Thermostable ethanol tolerant xylanase from a cold-adapted marine species Acinetobacter johnsonii

doi:10.1016/j.cjche.2018.06.019

中国化学工程学报 ›› 2019, Vol. 27 ›› Issue (5): 1166-1170.DOI: 10.1016/j.cjche.2018.06.019

• Biotechnology and Bioengineering • 上一篇下一篇

Thermostable ethanol tolerant xylanase from a cold-adapted marine species Acinetobacter johnsonii

Dongsheng Xue¹, Xuhao Zeng¹, Dongqiang Lin², Shanjing Yao²

1 Key Laboratory of Fermentation Engineering(Hubei Universty of Technology), Ministry of Education, Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology, Wuhan 430068, China;
2 Department of Chemical and Bioengineering, Zhejiang University, Hangzhou 310027, China

收稿日期:2018-03-30 修回日期:2018-05-19 出版日期:2019-05-28 发布日期:2019-06-27
通讯作者: Shanjing Yao
基金资助:
Supported by the Science and Technology Research Project of Hubei Provincial Department of Education (B2017041).

Thermostable ethanol tolerant xylanase from a cold-adapted marine species Acinetobacter johnsonii

Dongsheng Xue¹, Xuhao Zeng¹, Dongqiang Lin², Shanjing Yao²

1 Key Laboratory of Fermentation Engineering(Hubei Universty of Technology), Ministry of Education, Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology, Wuhan 430068, China;
2 Department of Chemical and Bioengineering, Zhejiang University, Hangzhou 310027, China

Received:2018-03-30 Revised:2018-05-19 Online:2019-05-28 Published:2019-06-27
Contact: Shanjing Yao
Supported by:
Supported by the Science and Technology Research Project of Hubei Provincial Department of Education (B2017041).

摘要/Abstract

摘要： A xylanase-producing bacterium, isolated from deep sea sediments, was identified as the cold-adapted marine species Acinetobacter Johnsonii. A cold-adapted marine species Acinetobacter Johnsonii could grow at 4℃. The optimum temperature and pH of xylanase from a cold-adapted marine species Acinetobacter Johnsonii were 55℃ and pH 6.0. Xylanase from a cold-adapted marine species Acinetobacter Johnsonii remained at 80% activity after incubation for 1 h at 65℃. The xylanase activity was 1.2-fold higher in 4% ethanol solution than in ethanol free solution. Gibbs free energy of denaturation, ΔG, was higher in 4% ethanol solution than in ethanol free solution. Thermostable ethanol tolerant xylanase was valuable for bioethanol production by simultaneous saccharification and fermentation process with xylan as a carbon source.

关键词: Xylanase, Ethanol tolerant, Thermostable, Cold-adapted, Acinetobacter Johnsonii

Abstract: A xylanase-producing bacterium, isolated from deep sea sediments, was identified as the cold-adapted marine species Acinetobacter Johnsonii. A cold-adapted marine species Acinetobacter Johnsonii could grow at 4℃. The optimum temperature and pH of xylanase from a cold-adapted marine species Acinetobacter Johnsonii were 55℃ and pH 6.0. Xylanase from a cold-adapted marine species Acinetobacter Johnsonii remained at 80% activity after incubation for 1 h at 65℃. The xylanase activity was 1.2-fold higher in 4% ethanol solution than in ethanol free solution. Gibbs free energy of denaturation, ΔG, was higher in 4% ethanol solution than in ethanol free solution. Thermostable ethanol tolerant xylanase was valuable for bioethanol production by simultaneous saccharification and fermentation process with xylan as a carbon source.

Key words: Xylanase, Ethanol tolerant, Thermostable, Cold-adapted, Acinetobacter Johnsonii

Dongsheng Xue, Xuhao Zeng, Dongqiang Lin, Shanjing Yao. Thermostable ethanol tolerant xylanase from a cold-adapted marine species Acinetobacter johnsonii[J]. 中国化学工程学报, 2019, 27(5): 1166-1170.

Dongsheng Xue, Xuhao Zeng, Dongqiang Lin, Shanjing Yao. Thermostable ethanol tolerant xylanase from a cold-adapted marine species Acinetobacter johnsonii[J]. Chinese Journal of Chemical Engineering, 2019, 27(5): 1166-1170.

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Thermostable ethanol tolerant xylanase from a cold-adapted marine species Acinetobacter johnsonii

Thermostable ethanol tolerant xylanase from a cold-adapted marine species Acinetobacter johnsonii

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