Preparation and characterization of the n-HA/PVA/CS porous composite hydrogel

doi:10.1016/j.cjche.2019.01.029

中国化学工程学报 ›› 2020, Vol. 28 ›› Issue (2): 598-602.DOI: 10.1016/j.cjche.2019.01.029

• Materials and Product Engineering • 上一篇下一篇

Preparation and characterization of the n-HA/PVA/CS porous composite hydrogel

Wei Liang, Zhongkuan Luo, Li Zhou

College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen 518055, China

收稿日期:2017-12-26 修回日期:2019-01-13 出版日期:2020-02-28 发布日期:2020-05-21
通讯作者: Li Zhou
基金资助:
Supported by the Key Technology R&D Program of Shenzhen Municipal (JSGG20120614164013545) and Basic Research Program of Shenzhen Municipal (JCYJ20130329102614715).

Preparation and characterization of the n-HA/PVA/CS porous composite hydrogel

Wei Liang, Zhongkuan Luo, Li Zhou

College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen 518055, China

Received:2017-12-26 Revised:2019-01-13 Online:2020-02-28 Published:2020-05-21
Contact: Li Zhou
Supported by:
Supported by the Key Technology R&D Program of Shenzhen Municipal (JSGG20120614164013545) and Basic Research Program of Shenzhen Municipal (JCYJ20130329102614715).

摘要/Abstract

摘要： In this paper, a new method combines chemical/physical crosslinking, and emulsification-foaming porogenic was adopted to prepare n-hydroxyapatite (n-HA)/polyvinyl alcohol (PVA)/chitosan (CS) porous composite hydrogel using artificial cornea scaffold materials. The fabricate conditions, including the type and amount of emulsification-foaming porogen, mixing time and speed etc. were researched. The results showed the optimal condition that the alkylphenol polyoxyethylene ether (OP) acted as emulsification-foaming porogen, with the ratio of W_PVA/W_OP as 3.75, and mixing 15 min with a stirring speed of 800 r·min^-1. Additionally, the fabricated composite hydrogel scaffold materials possessed interconnected internal holes, a moisture content of above 65%, and tensile strength of above 6 MPa. In vitro cytotoxicity and acute systemic toxicity assay confirmed that the scaffolds did not show any cytotoxicity. The as-prepared hydrogel could be a promising candidate for artificial cornea scaffold material.

关键词: Porous composite hydrogel, Chemical/physical crosslinking, Emulsification-foaming porogen, Polyvinyl alcohol, Biocompatibility, Artificial cornea scaffold

Abstract: In this paper, a new method combines chemical/physical crosslinking, and emulsification-foaming porogenic was adopted to prepare n-hydroxyapatite (n-HA)/polyvinyl alcohol (PVA)/chitosan (CS) porous composite hydrogel using artificial cornea scaffold materials. The fabricate conditions, including the type and amount of emulsification-foaming porogen, mixing time and speed etc. were researched. The results showed the optimal condition that the alkylphenol polyoxyethylene ether (OP) acted as emulsification-foaming porogen, with the ratio of W_PVA/W_OP as 3.75, and mixing 15 min with a stirring speed of 800 r·min^-1. Additionally, the fabricated composite hydrogel scaffold materials possessed interconnected internal holes, a moisture content of above 65%, and tensile strength of above 6 MPa. In vitro cytotoxicity and acute systemic toxicity assay confirmed that the scaffolds did not show any cytotoxicity. The as-prepared hydrogel could be a promising candidate for artificial cornea scaffold material.

Key words: Porous composite hydrogel, Chemical/physical crosslinking, Emulsification-foaming porogen, Polyvinyl alcohol, Biocompatibility, Artificial cornea scaffold

Wei Liang, Zhongkuan Luo, Li Zhou. Preparation and characterization of the n-HA/PVA/CS porous composite hydrogel[J]. 中国化学工程学报, 2020, 28(2): 598-602.

Wei Liang, Zhongkuan Luo, Li Zhou. Preparation and characterization of the n-HA/PVA/CS porous composite hydrogel[J]. Chinese Journal of Chemical Engineering, 2020, 28(2): 598-602.

