Screening and pilot-scale evaluation of a highly efficient pesticide-degrading Pseudomonas sp. strain BL5

doi:10.1016/j.cjche.2024.07.006

中国化学工程学报 ›› 2024, Vol. 74 ›› Issue (10): 287-294.DOI: 10.1016/j.cjche.2024.07.006

• Full Length Article • 上一篇下一篇

Screening and pilot-scale evaluation of a highly efficient pesticide-degrading Pseudomonas sp. strain BL5

Rui Chen¹, Xinyu Zuo¹, He Bai², Ruolin Qin¹, Zhiguo Chen², Yiyun Liu², Wenqing Cao², Jingpeng Song², Xiaoqiang Jia^1,3

1 Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China;
2 Tianjin Huakan Environmental Protection Technology Co., Ltd., Tianjin 300170, China;
3 Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, China

收稿日期:2024-03-19 修回日期:2024-07-13 接受日期:2024-07-15 出版日期:2024-10-28 发布日期:2024-08-08
通讯作者: Xiaoqiang Jia,Tel.:+86 18920698855.E-mail:xqjia@tju.edu.cn
基金资助:
This research was supported by the National Key Research and Development Program of China (2018YFA0902100), the National Natural Science Foundation of China (22178262) and the Tianjin Key Research and Development Program (23YFZCSN00110).

Screening and pilot-scale evaluation of a highly efficient pesticide-degrading Pseudomonas sp. strain BL5

Rui Chen¹, Xinyu Zuo¹, He Bai², Ruolin Qin¹, Zhiguo Chen², Yiyun Liu², Wenqing Cao², Jingpeng Song², Xiaoqiang Jia^1,3

1 Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China;
2 Tianjin Huakan Environmental Protection Technology Co., Ltd., Tianjin 300170, China;
3 Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, China

Received:2024-03-19 Revised:2024-07-13 Accepted:2024-07-15 Online:2024-10-28 Published:2024-08-08
Contact: Xiaoqiang Jia,Tel.:+86 18920698855.E-mail:xqjia@tju.edu.cn
Supported by:
This research was supported by the National Key Research and Development Program of China (2018YFA0902100), the National Natural Science Foundation of China (22178262) and the Tianjin Key Research and Development Program (23YFZCSN00110).

摘要/Abstract

摘要： The widespread use of pesticides has caused serious harm to ecosystems, necessitating effective and environmentally friendly treatment methods. Bioremediation stands out as a promising approach for pollutant treatment, wherein the metabolic activities of microorganisms can transform toxic pesticides into compounds with lower or no toxicity. In this study, we obtained eight pesticide-degrading strains from pesticide-contaminated sites through continuous enrichment and screening. Four highly efficient pesticide-degrading strains (degradation ratios exceeding 80%) were identified. Among them, Pseudomonas sp. BL5 exhibited the strongest growth (exceeding 10⁹ CFU·ml^-1) and outstanding degradation of benzene derivatives and chlorinated hydrocarbons at both laboratory and pilot scales, with degradation ratios exceeding 98% and 99.6%, respectively. This research provides new tools and insights for the bioremediation of pesticide-related pollutants.

关键词: Pesticides, Pilot-scale, Degradation, Remediation, Pseudomonas sp., Benzene

Abstract: The widespread use of pesticides has caused serious harm to ecosystems, necessitating effective and environmentally friendly treatment methods. Bioremediation stands out as a promising approach for pollutant treatment, wherein the metabolic activities of microorganisms can transform toxic pesticides into compounds with lower or no toxicity. In this study, we obtained eight pesticide-degrading strains from pesticide-contaminated sites through continuous enrichment and screening. Four highly efficient pesticide-degrading strains (degradation ratios exceeding 80%) were identified. Among them, Pseudomonas sp. BL5 exhibited the strongest growth (exceeding 10⁹ CFU·ml^-1) and outstanding degradation of benzene derivatives and chlorinated hydrocarbons at both laboratory and pilot scales, with degradation ratios exceeding 98% and 99.6%, respectively. This research provides new tools and insights for the bioremediation of pesticide-related pollutants.

