Increased sedimentation of a Pseudomonas-Saccharomyces microbial consortium producing medium chain length polyhydroxyalkanoates

doi:10.1016/j.cjche.2018.11.013

中国化学工程学报 ›› 2019, Vol. 27 ›› Issue (7): 1659-1665.DOI: 10.1016/j.cjche.2018.11.013

• Biotechnology and Bioengineering • 上一篇下一篇

Increased sedimentation of a Pseudomonas-Saccharomyces microbial consortium producing medium chain length polyhydroxyalkanoates

Chang Liu¹, Lin Qi¹, Songyuan Yang¹, Yun He¹, Xiaoqiang Jia^1,2,3

1 Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China;
2 Key Laboratory of Systems Bioengineering(Tianjin University), Ministry of Education, Tianjin 300072, China;
3 SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin 300072, China

收稿日期:2018-08-03 出版日期:2019-07-28 发布日期:2019-10-14
通讯作者: Xiaoqiang Jia

Increased sedimentation of a Pseudomonas-Saccharomyces microbial consortium producing medium chain length polyhydroxyalkanoates

Chang Liu¹, Lin Qi¹, Songyuan Yang¹, Yun He¹, Xiaoqiang Jia^1,2,3

1 Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China;
2 Key Laboratory of Systems Bioengineering(Tianjin University), Ministry of Education, Tianjin 300072, China;
3 SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin 300072, China

Received:2018-08-03 Online:2019-07-28 Published:2019-10-14
Contact: Xiaoqiang Jia

摘要/Abstract

摘要： Concerns about feasibility, separability, settleability, efficiency once hampered studies on polyhydroxyalkanoates (PHAs) production, which mainly focused on single strain microorganism or activated sludge rather than artificial microbial consortia. Here, a medium chain length PHAs (mcl-PHAs) producing Pseudomonas-Saccharomyces consortium with xylose as the main substrate was studied. Mcl-PHAs accumulation increased from 12.69 mg·L^-1 to 152.3 mg·L^-1 without any optimization method. The presence of Saccharomyces cerevisiae, though in a relatively low concentration, improved the sedimentation of cell mass of the mixed culture by 60%. Reasons for better sedimentation of the consortium were complex:first, the length of Pseudomonas putida increased two to three times in the consortium; second, the positive surface charge of P. putida was neutralized by S. cerevisiae; third, the adhesion proteins on the surface of S. cerevisiae interacted with the P. putida.

关键词: Medium chain length, polyhydroxyalkanoates, Pseudomonas-Saccharomyces consortium, Sedimentation, Xylose

Abstract: Concerns about feasibility, separability, settleability, efficiency once hampered studies on polyhydroxyalkanoates (PHAs) production, which mainly focused on single strain microorganism or activated sludge rather than artificial microbial consortia. Here, a medium chain length PHAs (mcl-PHAs) producing Pseudomonas-Saccharomyces consortium with xylose as the main substrate was studied. Mcl-PHAs accumulation increased from 12.69 mg·L^-1 to 152.3 mg·L^-1 without any optimization method. The presence of Saccharomyces cerevisiae, though in a relatively low concentration, improved the sedimentation of cell mass of the mixed culture by 60%. Reasons for better sedimentation of the consortium were complex:first, the length of Pseudomonas putida increased two to three times in the consortium; second, the positive surface charge of P. putida was neutralized by S. cerevisiae; third, the adhesion proteins on the surface of S. cerevisiae interacted with the P. putida.

Key words: Medium chain length, polyhydroxyalkanoates, Pseudomonas-Saccharomyces consortium, Sedimentation, Xylose

Chang Liu, Lin Qi, Songyuan Yang, Yun He, Xiaoqiang Jia. Increased sedimentation of a Pseudomonas-Saccharomyces microbial consortium producing medium chain length polyhydroxyalkanoates[J]. 中国化学工程学报, 2019, 27(7): 1659-1665.

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Increased sedimentation of a Pseudomonas-Saccharomyces microbial consortium producing medium chain length polyhydroxyalkanoates

Increased sedimentation of a Pseudomonas-Saccharomyces microbial consortium producing medium chain length polyhydroxyalkanoates

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