SCI和EI收录∣中国化工学会会刊

中国化学工程学报 ›› 2021, Vol. 29 ›› Issue (2): 62-73.DOI: 10.1016/j.cjche.2020.12.015

• Synthetic Biotechnology and Metabolic Engineering • 上一篇    下一篇

Engineered microorganisms and enzymes for efficiently synthesizing plant natural products

Wenqiang Li, Wentao Sun, Chun Li   

  1. Key Lab for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China
  • 收稿日期:2020-10-14 修回日期:2020-12-13 出版日期:2021-02-28 发布日期:2021-05-15
  • 通讯作者: Chun Li
  • 基金资助:
    This work was supported by the National Key Research and Development Program of China (2018YFA0901800), and the National Natural Science Foundation of China (No. 21736002).

Engineered microorganisms and enzymes for efficiently synthesizing plant natural products

Wenqiang Li, Wentao Sun, Chun Li   

  1. Key Lab for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China
  • Received:2020-10-14 Revised:2020-12-13 Online:2021-02-28 Published:2021-05-15
  • Contact: Chun Li
  • Supported by:
    This work was supported by the National Key Research and Development Program of China (2018YFA0901800), and the National Natural Science Foundation of China (No. 21736002).

摘要: Plant natural products are a kind of active substance widely used in pharmaceuticals and foods. However, the current production mode based on plant culture and extraction suffer complex processes and severe concerns for environmental and ecological. With the increasing awareness of environmental sustainability, engineered microbial cell factories have been an alternative approach to produce natural products. Many engineering strategies have been utilized in microbial biosynthesis of complex phytochemicals such as dynamic control and substructure engineering. Meanwhile, Enzyme engineering including directed evolution and rational design has been implemented to improve enzyme catalysis efficiency and stability as well as change promiscuity to expand product spectra. In this review, we discussed recent advances in microbial biosynthesis of complex phytochemicals from the following aspects, including pathway construction, strain engineering to boost the production.

关键词: Plant natural products, Microorganisms, Synthetic biology, Enzyme engineering

Abstract: Plant natural products are a kind of active substance widely used in pharmaceuticals and foods. However, the current production mode based on plant culture and extraction suffer complex processes and severe concerns for environmental and ecological. With the increasing awareness of environmental sustainability, engineered microbial cell factories have been an alternative approach to produce natural products. Many engineering strategies have been utilized in microbial biosynthesis of complex phytochemicals such as dynamic control and substructure engineering. Meanwhile, Enzyme engineering including directed evolution and rational design has been implemented to improve enzyme catalysis efficiency and stability as well as change promiscuity to expand product spectra. In this review, we discussed recent advances in microbial biosynthesis of complex phytochemicals from the following aspects, including pathway construction, strain engineering to boost the production.

Key words: Plant natural products, Microorganisms, Synthetic biology, Enzyme engineering