Key words: Pesticides, Pilot-scale, Degradation, Remediation, Pseudomonas sp., Benzene

Rui Chen, Xinyu Zuo, He Bai, Ruolin Qin, Zhiguo Chen, Yiyun Liu, Wenqing Cao, Jingpeng Song, Xiaoqiang Jia. Screening and pilot-scale evaluation of a highly efficient pesticide-degrading Pseudomonas sp. strain BL5[J]. 中国化学工程学报, 2024, 74(10): 287-294.

参考文献

[1] V.I. Lushchak, T.M. Matviishyn, V.V. Husak, J.M. Storey, K.B. Storey, Pesticide toxicity: a mechanistic approach, EXCLI J. 17 (2018) 1101-1136.
[2] S. Chapalamadugu, G.R. Chaudhry, Hydrolysis of carbaryl by a Pseudomonas sp. and construction of a microbial consortium that completely metabolizes carbaryl, Appl. Environ. Microbiol. 57 (3) (1991) 744-750.
[3] S.A. Hasan, M.I.M. Ferreira, M.J. Koetsier, M.I. Arif, D.B. Janssen, Complete biodegradation of 4-fluorocinnamic acid by a consortium comprising Arthrobacter sp. strain G1 and Ralstonia sp. strain H1, Appl. Environ. Microbiol. 77 (2) (2011) 572-579.
[4] C. Bolognesi, A. Creus, P. Ostrosky-Wegman, R. Marcos, Micronuclei and pesticide exposure, Mutagenesis 26 (1) (2011) 19-26.
[5] R. Chen, Z. Zhao, T. Xu, X. Jia, Microbial consortium HJ-SH with very high degradation efficiency of phenanthrene, Microorganisms 11 (10) (2023) 2383.
[6] S. Kuppusamy, P. Thavamani, K. Venkateswarlu, Y.B. Lee, R. Naidu, M. Megharaj, Remediation approaches for polycyclic aromatic hydrocarbons (PAHs) contaminated soils: technological constraints, emerging trends and future directions, Chemosphere 168 (2017) 944-968.
[7] S. Bose, P.S. Kumar, D.V N. Vo, N. Rajamohan, R. Saravanan, Microbial degradation of recalcitrant pesticides: a review, Environ. Chem. Lett. 19 (4) (2021) 3209-3228.
[8] C.C. Azubuike, C.B. Chikere, G.C. Okpokwasili, Bioremediation techniques-classification based on site of application: principles, advantages, limitations and prospects, World J. Microbiol. Biotechnol. 32 (11) (2016) 180.
[9] A. Dadrasnia, K.S. Chuan Wei, N. Shahsavari, M.S. Azirun, S. Ismail, Biosorption potential of bacillus salmalaya strain 139SI for removal of Cr(VI) from aqueous solution, Int. J. Environ. Res. Public Health 12 (12) (2015) 15321-15338.
[10] S.D. Copley, Evolution of efficient pathways for degradation of anthropogenic chemicals, Nat. Chem. Biol. 5 (8) (2009) 559-566.
[11] R. Chen, R.L. Qin, H. Bai, X.Q. Jia, Recent advances and optimization strategies for the microbial degradation of PCBs: from monocultures to microbial consortia, Crit. Rev. Environ. Sci. Technol. 54 (14) (2024) 1023-1049.
[12] A. Aswathi, A. Pandey, R.K. Sukumaran, Rapid degradation of the organophosphate pesticide-Chlorpyrifos by a novel strain of Pseudomonas nitroreducens AR-3, Bioresour. Technol. 292 (2019) 122025.
[13] D.M. Dash, W.J. Osborne, Rapid biodegradation and biofilm-mediated bioremoval of organophosphorus pesticides using an indigenous Kosakonia oryzae strain-VITPSCQ3 in a Vertical-flow Packed Bed Biofilm Bioreactor, Ecotoxicol. Environ. Saf. 192 (2020) 110290.
