SCI和EI收录∣中国化工学会会刊

中国化学工程学报 ›› 2020, Vol. 28 ›› Issue (8): 2152-2159.DOI: 10.1016/j.cjche.2020.02.028

• Biotechnology and Bioengineering • 上一篇    下一篇

Immobilized laccase on magnetic nanoparticles for enhanced lignin model compounds degradation

Xinyan Chen1, Bin He1, Mi Feng1, Dingwei Zhao1, Jian Sun1,2   

  1. 1 Key Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of Technology, Beijing 100081, China;
    2 Advanced Research Institute of Multidisciplinary Science, Beijing Institute of Technology, Beijing 100081, China
  • 收稿日期:2020-02-03 修回日期:2020-02-28 出版日期:2020-08-28 发布日期:2020-09-19
  • 通讯作者: Jian Sun
  • 基金资助:
    This work was supported by the Startup Foundation of Beijing Institute of Technology, China(3160011181808).

Immobilized laccase on magnetic nanoparticles for enhanced lignin model compounds degradation

Xinyan Chen1, Bin He1, Mi Feng1, Dingwei Zhao1, Jian Sun1,2   

  1. 1 Key Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of Technology, Beijing 100081, China;
    2 Advanced Research Institute of Multidisciplinary Science, Beijing Institute of Technology, Beijing 100081, China
  • Received:2020-02-03 Revised:2020-02-28 Online:2020-08-28 Published:2020-09-19
  • Contact: Jian Sun
  • Supported by:
    This work was supported by the Startup Foundation of Beijing Institute of Technology, China(3160011181808).

摘要: As a natural aromatic polymer, lignin has great potential but limited industrial application due to its complex chemical structure. Among strategies for lignin conversion, biodegradation has attracted promising interest recently in term of efficiency, selectivity and mild condition. In order to overcome the issues of poor stability and non-reusability of enzyme in the biodegradation of lignin, this work explored a protocol of immobilized laccase on magnetic nanoparticles (MNPs) with rough surfaces for enhanced lignin model compounds degradation. Scanning electron microscope with energy dispersive spectrometer (SEM-EDS), flourier transformation infrared spectroscopy (FTIR) and thermal gravimetric analysis (TGA) were utilized to characterize the immobilization of laccase. The results showed a maximum activity recovery of 64.7% towards laccase when it was incubated with MNPs and glutaraldehyde (GA) with concentrations of 6 mg·ml-1 and 7.5 mg·ml-1 for 5 h, respectively. The immobilized laccase showed improved thermal stability and pH tolerance compared with free laccase, and remained more than 80% of its initial activity after 20 days of storage at 4 ℃. In addition, about 40% residual activity of the laccase remained after 8 times cycles. Gas chromatography-mass spectrometry (GC-MS) was utilized to characterize the products of lignin model compound degradation and activation, and the efficiency of immobilized laccase was calculated to be 1-5 times that of free laccase. It was proposed that the synergistic effect between MNPs and laccase displays an important role in the enhancement of stability and activity in lignin model compound biodegradation.

关键词: Laccase, Immobilization, Magnetic nanoparticles, Lignin model compounds, Degradation

Abstract: As a natural aromatic polymer, lignin has great potential but limited industrial application due to its complex chemical structure. Among strategies for lignin conversion, biodegradation has attracted promising interest recently in term of efficiency, selectivity and mild condition. In order to overcome the issues of poor stability and non-reusability of enzyme in the biodegradation of lignin, this work explored a protocol of immobilized laccase on magnetic nanoparticles (MNPs) with rough surfaces for enhanced lignin model compounds degradation. Scanning electron microscope with energy dispersive spectrometer (SEM-EDS), flourier transformation infrared spectroscopy (FTIR) and thermal gravimetric analysis (TGA) were utilized to characterize the immobilization of laccase. The results showed a maximum activity recovery of 64.7% towards laccase when it was incubated with MNPs and glutaraldehyde (GA) with concentrations of 6 mg·ml-1 and 7.5 mg·ml-1 for 5 h, respectively. The immobilized laccase showed improved thermal stability and pH tolerance compared with free laccase, and remained more than 80% of its initial activity after 20 days of storage at 4 ℃. In addition, about 40% residual activity of the laccase remained after 8 times cycles. Gas chromatography-mass spectrometry (GC-MS) was utilized to characterize the products of lignin model compound degradation and activation, and the efficiency of immobilized laccase was calculated to be 1-5 times that of free laccase. It was proposed that the synergistic effect between MNPs and laccase displays an important role in the enhancement of stability and activity in lignin model compound biodegradation.

Key words: Laccase, Immobilization, Magnetic nanoparticles, Lignin model compounds, Degradation