Cell-free synthetic biology in the new era of enzyme engineering

doi:10.1016/j.cjche.2020.05.037

中国化学工程学报 ›› 2020, Vol. 28 ›› Issue (11): 2810-2816.DOI: 10.1016/j.cjche.2020.05.037

• Special Topic: Biocatalysis • 上一篇下一篇

Cell-free synthetic biology in the new era of enzyme engineering

Nan Jiang^1,2, Lianju Ma¹, Yuan Lu²

1 College of Life Sciences, Shenyang Normal University, Shenyang 110034, China;
2 Key Lab of Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China

收稿日期:2020-02-21 修回日期:2020-05-30 出版日期:2020-11-28 发布日期:2020-12-31
通讯作者: Lianju Ma, Yuan Lu
基金资助:
This work was supported by the National Key R&D Program of China (2018YFA0901700), the National Natural Science Foundation of China (Nos. 21878173, 21706144), and the Natural Science Foundation of Beijing City (2192023).

Cell-free synthetic biology in the new era of enzyme engineering

Nan Jiang^1,2, Lianju Ma¹, Yuan Lu²

1 College of Life Sciences, Shenyang Normal University, Shenyang 110034, China;
2 Key Lab of Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China

Received:2020-02-21 Revised:2020-05-30 Online:2020-11-28 Published:2020-12-31
Contact: Lianju Ma, Yuan Lu
Supported by:
This work was supported by the National Key R&D Program of China (2018YFA0901700), the National Natural Science Foundation of China (Nos. 21878173, 21706144), and the Natural Science Foundation of Beijing City (2192023).

摘要/Abstract

摘要： With the gradual rise of enzyme engineering, it has played an essential role in synthetic biology, medicine, and biomanufacturing. However, due to the limitation of the cell membrane, the complexity of cellular metabolism, the difficulty of controlling the reaction environment, and the toxicity of some metabolic products in traditional in vivo enzyme engineering, it is usually problematic to express functional enzymes and produce a high yield of synthesized compounds. Recently, cell-free synthetic biology methods for enzyme engineering have been proposed as alternative strategies. This cell-free method has no limitation of the cell membrane and no need to maintain cell viability, and each biosynthetic pathway is highly flexible. This property makes cell-free approaches suitable for the production of valuable products such as functional enzymes and chemicals that are difficult to synthesize. This article aims to discuss the latest advances in cell-free enzyme engineering, assess the trend of this developing topical filed, and analyze its prospects.

关键词: Cell-free synthetic biology, Enzyme engineering, Metabolic engineering

Abstract: With the gradual rise of enzyme engineering, it has played an essential role in synthetic biology, medicine, and biomanufacturing. However, due to the limitation of the cell membrane, the complexity of cellular metabolism, the difficulty of controlling the reaction environment, and the toxicity of some metabolic products in traditional in vivo enzyme engineering, it is usually problematic to express functional enzymes and produce a high yield of synthesized compounds. Recently, cell-free synthetic biology methods for enzyme engineering have been proposed as alternative strategies. This cell-free method has no limitation of the cell membrane and no need to maintain cell viability, and each biosynthetic pathway is highly flexible. This property makes cell-free approaches suitable for the production of valuable products such as functional enzymes and chemicals that are difficult to synthesize. This article aims to discuss the latest advances in cell-free enzyme engineering, assess the trend of this developing topical filed, and analyze its prospects.

Key words: Cell-free synthetic biology, Enzyme engineering, Metabolic engineering

Nan Jiang, Lianju Ma, Yuan Lu. Cell-free synthetic biology in the new era of enzyme engineering[J]. 中国化学工程学报, 2020, 28(11): 2810-2816.

Nan Jiang, Lianju Ma, Yuan Lu. Cell-free synthetic biology in the new era of enzyme engineering[J]. Chinese Journal of Chemical Engineering, 2020, 28(11): 2810-2816.

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Cell-free synthetic biology in the new era of enzyme engineering

Cell-free synthetic biology in the new era of enzyme engineering

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