参考文献

[1] H. Bai, L. Wang, Y. Huang, Histopathological observation of new soft one-piece keratoprosthesis skirt implanted into alkali burned rabbit corneas, Chinese J Tissue Eng Res 17(34) (2013) 6138-6143.
[2] Y. Wang, C. Xing, X. Zhou, X. Zhou, L. Wang, H.G. Han, Classification, surface modification and biocompatibility of artificial cornea materials, Chinese J Tissue Eng Res 16(16) (2012) 2989-2996.
[3] J. Xiong, Y.P. Deng, Y.S. Wang, Y.B. Li, F.L. Xu, The biological response of rabbit cornea implanted with nano-HA/PVA composite hydrogel keratoprosthesis, J Ophthalmol 20(3) (2011) 170-175.
[4] T. Yan, R. Sun, H. Deng, B.H. Tan, N.J. Ao, Attachment, morphology and biomechanical changes of keratocytes cultured on unmodified and modified p(HEMA-MMA) hydrogel, J Clin Rehabil Tissue 29(3) (2010) 232-236.
[5] B. Bachmann, G. Avgitidou, S. Siebelmann, C. Cursiefen, Pediatric corneal surgery and corneal transplantation, Ophthalmologe. 112(2) (2015) 110.
[6] T.V. Chirila, S. Vijayasekaran, R. Horne, Robert Horne, Y.C. Chen, P.D. Dalton, I.J. Constable, G.J. Crawford, Interpenetrating polymer network (IPN) as a permanent joint between the elements of a new type of artificial cornea, J Biomed Mater Res 28(6) (1994) 745-753.
[7] M. Yar, S. Shahzad, S.A. Siddiqi, N. Mahmood, A. Rauf, M.S. Anwar, A.A. Chaudhry, I.U. Rehman, Triethyl orthoformate mediated a novel crosslinking method for the preparation of hydrogels for tissue engineering applications:characterization and in vitro cytocompatibility analysis, Mat Sci Eng C-Mater 56(2015) 154-164.
[8] A.M. Ionescu, M. Alaminos, Cardona J. De La Cruz, J.D.G.L. Duran, M. GonzalezAndrades, R. Ghinea, A. Campos, E. Hita, M.D. Perez, Investigating a novel nanostructured fibrin-agarose biomaterial for human cornea tissue engineering:Rheological properties, J Mech Behav Biomed 4(8) (2011) 1963-1973.
[9] A.M. Oelker, M.W. Grinstaff, Synthesis, characterization, and in vitro evaluation of a hydrogel-based corneal onlay, Ieee T Nanobiosci 11(1) (2012) 37-45.
[10] K. Tonsomboon, M.L. Oyen, Composite electrospun gelatin fiber-alginate gel scaffolds for mechanically robust tissue engineered cornea, J Mech Behav Biomed 21(2013) 185-194.
[11] S.J. Liu, D.L. Zhou, D.P. Liu, X. Zang, Q. Long, D.L. Chen, Preparation and properties of PVA/CS porous hydrogen, J Liaoning Me U 34(2) (2013) 71-74103-104.
[12] M. He, Y. Guoqiang, P. Wang, Progress of porous hydrogels, Chem Ind Eng 28(9) (2009) 1578-1582.
[13] J. Hu, X. Wang, S. Zheng, Aggregation of gels induced by microscopic supramolecular complexation, Polym Mater Sci Eng 28(9) (2012) 72-75.
[14] G.D. Lu, Q.Z. Yan, X.T. Su, Z.Q. Liu, C.C. Ge, Progress of porous hydrogels, Prog Chem 19(4) (2007) 485-493.
[15] F. Luo, The study on biomedical materials of hydrogel, Guangdong Chem Ind 38(8) (2011) 104-105(in Chinese).
[16] D. Hao, E. Xie, B. Chen, Study on preparation of repair of spinal cord injury with hydrogel, Orthop Biomech Mater Clin Study 10(5) (2013) 9-12,66.
[17] A. Islam, T. Yasin, N. Gull, Khan, M.S. Shahzad, M. Aneela, A.S. Muhammad, J. Muhammad, R. Tahir, H. Muhammad, Fabrication and performance characteristics of tough hydrogel scaffolds based on biocompatible polymers, Int J BiolMacroMol 92(2016) 1-10.
[18] International Organization for Standardization, Biological evaluation of medical devices-Part 5:Tests for in vitro cytotoxicity, ISO 10993-5:2009, 2017.
[19] International Organization for Standardization, Biological evaluation of medical devices-Part 11:Tests for systemic toxicity, ISO 10993-11:1993, 2017.
[20] S. Yang, P. Lei, Y. Shan, Y.J. Shan, D. Zhang, Preparation and characterization of antibacterial electrospun chitosan/poly (vinyl alcohol)/graphene oxide composite nanofibrous membrane, Appl Surf Sci 435(2018) 832-840.
[21] M.D. Figueroa-Pizano, I. Velaz, F.J. Penas, P. Zavala-Rivera, A.J. Rosas-Durazo, A.D. Maldonado-Arce, M.E. Martinez-Barbosa, Effect of freeze-thawing conditions for preparation of chitosan-poly (vinyl alcohol) hydrogels and drug release studies, Carbohydr Polym 195(2018) 476-485.
[22] R. Feng, R. Fu, Z.G. Duan, C.H. Zhu, X.X. Ma, D.D. Fan, X. Li, Preparation of sponge-like macroporous PVA hydrogels via n-HA enhanced phase separation and their potential as wound dressing, J Biommat Sci-Polym 29(12) (2018) 1463-1481.
[23] D. Anandan, S.M. Stella, N.A. Nambiraj, U. Vijayalakshmi, A.K. Jaiswal, Development of mechanically compliant 3D composite scaffolds for bone tissue engineering applications, J Biomed Mater Res A 106(12) (2018) 3267-3274.

Preparation and characterization of the n-HA/PVA/CS porous composite hydrogel

Preparation and characterization of the n-HA/PVA/CS porous composite hydrogel

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