SCI和EI收录∣中国化工学会会刊

中国化学工程学报 ›› 2020, Vol. 28 ›› Issue (8): 2037-2050.DOI: 10.1016/j.cjche.2020.04.003

• Reviews • 上一篇    下一篇

Multienzyme co-immobilization-based bioelectrode: Design of principles and bioelectrochemical applications

Ranran Wu1, Haiyan Song1, Yuanming Wang1,2, Lei Wang3, Zhiguang Zhu1,2   

  1. 1 Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West Seventh Avenue, Tianjin Airport Economic Area, Tianjin 300308, China;
    2 University of Chinese Academy of Sciences, 19 A Yuquan Road, Shijingshan District, Beijing 100049, China;
    3 National Human Genetic Resource Center, 12 Dahuisi Road, Haidian District, Beijing 100081, China
  • 收稿日期:2020-01-14 修回日期:2020-03-13 出版日期:2020-08-28 发布日期:2020-09-19
  • 通讯作者: Zhiguang Zhu
  • 基金资助:
    This study was supported by the National Natural Science Foundation of China (21878324, 21706273) and the CAS Pioneer Hundred Talent Program (Type C, reference # 2016-081).

Multienzyme co-immobilization-based bioelectrode: Design of principles and bioelectrochemical applications

Ranran Wu1, Haiyan Song1, Yuanming Wang1,2, Lei Wang3, Zhiguang Zhu1,2   

  1. 1 Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West Seventh Avenue, Tianjin Airport Economic Area, Tianjin 300308, China;
    2 University of Chinese Academy of Sciences, 19 A Yuquan Road, Shijingshan District, Beijing 100049, China;
    3 National Human Genetic Resource Center, 12 Dahuisi Road, Haidian District, Beijing 100081, China
  • Received:2020-01-14 Revised:2020-03-13 Online:2020-08-28 Published:2020-09-19
  • Contact: Zhiguang Zhu
  • Supported by:
    This study was supported by the National Natural Science Foundation of China (21878324, 21706273) and the CAS Pioneer Hundred Talent Program (Type C, reference # 2016-081).

摘要: Enzyme cascade reactions play significant roles in bioelectrochemical processes because they permit more complex reactions. Co-immobilization of multienzyme on the electrode could help to facilitate substrate/intermediate transfer among different enzymes and electron transfer from enzyme active sites to the electrode with high stability and retrievability. Different co-immobilization strategies to construct multienzyme bioelectrodes have been widely reported, however, up to now, they have barely been reviewed. In this review, we focus on recent state-of-the-art techniques for constructing co-immobilized multienzyme electrodes including random and positional co-immobilization. Particular attention is given to strategies such as multienzyme complex and surface display. Cofactor co-immobilization on the electrode is also crucial for the enhancement of catalytic reaction and electron transfer, yet, few studies have been reported. The up-to-date advances in bioelectrochemical applications of multienzyme bioelectrodes are also presented. Finally, key challenges and future perspectives are discussed.

关键词: Multienzyme co-immobilization, Bioelectrode, Bioelectrochemical system, Cascade reaction

Abstract: Enzyme cascade reactions play significant roles in bioelectrochemical processes because they permit more complex reactions. Co-immobilization of multienzyme on the electrode could help to facilitate substrate/intermediate transfer among different enzymes and electron transfer from enzyme active sites to the electrode with high stability and retrievability. Different co-immobilization strategies to construct multienzyme bioelectrodes have been widely reported, however, up to now, they have barely been reviewed. In this review, we focus on recent state-of-the-art techniques for constructing co-immobilized multienzyme electrodes including random and positional co-immobilization. Particular attention is given to strategies such as multienzyme complex and surface display. Cofactor co-immobilization on the electrode is also crucial for the enhancement of catalytic reaction and electron transfer, yet, few studies have been reported. The up-to-date advances in bioelectrochemical applications of multienzyme bioelectrodes are also presented. Finally, key challenges and future perspectives are discussed.

Key words: Multienzyme co-immobilization, Bioelectrode, Bioelectrochemical system, Cascade reaction