Effective and simple recovery of 1,3-propanediol from a fermented medium by liquid-liquid extraction system with ethanol and K3PO4

doi:10.1016/j.cjche.2017.06.005

Chinese Journal of Chemical Engineering ›› 2018, Vol. 26 ›› Issue (1): 104-108.DOI: 10.1016/j.cjche.2017.06.005

• Separation Science and Engineering • 上一篇下一篇

Effective and simple recovery of 1,3-propanediol from a fermented medium by liquid-liquid extraction system with ethanol and K₃PO₄

Daiana Wischral^1,2, Hongxin Fu², Fernando L. Pellegrini Pessoa³, Nei Pereira Jr¹, Shang-Tian Yang²

1 School of Chemistry, Department of Biochemical Engineering, Federal University of Rio de Janeiro, Av. Horácio Macedo 2030, Bloco E., Rio de Janeiro, RJ 21949-900, Brazil;
2 William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Avenue, Columbus, OH 43210, USA;
3 School of Chemistry, Department of Chemical Engineering, Federal University of Rio de Janeiro, Av. Horácio Macedo 2030, Bloco E., Rio de Janeiro, RJ 21949-900, Brazil

收稿日期:2017-02-08 修回日期:2017-06-06 出版日期:2018-01-28 发布日期:2018-03-01
通讯作者: Daiana Wischral,E-mail address:daiana@eq.ufrj.br

Effective and simple recovery of 1,3-propanediol from a fermented medium by liquid-liquid extraction system with ethanol and K₃PO₄

Daiana Wischral^1,2, Hongxin Fu², Fernando L. Pellegrini Pessoa³, Nei Pereira Jr¹, Shang-Tian Yang²

1 School of Chemistry, Department of Biochemical Engineering, Federal University of Rio de Janeiro, Av. Horácio Macedo 2030, Bloco E., Rio de Janeiro, RJ 21949-900, Brazil;
2 William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Avenue, Columbus, OH 43210, USA;
3 School of Chemistry, Department of Chemical Engineering, Federal University of Rio de Janeiro, Av. Horácio Macedo 2030, Bloco E., Rio de Janeiro, RJ 21949-900, Brazil

Received:2017-02-08 Revised:2017-06-06 Online:2018-01-28 Published:2018-03-01
Contact: Daiana Wischral,E-mail address:daiana@eq.ufrj.br

摘要/Abstract

摘要： 1,3-Propanediol, traditionally obtained from fossils, has numerous industrial applications, including use in the production of high performance polymers. The microbial production of 1,3-propanediol presents several opportunities, and the final purity grade determines its price and commercial viability. The development of novel separation technology could improve the economic viability of the bioproduction of 1,3-propanediol. Thus, we investigated salting-out extraction as a novel process for 1,3-propanediol recovery from fermentation broth. Initially, a screening for the best salt/solvent combination was conducted and then optimized using the response surface methodology. The solvents studied were methanol, ethanol, isopropanol and acetone, and the salts examined were K₂HPO₄, Na₂CO₃, K₂CO₃, (NH₄)₂SO₄, NaHPO₄, K₃PO₄ and C₆H₅NaO₇. The optimal extraction system consisted of 34 wt% K₃PO₄, 28 wt% ethanol, and 38 wt% fermentation broth containing 23.0 g·L^-1 1,3-propanediol, which gave the highest partition coefficient of 33 and recovery yield of 97%. The results demonstrated that salting-out extraction was a promising method for 1,3-propanediol recovery from fermentation broth.

关键词: Fermentation, Downstream process, 1, 3-Propanediol, Clostridium beijerinckii, Liquid-liquid extraction, Salting-out extraction

Abstract: 1,3-Propanediol, traditionally obtained from fossils, has numerous industrial applications, including use in the production of high performance polymers. The microbial production of 1,3-propanediol presents several opportunities, and the final purity grade determines its price and commercial viability. The development of novel separation technology could improve the economic viability of the bioproduction of 1,3-propanediol. Thus, we investigated salting-out extraction as a novel process for 1,3-propanediol recovery from fermentation broth. Initially, a screening for the best salt/solvent combination was conducted and then optimized using the response surface methodology. The solvents studied were methanol, ethanol, isopropanol and acetone, and the salts examined were K₂HPO₄, Na₂CO₃, K₂CO₃, (NH₄)₂SO₄, NaHPO₄, K₃PO₄ and C₆H₅NaO₇. The optimal extraction system consisted of 34 wt% K₃PO₄, 28 wt% ethanol, and 38 wt% fermentation broth containing 23.0 g·L^-1 1,3-propanediol, which gave the highest partition coefficient of 33 and recovery yield of 97%. The results demonstrated that salting-out extraction was a promising method for 1,3-propanediol recovery from fermentation broth.

Key words: Fermentation, Downstream process, 1,3-Propanediol, Clostridium beijerinckii, Liquid-liquid extraction, Salting-out extraction

Daiana Wischral, Hongxin Fu, Fernando L. Pellegrini Pessoa, Nei Pereira Jr, Shang-Tian Yang. Effective and simple recovery of 1,3-propanediol from a fermented medium by liquid-liquid extraction system with ethanol and K₃PO₄[J]. Chinese Journal of Chemical Engineering, 2018, 26(1): 104-108.

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Effective and simple recovery of 1,3-propanediol from a fermented medium by liquid-liquid extraction system with ethanol and K₃PO₄

Effective and simple recovery of 1,3-propanediol from a fermented medium by liquid-liquid extraction system with ethanol and K₃PO₄

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