SCI和EI收录∣中国化工学会会刊

›› 2009, Vol. 17 ›› Issue (2): 350-354.

• • 上一篇    下一篇

Efficient and Comprehensive Utilization of Hemicellulose in the Corn Stover

高鹏飞1,2,3, 范代娣1,2,3, 骆艳娥1,2,3, 马晓轩1,2,3, 马沛1,2,3, 惠俊峰1,2,3, 朱晨辉1,2   

  1. 1. Shaanxi Key Laboratory of Degradable Biomedical Materials, Northwest University, Xi'an 710069, China;
    2. Shaanxi R&D Center of Biomaterial and Fermentation Engineering, Northwest University, Xi'an 710069, China;
    3. College of Chemical Engineering, Northwest University, Xi'an 710069, China
  • 收稿日期:2008-11-28 修回日期:2009-02-25 出版日期:2009-04-28 发布日期:2009-04-28
  • 通讯作者: FAN Daidi,E-mail:fandaidi@nwu.edu.cn
  • 基金资助:
    Supported by the Agriculture Application Investigation and Improvement Item of New Countryside Construction and Promotion Project of the Bureau of Science and Technology in Xi'an (NC08005)

Efficient and Comprehensive Utilization of Hemicellulose in the Corn Stover

GAO Pengfei1,2,3, FAN Daidi1,2,3, LUO Yan'e1,2,3, MA Xiaoxuan1,2,3, MA Pei1,2,3, HUI Junfeng1,2,3, ZHU Chenhui1,2   

  1. 1. Shaanxi Key Laboratory of Degradable Biomedical Materials, Northwest University, Xi'an 710069, China;
    2. Shaanxi R&D Center of Biomaterial and Fermentation Engineering, Northwest University, Xi'an 710069, China;
    3. College of Chemical Engineering, Northwest University, Xi'an 710069, China
  • Received:2008-11-28 Revised:2009-02-25 Online:2009-04-28 Published:2009-04-28
  • Supported by:
    Supported by the Agriculture Application Investigation and Improvement Item of New Countryside Construction and Promotion Project of the Bureau of Science and Technology in Xi'an (NC08005)

摘要: Pretreatment of the corn stover powder by dilute sulphuric acid(solid-liquid ratio 1:20) at 130℃ for 30 min was carried out with 89.09% of the hemicellulose removed.After filtration,the xylose-rich corn stover pretreatment liquid,whose fermentable sugar was from hemicellulose hydrolysis only,consisting of 81.16% xylose and 15.27% glucose,was used to cultivate genetic recombinant Escherichia coli BL21 with human-like collagen(HLC) expression enhanced by 50.00% and 63.71% xylose consumption.

关键词: corn stover, Escherichia coli BL21, human-like collagen, combined sugar

Abstract: Pretreatment of the corn stover powder by dilute sulphuric acid(solid-liquid ratio 1:20) at 130℃ for 30 min was carried out with 89.09% of the hemicellulose removed.After filtration,the xylose-rich corn stover pretreatment liquid,whose fermentable sugar was from hemicellulose hydrolysis only,consisting of 81.16% xylose and 15.27% glucose,was used to cultivate genetic recombinant Escherichia coli BL21 with human-like collagen(HLC) expression enhanced by 50.00% and 63.71% xylose consumption.

Key words: corn stover, Escherichia coli BL21, human-like collagen, combined sugar