. Opin. Green Sustain. Chem. 27 (2021) 100417.
[10] K.S. Khoo, H.Y. Leong, K.W. Chew, J.W. Lim, T.C. Ling, P.L. Show, H.W. Yen, Liquid biphasic system: A recent bioseparation technology, Processes 8 (2) (2020) 149.
[11] H.Y. Leong, C.K. Chang, J.W. Lim, P.L. Show, D.Q. Lin, J.S. Chang, Liquid biphasic systems for oil-rich algae bioproducts processing, Sustainability 11 (17) (2019) 4682.
[12] Q. Wu, D.Q. Lin, Q.L. Zhang, D. Gao, S.J. Yao, Evaluation of a PEG/hydroxypropyl starch aqueous two-phase system for the separation of monoclonal antibodies from cell culture supernatant, J. Sep. Sci. 37 (4) (2014) 447–453.
[13] H.Y. Leong, C.W. Ooi, C.L. Law, A.L. Julkifle, P.L. Show, Betacyanins extraction from Hylocereus polyrhizus using alcohol/salt-based liquid biphasic partitioning system and antioxidant activity evaluation, Sep. Sci. Technol. 54 (5) (2019) 747–758.
[14] P. Du, P. Sun, S.Q. Sun, J.J. Dong, H. Dong, R.H. Liu, H.C. Guo, K.B. Mu, Z.X. Liu, Separation and purification of foot-and-mouth disease virus by multiple-stage aqueous two-phase extraction system, Process. Biochem. 77 (2019) 143–150.
[15] Y.L. Yang, H. Li, Z.J. Li, Y. Zhang, S.P. Zhang, Y. Chen, M.R. Yu, G.H. Ma, Z.G. Su, Size-exclusion HPLC provides a simple, rapid, and versatile alternative method for quality control of vaccines by characterizing the assembly of antigens, Vaccine 33 (9) (2015) 1143–1150.
[16] O. Taghavian, H. Spiegel, R. Hauck, H.M. Hafez, R. Fischer, S. Schillberg, Protective Oral Vaccination against Infectious bursal disease virus Using the Major Viral Antigenic Protein VP₂ Produced in Pichia pastoris, PLoS One 8 (12) (2013) e83210.
[17] M.S. Lee, M.Y. Wang, Y.J. Tai, S.Y. Lai, Characterization of particles formed by the precursor protein VPX of infectious bursal disease virus in insect Hi-5 cells: Implication on its proteolytic processing, J. Virol. Methods 121 (2) (2004) 191–199.
[18] D.W. Jiang, Y.C. Liu, A.P. Wang, G.P. Zhang, G.Y. Yang, Y.M. Chen, P.C. Ji, C. Liu, Y.P. Song, Y.F. Su, G.Q. Wang, J.C. Wang, B.L. Zhao, R.G. Deng, High level soluble expression and one-step purification of IBDV VP₂ protein in Escherichia coli, Biotechnol. Lett. 38 (6) (2016) 901–908.
[19] B.A. Andrews, J.A. Asenjo, Theoretical and experimental evaluation of hydrophobicity of proteins to predict their partitioning behavior in aqueous two phase systems: A review, Sep. Sci. Technol. 45 (15) (2010) 2165–2170.
[20] P.U. Joshi, D.G. Turpeinen, M. Weiss, G. Escalante-Corbin, M. Schroeder, C.L. Heldt, Tie line framework to optimize non-enveloped virus recovery in aqueous two-phase systems, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 1126-1127 (2019) 121744.
[21] Y. Guan, T.H. Lilley, T.E. Treffry, A new excluded volume theory and its application to the coexistence curves of aqueous polymer two-phase systems, Macromolecules 26 (15) (1993) 3971–3979.
[22] H.Y. Leong, C.W. Ooi, C.L. Law, A.L. Julkifle, T.C. Ling, P.L. Show, Application of liquid biphasic flotation for betacyanins extraction from peel and flesh of Hylocereus polyrhizus and antioxidant activity evaluation, Sep. Purif. Technol. 201 (2018) 156–166.
[23] H.Y. Leong, Y.K. Chang, C.W. Ooi, C.L. Law, A.L. Julkifle, P.L. Show, Liquid biphasic electric partitioning system as a novel integration process for betacyanins extraction from red-purple pitaya and antioxidant properties assessment, Front. Chem. 7 (2019) 201.

Characterisation and separation of infectious bursal disease virus-like particles using aqueous two-phase systems

Characterisation and separation of infectious bursal disease virus-like particles using aqueous two-phase systems

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