[14] B. Xu, Q.J. Sun, J.C. Lan, W.M. Chen, C.C. Hsueh, B.Y. Chen, Exploring the glyphosate-degrading characteristics of a newly isolated, highly adapted indigenous bacterial strain, Providencia rettgeri GDB 1, J Biosci Bioeng 128 (1) (2019) 80-87.
[15] L. Zhang, C. Zhang, Z. Cheng, Y. Yao, J. Chen, Biodegradation of benzene, toluene, ethylbenzene, and o-xylene by the bacterium mycobacterium cosmeticum byf-4, Chemosphere 90 (4) (2013) 1340-1347.
[16] S. Schulze, A. Tiehm, Assessment of microbial natural attenuation in groundwater polluted with gasworks residues, Water Sci. Technol. 50 (5) (2004) 347-353.
[17] C.M. Kao, J. Prosser, Evaluation of natural attenuation rate at a gasoline spill site, J. Med. Internet Res. 82 (3) (2001) 275-289.
[18] D. de Lima-Morales, D. Chaves-Moreno, M.L. Wos-Oxley, R. Jauregui, R. Vilchez-Vargas, D.H. Pieper, Degradation of benzene by pseudomonas veronii 1YdBTEX2 and 1YB2 is catalyzed by enzymes encoded in distinct catabolism gene clusters, Appl. Environ. Microbiol. 82 (1) (2016) 167-173.
[19] H.B. Yu, B.J. Kim, B.E. Rittmann, The roles of intermediates in biodegradation of benzene, toluene, and p-xylene by Pseudomonas putida F1, Biodegradation 12 (6) (2001) 455-463.
[20] B. Mahendran, N.C. Choi, J.W. Choi, D.J. Kim, Effect of dissolved oxygen regime on growth dynamics of Pseudomonas spp during benzene degradation, Appl. Microbiol. Biotechnol. 71 (3) (2006) 350-354.
[21] R. Dolphen, C. Treesubsuntorn, N. Santawee, A. Setsungnoen, P. Thiravetyan, Modified coir pith with glucose syrup as a supporter in non-external nutrient supplied biofilter for benzene removal by Bacillus megaterium, Environ. Technol. 41 (27) (2020) 3607-3618.
[22] H. Mohammadpour, M. Shahriarinour, R. Yousefi, Benzene degradation by free and immobilized Bacillus glycinifermantans strain GO-13T using GO sheets, Pol. J. Environ. Stud. 29 (4) (2020) 2783-2793.
[23] J. Dou, A. Ding, X. Liu, Y. Du, D. Deng, J. Wang, Anaerobic benzene biodegradation by a pure bacterial culture of Bacillus cereus under nitrate reducing conditions, J. Environ. Sci. China 22 (5) (2010) 709-715.
[24] A. Hernandez-Santana, J. Dussan, Lysinibacillus sphaericus proved to have potential for the remediation of petroleum hydrocarbons, Soil Sediment Contam. 27 (6) (2018) 538-549.
[25] A. Wongbunmak, S. Khiawjan, M. Suphantharika, T. Pongtharangkul, BTEX- and naphthalene-degrading bacterium Microbacterium esteraromaticum strain SBS1-7 isolated from estuarine sediment, J. Hazard. Mater. 339 (2017) 82-90.
[26] X. Liang, C.E. Devine, J. Nelson, B. Sherwood Lollar, S. Zinder, E.A. Edwards, Anaerobic conversion of chlorobenzene and benzene to CH₄ and CO₂ in bioaugmented microcosms, Environ. Sci. Technol. 47 (5) (2013) 2378-2385.
