A cold adapt and ethanol tolerant endoglucanase from a marine Bacillus subtilis

doi:10.1016/j.cjche.2018.02.007

Chinese Journal of Chemical Engineering ›› 2018, Vol. 26 ›› Issue (12): 2601-2606.DOI: 10.1016/j.cjche.2018.02.007

• Biotechnology and Bioengineering • 上一篇下一篇

A cold adapt and ethanol tolerant endoglucanase from a marine Bacillus subtilis

Dongsheng Xue¹, Xuhao Zeng¹, Chunjie Gong¹, Dongqiang Lin², Shanjing Yao²

1 Key Laboratory of Fermentation Engineering(Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology, Wuhan 430068, China;
2 Department of Chemical and Bioengineering, Zhejiang University, Hangzhou 310027, China

收稿日期:2017-11-11 修回日期:2018-01-31 出版日期:2018-12-28 发布日期:2019-01-09
通讯作者: Shanjing Yao
基金资助:
Supported by the Science and Technology Research Project of Hubei Provicial Department of Education(B2017041).

A cold adapt and ethanol tolerant endoglucanase from a marine Bacillus subtilis

Dongsheng Xue¹, Xuhao Zeng¹, Chunjie Gong¹, Dongqiang Lin², Shanjing Yao²

1 Key Laboratory of Fermentation Engineering(Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology, Wuhan 430068, China;
2 Department of Chemical and Bioengineering, Zhejiang University, Hangzhou 310027, China

Received:2017-11-11 Revised:2018-01-31 Online:2018-12-28 Published:2019-01-09
Contact: Shanjing Yao
Supported by:
Supported by the Science and Technology Research Project of Hubei Provicial Department of Education(B2017041).

摘要/Abstract

摘要： The catalytic properties and thermodynamic kinetics of the endoglucanase from a marine Bacillus subtilis were analyzed. Optimum pH and temperature of the endoglucanase activity were 5.0 and 35℃. The endoglucanase activity, melt point temperature was 1.13 folds (247.02 U·ml^-1), 2.1℃ higher (39.2℃) in 6% ethanol solution than that (218.60 U·ml^-1), (37.1℃) in free ethanol. At 40℃-55℃, Gibbs free energy, ΔG, and the content of α-helix was higher in 6% ethanol solution than that in ethanol free solution. The increasing of α-helix content led to higher activity and better thermostability in ethanol solution. The cold adapt ethanol tolerant endoglucanase was valuable for bioethanol product by simultaneous saccharification and fermentation process.

关键词: Endoglucanase, Ethanol tolerant, Cold adapt, Bioethanol

Abstract: The catalytic properties and thermodynamic kinetics of the endoglucanase from a marine Bacillus subtilis were analyzed. Optimum pH and temperature of the endoglucanase activity were 5.0 and 35℃. The endoglucanase activity, melt point temperature was 1.13 folds (247.02 U·ml^-1), 2.1℃ higher (39.2℃) in 6% ethanol solution than that (218.60 U·ml^-1), (37.1℃) in free ethanol. At 40℃-55℃, Gibbs free energy, ΔG, and the content of α-helix was higher in 6% ethanol solution than that in ethanol free solution. The increasing of α-helix content led to higher activity and better thermostability in ethanol solution. The cold adapt ethanol tolerant endoglucanase was valuable for bioethanol product by simultaneous saccharification and fermentation process.

Key words: Endoglucanase, Ethanol tolerant, Cold adapt, Bioethanol

Dongsheng Xue, Xuhao Zeng, Chunjie Gong, Dongqiang Lin, Shanjing Yao. A cold adapt and ethanol tolerant endoglucanase from a marine Bacillus subtilis[J]. Chinese Journal of Chemical Engineering, 2018, 26(12): 2601-2606.

Dongsheng Xue, Xuhao Zeng, Chunjie Gong, Dongqiang Lin, Shanjing Yao. A cold adapt and ethanol tolerant endoglucanase from a marine Bacillus subtilis[J]. Chin.J.Chem.Eng., 2018, 26(12): 2601-2606.

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A cold adapt and ethanol tolerant endoglucanase from a marine Bacillus subtilis

A cold adapt and ethanol tolerant endoglucanase from a marine Bacillus subtilis

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