SCI和EI收录∣中国化工学会会刊

Chinese Journal of Chemical Engineering ›› 2012, Vol. 20 ›› Issue (5): 995-1002.

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Isolation and Characterization of Heterotrophic Nitrifying Strain W1*

吕永康1, 王荀1, 刘博凯2, 刘玉香3, 杨晓华1   

  1. 1. Key Laboratory of Coal Science and Technology, Taiyuan University of Technology, Taiyuan 030024, China;
    2. Engineering & the Built Environment (EBE), University of Cape Town, Cape Town7701, South Africa;
    3. College of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, China
  • 收稿日期:2011-06-20 修回日期:2011-12-12 出版日期:2012-10-28 发布日期:2012-11-06
  • 通讯作者: LÜ Yongkang,E-mail:lykang@tyut.edu.cn
  • 基金资助:
    Supported by the National Natural Science Foundation of China (51078252);the International Cooperation Projects of Shanxi Province (2010081018);the Natural Science Foundation of Shanxi Province (2010011016-1)

Isolation and Characterization of Heterotrophic Nitrifying Strain W1*

LÜ Yongkang1, WANG Xun1, LIU Bokai2, LIU Yuxiang3, YANG Xiaohua1   

  1. 1. Key Laboratory of Coal Science and Technology, Taiyuan University of Technology, Taiyuan 030024, China;
    2. Engineering & the Built Environment (EBE), University of Cape Town, Cape Town7701, South Africa;
    3. College of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, China
  • Received:2011-06-20 Revised:2011-12-12 Online:2012-10-28 Published:2012-11-06
  • Supported by:
    Supported by the National Natural Science Foundation of China (51078252);the International Cooperation Projects of Shanxi Province (2010081018);the Natural Science Foundation of Shanxi Province (2010011016-1)

摘要: In a high concentration substrate medium, a heterotrophic bacterium with high removal efficiency of ammonium, named W1, was isolated from activated sludge of coking wastewater treatment facility. The bacterium was Gram-negative, rod-shaped, and identified preliminarily as Alcaligenes sp. according to its morphological and physiological properties and its 16S rRNA gene sequence analysis. In the high concentration ammonium medium (400 mg·L-1 NH4--N), the effects of C source, N source, C/N ratio and initial pH of medium on ammonium removal were investigated in order to determine the optimal condition for strain W1. The maximum ammonium removal was around 95% in 4 days in an improved medium. The production of N2 gas was examined in a closed system that was full of pure oxygen at the beginning. N2 gas was detected in the system after 4 days of cultivation, which further testified that strain W1 has heterotrophic nitrification and aerobic denitrification abilities simultaneously.

关键词: heterotrophic nitrification, aerobic de-nitrification, high concentration ammonium, Alcaligenes sp.

Abstract: In a high concentration substrate medium, a heterotrophic bacterium with high removal efficiency of ammonium, named W1, was isolated from activated sludge of coking wastewater treatment facility. The bacterium was Gram-negative, rod-shaped, and identified preliminarily as Alcaligenes sp. according to its morphological and physiological properties and its 16S rRNA gene sequence analysis. In the high concentration ammonium medium (400 mg·L-1 NH4--N), the effects of C source, N source, C/N ratio and initial pH of medium on ammonium removal were investigated in order to determine the optimal condition for strain W1. The maximum ammonium removal was around 95% in 4 days in an improved medium. The production of N2 gas was examined in a closed system that was full of pure oxygen at the beginning. N2 gas was detected in the system after 4 days of cultivation, which further testified that strain W1 has heterotrophic nitrification and aerobic denitrification abilities simultaneously.

Key words: heterotrophic nitrification, aerobic de-nitrification, high concentration ammonium, Alcaligenes sp.