[27] D. Mikailitchenko, A. Marbeuf, H.A.J. Oonk, Destabilizing effects of chlorine on complexes belonging to the benzene family: p-C₆H₄Cl₂-C₆F₆ and p-C₆H₄CH₃Cl-C₆F₆ systems, Chem. Mater., 11 (1999) 2866-2871.
[28] R. Margesin, G. Walder, F. Schinner, Bioremediation assessment of a BTEX-contaminated soil, Acta Biotechnol. 23 (1) (2003) 29-36.
[29] D.Q. Zhang, P.J. He, L.M. Shao, Effect of leachate inoculum on biopretreatment of municipal solid waste by a combined hydrolytic-aerobic process, J. Environ. Sci. 21 (8) (2009) 1162-1168.
[30] R. Zhang, R. Cao, C. Ye, C. Li, Biodegradation of organic contaminants in sediment of littoral zone by facultative anaerobic bacteria, Environ Pollution & Control, 34 (2012) 61-65,69.
[31] N.R. Mullins, A.J. Daugulis, The biological treatment of synthetic fracking fluid in an extractive membrane bioreactor: selective transport and biodegradation of hydrophobic and hydrophilic contaminants, J. Hazard. Mater. 371 (2019) 734-742.
[32] L.A. Pratt, R. Kolter, Genetic analyses of bacterial biofilm formation, Curr. Opin. Microbiol. 2 (6) (1999) 598-603.
[33] P.L. Stelmack, M.R. Gray, M.A. Pickard, Bacterial adhesion to soil contaminants in the presence of surfactants, RSC Adv. 65 (1) (1999) 163-168.
[34] A.M.J. Law, M.D. Aitken, Bacterial chemotaxis to naphthalene desorbing from a nonaqueous liquid, Appl. Environ. Microbiol. 69 (10) (2003) 5968-5973.
[35] M.T. Rose, F. Sanchez-Bayo, A.N. Crossan, I.R. Kennedy, Pesticide removal from cotton farm tailwater by a pilot-scale ponded wetland, Chemosphere 63 (11) (2006) 1849-1858.
[36] C.N. Albers, L. Feld, L. Ellegaard-Jensen, J. Aamand, Degradation of trace concentrations of the persistent groundwater pollutant 2, 6-dichlorobenzamide (BAM) in bioaugmented rapid sand filters, Water Res. 83 (2015) 61-70.
[37] M.D. Schostag, A. Gobbi, M.N. Fini, L. Ellegaard-Jensen, J. Aamand, L.H. Hansen, J. Muff, C.N. Albers, Combining reverse osmosis and microbial degradation for remediation of drinking water contaminated with recalcitrant pesticide residue, Water Res. 216 (2022) 118352.
[38] M. Kumar, L. Philip, Biodegradation of endosulfan-contaminated soil in a pilot-scale reactor-bioaugmented with mixed bacterial culture, J. Environ. Sci. Health Part B 42 (6) (2007) 707-715.
[39] P.A. Karas, S. Makri, E.S. Papadopoulou, C. Ehaliotis, U. Menkissoglu-Spiroudi, D.G. Karpouzas, The potential of organic substrates based on mushroom substrate and straw to dissipate fungicides contained in effluents from the fruit-packaging industry - Is there a role for Pleurotus ostreatus? Ecotoxicol. Environ. Saf. 124 (2016) 447-454.
[40] P.A. Karas, C. Perruchon, E. Karanasios, E.S. Papadopoulou, E. Manthou, S. Sitra, C. Ehaliotis, D.G. Karpouzas, Integrated biodepuration of pesticide-contaminated wastewaters from the fruit-packaging industry using biobeds: Bioaugmentation, risk assessment and optimized management, J. Hazard. Mater. 320 (2016) 635-644.

