Lipid characterization of an arachidonic acid-rich oil producing fungus Mortierella alpina

doi:10.1016/j.cjche.2015.04.015

Chinese Journal of Chemical Engineering ›› 2015, Vol. 23 ›› Issue (7): 1183-1187.DOI: 10.1016/j.cjche.2015.04.015

Lipid characterization of an arachidonic acid-rich oil producing fungus Mortierella alpina

Wenjia Wu¹, Jiacheng Yan^1,2, Xiaojun Ji¹, Xin Zhang³, Jingsheng Shang¹, Lina Sun¹, Lujing Ren¹, He Huang¹

1 State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China;
2 Faculty of Science, Hong Kong Baptist University, Hong Kong 999077, China;
3 College of Sciences, Nanjing Tech University, Nanjing 211816, China

收稿日期:2014-02-27 修回日期:2014-09-02 出版日期:2015-07-28 发布日期:2015-08-21
通讯作者: Xiaojun Ji
基金资助:
Supported by the National Science Foundation for Distinguished Young Scholars of China (21225626), the National Basic Research Program of China (2011CBA00800), the National Natural Science Foundation of China (21376002, 21476111), the Natural Science Foundation of Jiangsu Province (BK20131405), the National High-Tech R&D Program of China (2014AA021703), and the Priority Academic Program Development of Jiangsu Higher Education Institutions.

Lipid characterization of an arachidonic acid-rich oil producing fungus Mortierella alpina

Wenjia Wu¹, Jiacheng Yan^1,2, Xiaojun Ji¹, Xin Zhang³, Jingsheng Shang¹, Lina Sun¹, Lujing Ren¹, He Huang¹

1 State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China;
2 Faculty of Science, Hong Kong Baptist University, Hong Kong 999077, China;
3 College of Sciences, Nanjing Tech University, Nanjing 211816, China

Received:2014-02-27 Revised:2014-09-02 Online:2015-07-28 Published:2015-08-21
Contact: Xiaojun Ji
Supported by:
Supported by the National Science Foundation for Distinguished Young Scholars of China (21225626), the National Basic Research Program of China (2011CBA00800), the National Natural Science Foundation of China (21376002, 21476111), the Natural Science Foundation of Jiangsu Province (BK20131405), the National High-Tech R&D Program of China (2014AA021703), and the Priority Academic Program Development of Jiangsu Higher Education Institutions.

摘要/Abstract

摘要： Mortierella alpina has been considered as the most effective producer of arachidonic acid (ARA)-rich oil. It was found that several methods could improve the percentage of ARA in total lipids successfully, as they activated the desaturation system on the endoplasmic reticulum. Additionally, in M. alpina the ARA exists in several forms, such as triacylglycerol (TAG), and diacylglycerol (DAG). These forms are caused by different acyltransferases and they determine the nutrient value of the microbial oil. However, few works revealed detailed fatty acid distribution among lipid classes, which to some extent impeded the accurate regulation in ARA accumulation. Herein, this paper gives information on the accumulation process of main lipid classes and the changes of fatty acid composition in these lipids during ARA accumulation period inM. alpina. The result demonstrates that TAG was the dominant component of the total lipids, and it is the main form for ARA storage. The ARA enrichment stage occurred during 168-192 h when the amount of total lipids maintained steady. Further analysis indicated that the newly formed ARA-TAG might come from the incorporation and modification of saturated and monounsaturated fatty acids in other lipid classes. This work could be helpful for further optimization of ARA-rich TAG production.

关键词: Arachidonic acid, Lipid characterization, Triacylglycerol, Mortierella alpina

Abstract: Mortierella alpina has been considered as the most effective producer of arachidonic acid (ARA)-rich oil. It was found that several methods could improve the percentage of ARA in total lipids successfully, as they activated the desaturation system on the endoplasmic reticulum. Additionally, in M. alpina the ARA exists in several forms, such as triacylglycerol (TAG), and diacylglycerol (DAG). These forms are caused by different acyltransferases and they determine the nutrient value of the microbial oil. However, few works revealed detailed fatty acid distribution among lipid classes, which to some extent impeded the accurate regulation in ARA accumulation. Herein, this paper gives information on the accumulation process of main lipid classes and the changes of fatty acid composition in these lipids during ARA accumulation period inM. alpina. The result demonstrates that TAG was the dominant component of the total lipids, and it is the main form for ARA storage. The ARA enrichment stage occurred during 168-192 h when the amount of total lipids maintained steady. Further analysis indicated that the newly formed ARA-TAG might come from the incorporation and modification of saturated and monounsaturated fatty acids in other lipid classes. This work could be helpful for further optimization of ARA-rich TAG production.

Key words: Arachidonic acid, Lipid characterization, Triacylglycerol, Mortierella alpina

Wenjia Wu, Jiacheng Yan, Xiaojun Ji, Xin Zhang, Jingsheng Shang, Lina Sun, Lujing Ren, He Huang. Lipid characterization of an arachidonic acid-rich oil producing fungus Mortierella alpina[J]. Chinese Journal of Chemical Engineering, 2015, 23(7): 1183-1187.

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Lipid characterization of an arachidonic acid-rich oil producing fungus Mortierella alpina

Lipid characterization of an arachidonic acid-rich oil producing fungus Mortierella alpina

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