SCI和EI收录∣中国化工学会会刊

Chinese Journal of Chemical Engineering ›› 2013, Vol. 21 ›› Issue (6): 670-675.DOI: 10.1016/S1004-9541(13)60528-5

• 生物技术与生物工程 • 上一篇    下一篇

Immobilization of Papain in Biosilica Matrix and Its Catalytic Property

周丽亚1, 王翠1, 姜艳军1,2, 高静1   

  1. 1 School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130, China;
    2 National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China
  • 收稿日期:2012-06-25 修回日期:2012-10-20 出版日期:2013-06-28 发布日期:2013-07-03
  • 通讯作者: GAO Jing
  • 基金资助:

    Supported by the National Natural Science Foundation of China (21006020, 21276060, 21276062), the Natural Science Foundation of Hebei Province (B2010000035, B2011202095), the Science and Technology Research Key Project of Higher School in Hebei Province (ZD2010118)

Immobilization of Papain in Biosilica Matrix and Its Catalytic Property

ZHOU Liya1, WANG Cui1, JIANG Yanjun1,2, GAO Jing1   

  1. 1 School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130, China;
    2 National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China
  • Received:2012-06-25 Revised:2012-10-20 Online:2013-06-28 Published:2013-07-03
  • Contact: GAO Jing
  • Supported by:

    Supported by the National Natural Science Foundation of China (21006020, 21276060, 21276062), the Natural Science Foundation of Hebei Province (B2010000035, B2011202095), the Science and Technology Research Key Project of Higher School in Hebei Province (ZD2010118)

摘要: A novel method was developed for papain immobilization through a biomimetic silicification process induced by papain. By incubating papain in a silica precursor solution, the papain-silica composite formed rapidly and papain was encapsulated. The encapsulation efficiency and the recovery activity were 82.60% and 83.09%, respectively. Compared with enzymes and biomolecules immobilized in biosilica matrix in the presence of additional silica-precipitating species, this papain encapsulation process, a biomimetic approach, realized high encapsulation efficiency by its autosilification activity under mild conditions (near-neutral pH and ambient temperature). Furthermore, the encapsulated papain exhibits enhanced thermal, pH, recycling and storage stabilities. Kinetic analysis showed that the biomimetic silica matrix did not significantly hinder the mass transport of substrate or the release of product.

关键词: papain, silica matrix, encapsulation, biosilicification, biomimetic

Abstract: A novel method was developed for papain immobilization through a biomimetic silicification process induced by papain. By incubating papain in a silica precursor solution, the papain-silica composite formed rapidly and papain was encapsulated. The encapsulation efficiency and the recovery activity were 82.60% and 83.09%, respectively. Compared with enzymes and biomolecules immobilized in biosilica matrix in the presence of additional silica-precipitating species, this papain encapsulation process, a biomimetic approach, realized high encapsulation efficiency by its autosilification activity under mild conditions (near-neutral pH and ambient temperature). Furthermore, the encapsulated papain exhibits enhanced thermal, pH, recycling and storage stabilities. Kinetic analysis showed that the biomimetic silica matrix did not significantly hinder the mass transport of substrate or the release of product.

Key words: papain, silica matrix, encapsulation, biosilicification, biomimetic