Acetic acid- and furfural-based adaptive evolution of Saccharomyces cerevisiae strains for improving stress tolerance and lignocellulosic ethanol production

doi:10.1016/j.cjche.2024.04.002

中国化学工程学报 ›› 2024, Vol. 72 ›› Issue (8): 26-33.DOI: 10.1016/j.cjche.2024.04.002

Acetic acid- and furfural-based adaptive evolution of Saccharomyces cerevisiae strains for improving stress tolerance and lignocellulosic ethanol production

Omama Rehman¹, Youduo Wu^1,2, Quan Zhang³, Jin Guo³, Cuihuan Sun⁴, Huipeng Gao³, Yaqing Xu², Rui Xu⁵, Ayesha Shahid⁶, Chuang Xue^1,2

1 State Key Laboratory of Fine Chemicals, Frontiers Science Centre for Smart Materials Oriented Chemical Engineering, Engineering Research Center of Application and Transformation for Synthetic Biology, School of Bioengineering, Dalian University of Technology, Dalian 116024, China;
2 Ningbo Institute of Dalian University of Technology, Ningbo 315016, China;
3 SINOPEC Dalian Research Institute of Petroleum and Petrochemicals Co., Ltd., Dalian 116041, China;
4 Microbial Research Institute of Liaoning Province, Chaoyang 122000, China;
5 Yunnan Provincial Rural Energy Engineering Key Laboratory, Kunming 650600, China;
6US-Pak Center for Advanced Studies in Agriculture and Food Security, University of Agriculture, Faisalabad, Pakistan

收稿日期:2023-12-10 修回日期:2024-02-19 出版日期:2024-08-28 发布日期:2024-10-17
通讯作者: Youduo Wu,E-mail:wuyouduo@dlut.edu.cn;Chuang Xue,E-mail:xue.1@dlut.edu.cn
基金资助:
This work was supported by the National Key Research and Development Program of China (2021YFC2101303), the National Natural Science Foundation of China (U22A20424 and 22378048), the Major Scientific and Technological Projects of Sinopec, the Dalian Technology Talents Project for Distinguished Young Scholars (2021RJ03), the Yunnan Provincial Rural Energy Engineering Key Laboratory (2022KF003), the National Natural Science Foundation of Liaoning Province (2023-MS-110), the Liaoning Revitalization Talents Program (XLYC2202049), the Fundamental Research Funds for the Central Universities (DUT22LK22), and the CAS Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion (E229kf0401).

Acetic acid- and furfural-based adaptive evolution of Saccharomyces cerevisiae strains for improving stress tolerance and lignocellulosic ethanol production

Omama Rehman¹, Youduo Wu^1,2, Quan Zhang³, Jin Guo³, Cuihuan Sun⁴, Huipeng Gao³, Yaqing Xu², Rui Xu⁵, Ayesha Shahid⁶, Chuang Xue^1,2

1 State Key Laboratory of Fine Chemicals, Frontiers Science Centre for Smart Materials Oriented Chemical Engineering, Engineering Research Center of Application and Transformation for Synthetic Biology, School of Bioengineering, Dalian University of Technology, Dalian 116024, China;
2 Ningbo Institute of Dalian University of Technology, Ningbo 315016, China;
3 SINOPEC Dalian Research Institute of Petroleum and Petrochemicals Co., Ltd., Dalian 116041, China;
4 Microbial Research Institute of Liaoning Province, Chaoyang 122000, China;
5 Yunnan Provincial Rural Energy Engineering Key Laboratory, Kunming 650600, China;
6US-Pak Center for Advanced Studies in Agriculture and Food Security, University of Agriculture, Faisalabad, Pakistan

Received:2023-12-10 Revised:2024-02-19 Online:2024-08-28 Published:2024-10-17
Contact: Youduo Wu,E-mail:wuyouduo@dlut.edu.cn;Chuang Xue,E-mail:xue.1@dlut.edu.cn
Supported by:
This work was supported by the National Key Research and Development Program of China (2021YFC2101303), the National Natural Science Foundation of China (U22A20424 and 22378048), the Major Scientific and Technological Projects of Sinopec, the Dalian Technology Talents Project for Distinguished Young Scholars (2021RJ03), the Yunnan Provincial Rural Energy Engineering Key Laboratory (2022KF003), the National Natural Science Foundation of Liaoning Province (2023-MS-110), the Liaoning Revitalization Talents Program (XLYC2202049), the Fundamental Research Funds for the Central Universities (DUT22LK22), and the CAS Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion (E229kf0401).