Screening and pilot-scale evaluation of a highly efficient pesticide-degrading Pseudomonas sp. strain BL5

Screening and pilot-scale evaluation of a highly efficient pesticide-degrading Pseudomonas sp. strain BL5

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	Wenwen Gao, Yuhuan Wang, Wang Li, Zhifang Zhang, Ting Su, Miao Mu, Ying Gong, Rui Dang, Rui Bai, E Zheng, Wei Zhao. A Z-scheme LaFeO₃-CuFe₂O₄ composite for sulfate radical-based photocatalytic process: Synergistic effect and mechanism[J]. 中国化学工程学报, 2024, 73(9): 256-269.
[2]	Aishun Ma, Hanlin Qian, Hongxia Liu, Sili Ren. Preparation of K_ω-g-C₃N₄ composite loaded on magnetic attapulgite and its degradation performance for malachite green[J]. 中国化学工程学报, 2024, 71(7): 110-121.
[3]	Pengfei Wu, Zhaolong Liu, Li Wu, Yingkun Zhang, Bing Wang, Zhanghao Cheng, Wenquan Cui, Xiangyang Lv, Qingling Liu. Mesoporous amorphous FeOOH-encapsulated BiO_2–x photocatalyst with harnessing broad spectrum toward activation of persulfate for tetracycline degradation[J]. 中国化学工程学报, 2024, 71(7): 235-248.
[4]	Shuyi Shen, Song Guo, Sining Chen, Jinhua Zhao. Study on thermal decomposition kinetics of azobenzene-4,4′-dicarboxylic acid by using compensation parameter method and nonlinear fitting evaluation[J]. 中国化学工程学报, 2024, 70(6): 269-279.
[5]	Yuhang Wei, Qingpeng Zhu, Weiwei Xie, Xinyue Wang, Song Li, Zhiming Chen. Biocatalytic enhancement of laccase immobilized on ZnFe₂O₄ nanoparticles and its application for degradation of textile dyes[J]. 中国化学工程学报, 2024, 68(4): 216-223.
[6]	Hongnan Chen, Yifei Sun, Bojian Cao, Minglong Wang, Ming Wang, Jinrong Zhong, Changyu Sun, Guangjin Chen. Enhanced gas production and CO₂ storage in hydrate-bearing sediments via pre-depressurization and rapid CO₂ injection[J]. 中国化学工程学报, 2024, 67(3): 126-134.
[7]	Kun Li, Han Tang, Shuangshuang Li, Zixuan Huang, Bei Liu, Chun Deng, Changyu Sun, Guangjin Chen. Highly efficient CO₂ capture using 2-methylimidazole aqueous solution on laboratory and pilot-scale[J]. 中国化学工程学报, 2024, 67(3): 148-156.
[8]	Siyue Wang, Jinhong Li, Qingxin Xu, Shengjie Song, Yu'ni Jiang, Lidong Chen, Xin Shi, Weiguo Cheng. Synthesis of spherical nano-ZSM-5 zeolite with intergranular mesoporous for alkylation of ethylbenzene with ethanol to produce m-diethylbenzene[J]. 中国化学工程学报, 2024, 67(3): 298-309.
[9]	Jiangtao Cai, Qingfu Huang, Huan Chen, Tao Zhang, Bo Niu, Yayun Zhang, Donghui Long. Evaluating two stages of silicone-containing arylene resin oxidation via experiment and molecular simulation[J]. 中国化学工程学报, 2024, 66(2): 189-202.
[10]	Hao Yuan, Xinhai Sun, Shuai Zhang, Weilong Shi, Feng Guo. Achieving high-efficient photocatalytic persulfate-activated degradation of tetracycline via carbon dots modified MIL-101(Fe) octahedrons[J]. 中国化学工程学报, 2024, 66(2): 298-309.
[11]	Hongtao Wang, Xiangrui Fan, Mingming Yan, Tianyu Guo, Xingfa Li, Chao Chen, Yu Qi. BiOBr nanosheets coupling with biomass carbon derived from locust leaves for enhanced photocatalytic degradation of rhodamine B[J]. 中国化学工程学报, 2024, 74(10): 31-43.
[12]	Donghui Li, Wenzhe Wu, Xue Ren, Xixi Zhao, Hongbing Song, Meng Xiao, Quanhong Zhu, Hengjun Gai, Tingting Huang. Enhanced activation of peroxymonosulfate by Fe/N co-doped ordered mesoporous carbon with dual active sites for efficient removal of m-cresol[J]. 中国化学工程学报, 2024, 65(1): 130-144.
[13]	Jinzhi Cui, Guiqiao Wang, Xing Rong, Wensu Gao, Yaxin Lu, Yawen Luo, Lichao Zhang, Zhongfa Cheng, Canzhu Gao. Removal of kathon by UV-C activated hydrogen peroxide: Kinetics, mechanisms, and enhanced biodegradability assessment[J]. 中国化学工程学报, 2024, 65(1): 178-187.
[14]	Chun Bai, Huifang Zhang, Qinglong Luo, Xiushen Ye, Haining Liu, Quan Li, Jun Li, Zhijian Wu. Boron separation by adsorption and flotation with Mg–Al-LDHs and SDBS from aqueous solution[J]. 中国化学工程学报, 2023, 61(9): 192-200.
[15]	Ali Nikkhah, Hasan Nikkhah, Hadis langari, Alireza Nouri, Abdul Wahab Mohammad, Ang Wei Lun, Ng law Yong, Rosiah Rohani, Ebrahim Mahmoudi. MXene: From synthesis to environment remediation[J]. 中国化学工程学报, 2023, 61(9): 260-280.