SCI和EI收录∣中国化工学会会刊

中国化学工程学报 ›› 2022, Vol. 45 ›› Issue (5): 238-247.DOI: 10.1016/j.cjche.2021.03.020

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Fabrication and characterization of hierarchical porous Ni2+ doped hydroxyapatite microspheres and their enhanced protein adsorption capacity

Yaling Li, Hao Ai, Liangzhi Qiao, Yinghong Wang, Kaifeng Du   

  1. Department of Pharmaceutical & Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu 610065, China
  • 收稿日期:2020-12-28 修回日期:2021-03-25 出版日期:2022-05-28 发布日期:2022-06-22
  • 通讯作者: Kaifeng Du,E-mail:kfdu@scu.edu.cn
  • 基金资助:
    The work was funded by the National Natural Science Foundation of China (21676170).

Fabrication and characterization of hierarchical porous Ni2+ doped hydroxyapatite microspheres and their enhanced protein adsorption capacity

Yaling Li, Hao Ai, Liangzhi Qiao, Yinghong Wang, Kaifeng Du   

  1. Department of Pharmaceutical & Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu 610065, China
  • Received:2020-12-28 Revised:2021-03-25 Online:2022-05-28 Published:2022-06-22
  • Contact: Kaifeng Du,E-mail:kfdu@scu.edu.cn
  • Supported by:
    The work was funded by the National Natural Science Foundation of China (21676170).

摘要: Hydroxyapatite (HAP) is a common bio-adsorbent, which performance depends heavily upon its morphology and microporous structure. In this study, a novel synthesis strategy was proposed for hierarchical porous HAP microspheres by a simple “one-pot” hydrothermal reaction. In the strategy, L-glutamic acid serves as soft template to modulate the morphology and inner crystalline of HAP. To evaluate the application potential, doping Ni2+ on hierarchical porous HAP microspheres gives metal chelated affinity adsorbents. The prepared adsorbents show a perfect spherical shape, particles size of 96.6 μm, relatively specific surface area of 48.5 m2·g-1 and hierarchical pores (mesopores: 4 nm and macropores: 53 nm). By the adsorption evaluation, it reveals that the Ni2+-HAP adsorbents have high adsorption capacities of 275.11 and 97.55 m2·g-1 for hemoglobin and bovine serum albumin, respectively, which is comparable to other similar adsorbent. Therefore, this work provides a promising method for high-efficiency hydroxyapatite microspheres for proteins purification.

关键词: Hierarchical porous hydroxyapatite microspheres, Templates, Metal doping, Proteins adsorption

Abstract: Hydroxyapatite (HAP) is a common bio-adsorbent, which performance depends heavily upon its morphology and microporous structure. In this study, a novel synthesis strategy was proposed for hierarchical porous HAP microspheres by a simple “one-pot” hydrothermal reaction. In the strategy, L-glutamic acid serves as soft template to modulate the morphology and inner crystalline of HAP. To evaluate the application potential, doping Ni2+ on hierarchical porous HAP microspheres gives metal chelated affinity adsorbents. The prepared adsorbents show a perfect spherical shape, particles size of 96.6 μm, relatively specific surface area of 48.5 m2·g-1 and hierarchical pores (mesopores: 4 nm and macropores: 53 nm). By the adsorption evaluation, it reveals that the Ni2+-HAP adsorbents have high adsorption capacities of 275.11 and 97.55 m2·g-1 for hemoglobin and bovine serum albumin, respectively, which is comparable to other similar adsorbent. Therefore, this work provides a promising method for high-efficiency hydroxyapatite microspheres for proteins purification.

Key words: Hierarchical porous hydroxyapatite microspheres, Templates, Metal doping, Proteins adsorption