Immobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradation

doi:10.1016/j.cjche.2019.12.025

中国化学工程学报 ›› 2020, Vol. 28 ›› Issue (4): 1136-1144.DOI: 10.1016/j.cjche.2019.12.025

• Biotechnology and Bioengineering • 上一篇下一篇

Immobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradation

Jelena Bebić¹, Katarina Banjanac^1,2, Marija Ćorović³, Ana Milivojević², Milica Simović³, Aleksandar Marinković⁴, Dejan Bezbradica³

1 Directorate of Measures and Precious Metals, Mike Alasa 14, 11000 Belgrade, Serbia;
2 Innovation Centre of Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia;
3 Department of Biochemical Engineering and Biotechnology, Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia;
4 Department of Organic Chemistry, Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia

收稿日期:2019-09-28 修回日期:2019-12-19 出版日期:2020-04-28 发布日期:2020-07-27
通讯作者: Jelena Bebić, Katarina Banjanac, Marija Ćorović, Ana Milivojević, Milica Simović, Aleksandar Marinković, Dejan Bezbradica
基金资助:
Authors are grateful to the Ministry of Education, Science and Technological Development, Republic of Serbia, within projects Ⅲ 46010, Ⅲ 45019 and TR31035 for the financial, and to Directorate of Measures and Precious Metals, Ministry of Economy, Republic of Serbia for the technical support. We thank Dr. Pavle Andrić (Novozymes A/S, Bagsvaerd, Denmark) for providing commercial laccase from M. thermophila expressed in A. oryzae (Novozym^® 51003).

Immobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradation

Jelena Bebić¹, Katarina Banjanac^1,2, Marija Ćorović³, Ana Milivojević², Milica Simović³, Aleksandar Marinković⁴, Dejan Bezbradica³

1 Directorate of Measures and Precious Metals, Mike Alasa 14, 11000 Belgrade, Serbia;
2 Innovation Centre of Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia;
3 Department of Biochemical Engineering and Biotechnology, Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia;
4 Department of Organic Chemistry, Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia

Received:2019-09-28 Revised:2019-12-19 Online:2020-04-28 Published:2020-07-27
Contact: Jelena Bebić, Katarina Banjanac, Marija Ćorović, Ana Milivojević, Milica Simović, Aleksandar Marinković, Dejan Bezbradica
Supported by:
Authors are grateful to the Ministry of Education, Science and Technological Development, Republic of Serbia, within projects Ⅲ 46010, Ⅲ 45019 and TR31035 for the financial, and to Directorate of Measures and Precious Metals, Ministry of Economy, Republic of Serbia for the technical support. We thank Dr. Pavle Andrić (Novozymes A/S, Bagsvaerd, Denmark) for providing commercial laccase from M. thermophila expressed in A. oryzae (Novozym^® 51003).

摘要/Abstract

摘要： This work is focused on immobilization of laccase from Myceliophthora thermophila expressed in Aspergillus oryzae (Novozym 51003^® laccase) on amino modified fumed nano-silica (AFNS) and the possible use in bioremediation. Hereby, for the first time, factors affecting the immobilization of Novozym 51003^® laccase on AFNS were investigated for defining the immobilization mechanism and optimizing the utilization of AFNS as support for laccase immobilization. The highest specific activity (13.1 IU·mg^-1 proteins) was achieved at offered 160 mg per g of AFNS and for the same offered protein concentration the highest activity immobilization yield, reaching 68.3% after the equilibrium time, at optimum pH 5.0, was obtained. Laccase immobilization occurs by adsorption as monolayer enzyme binding in 40 min, following pseudo-first-order kinetics. The possible use of obtained immobilized preparation was investigated in degradation of pesticide lindane. Within 24 h, lindane concentration was reduced to 56.8% of initial concentration and after seven repeated reuses it retained 70% of the original activity.

关键词: Silica nanoparticles, Laccase, Enzyme immobilization, Lindane, Bioremediation

Abstract: This work is focused on immobilization of laccase from Myceliophthora thermophila expressed in Aspergillus oryzae (Novozym 51003^® laccase) on amino modified fumed nano-silica (AFNS) and the possible use in bioremediation. Hereby, for the first time, factors affecting the immobilization of Novozym 51003^® laccase on AFNS were investigated for defining the immobilization mechanism and optimizing the utilization of AFNS as support for laccase immobilization. The highest specific activity (13.1 IU·mg^-1 proteins) was achieved at offered 160 mg per g of AFNS and for the same offered protein concentration the highest activity immobilization yield, reaching 68.3% after the equilibrium time, at optimum pH 5.0, was obtained. Laccase immobilization occurs by adsorption as monolayer enzyme binding in 40 min, following pseudo-first-order kinetics. The possible use of obtained immobilized preparation was investigated in degradation of pesticide lindane. Within 24 h, lindane concentration was reduced to 56.8% of initial concentration and after seven repeated reuses it retained 70% of the original activity.

Key words: Silica nanoparticles, Laccase, Enzyme immobilization, Lindane, Bioremediation

Jelena Bebić, Katarina Banjanac, Marija Ćorović, Ana Milivojević, Milica Simović, Aleksandar Marinković, Dejan Bezbradica. Immobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradation[J]. 中国化学工程学报, 2020, 28(4): 1136-1144.

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Immobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradation

Immobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradation

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