摘要/Abstract

摘要： Acetic acid and furfural are known as prevalent inhibitors deriving from pretreatment during lignocellulosic ethanol production. They negatively impact cell growth, glucose uptake and ethanol biosynthesis of Saccharomyces cerevisiae strains. Development of industrial S. cerevisiae strains with high tolerance towards these inhibitors is thus critical for efficient lignocellulosic ethanol production. In this study, the acetic acid or furfural tolerance of different S. cerevisiae strains could be significantly enhanced after adaptive evolution via serial cultivation for 40 generations under stress conditions. The acetic acid-based adaptive strain SPSC01-TA9 produced 30.5 g·L^-1 ethanol with a yield of 0.46 g·g^-1 in the presence of 9 g·L^-1 acetic acid, while the acetic acid/furfural-based adaptive strain SPSC01-TAF94 produced more ethanol of 36.2 g·L^-1 with increased yield up to 0.49 g·g^-1 in the presence of both 9 g·L^-1 acetic acid and 4 g·L^-1 furfural. Significant improvements were also observed during non-detoxified corn stover hydrolysate culture by SPSC01-TAF94, which achieved ethanol production and yield of 29.1 g·L^-1 and 0.49 g·g^-1, respectively, the growth and fermentation efficiency of acetic acid/furfural-based adaptive strain in hydrolysate was 95% higher than those of wildtype strains, indicating the acetic acid- and furfural-based adaptive evolution strategy could be an effective approach for improving lignocellulosic ethanol production. The adapted strains developed in this study with enhanced tolerance against acetic acid and furfural could be potentially contribute to economically feasible and sustainable lignocellulosic biorefinery.

关键词: Saccharomyces cerevisiae, Lignocellulosic ethanol production, Adaptive evolution, Acetic acid, Furfural

Abstract: Acetic acid and furfural are known as prevalent inhibitors deriving from pretreatment during lignocellulosic ethanol production. They negatively impact cell growth, glucose uptake and ethanol biosynthesis of Saccharomyces cerevisiae strains. Development of industrial S. cerevisiae strains with high tolerance towards these inhibitors is thus critical for efficient lignocellulosic ethanol production. In this study, the acetic acid or furfural tolerance of different S. cerevisiae strains could be significantly enhanced after adaptive evolution via serial cultivation for 40 generations under stress conditions. The acetic acid-based adaptive strain SPSC01-TA9 produced 30.5 g·L^-1 ethanol with a yield of 0.46 g·g^-1 in the presence of 9 g·L^-1 acetic acid, while the acetic acid/furfural-based adaptive strain SPSC01-TAF94 produced more ethanol of 36.2 g·L^-1 with increased yield up to 0.49 g·g^-1 in the presence of both 9 g·L^-1 acetic acid and 4 g·L^-1 furfural. Significant improvements were also observed during non-detoxified corn stover hydrolysate culture by SPSC01-TAF94, which achieved ethanol production and yield of 29.1 g·L^-1 and 0.49 g·g^-1, respectively, the growth and fermentation efficiency of acetic acid/furfural-based adaptive strain in hydrolysate was 95% higher than those of wildtype strains, indicating the acetic acid- and furfural-based adaptive evolution strategy could be an effective approach for improving lignocellulosic ethanol production. The adapted strains developed in this study with enhanced tolerance against acetic acid and furfural could be potentially contribute to economically feasible and sustainable lignocellulosic biorefinery.

Key words: Saccharomyces cerevisiae, Lignocellulosic ethanol production, Adaptive evolution, Acetic acid, Furfural

Omama Rehman, Youduo Wu, Quan Zhang, Jin Guo, Cuihuan Sun, Huipeng Gao, Yaqing Xu, Rui Xu, Ayesha Shahid, Chuang Xue. Acetic acid- and furfural-based adaptive evolution of Saccharomyces cerevisiae strains for improving stress tolerance and lignocellulosic ethanol production[J]. 中国化学工程学报, 2024, 72(8): 26-33.

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Acetic acid- and furfural-based adaptive evolution of Saccharomyces cerevisiae strains for improving stress tolerance and lignocellulosic ethanol production

Acetic acid- and furfural-based adaptive evolution of Saccharomyces cerevisiae strains for improving stress tolerance and lignocellulosic ethanol production

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