Immobilization of Papain in Biosilica Matrix and Its Catalytic Property

doi:10.1016/S1004-9541(13)60528-5

Chinese Journal of Chemical Engineering ›› 2013, Vol. 21 ›› Issue (6): 670-675.DOI: 10.1016/S1004-9541(13)60528-5

Immobilization of Papain in Biosilica Matrix and Its Catalytic Property

周丽亚¹, 王翠¹, 姜艳军^1,2, 高静¹

1 School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130, China;
2 National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China

收稿日期:2012-06-25 修回日期:2012-10-20 出版日期:2013-06-28 发布日期:2013-07-03
通讯作者: GAO Jing
基金资助:
Supported by the National Natural Science Foundation of China (21006020, 21276060, 21276062), the Natural Science Foundation of Hebei Province (B2010000035, B2011202095), the Science and Technology Research Key Project of Higher School in Hebei Province (ZD2010118)

Immobilization of Papain in Biosilica Matrix and Its Catalytic Property

ZHOU Liya¹, WANG Cui¹, JIANG Yanjun^1,2, GAO Jing¹

1 School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130, China;
2 National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China

Received:2012-06-25 Revised:2012-10-20 Online:2013-06-28 Published:2013-07-03
Contact: GAO Jing
Supported by:
Supported by the National Natural Science Foundation of China (21006020, 21276060, 21276062), the Natural Science Foundation of Hebei Province (B2010000035, B2011202095), the Science and Technology Research Key Project of Higher School in Hebei Province (ZD2010118)

摘要/Abstract

摘要： A novel method was developed for papain immobilization through a biomimetic silicification process induced by papain. By incubating papain in a silica precursor solution, the papain-silica composite formed rapidly and papain was encapsulated. The encapsulation efficiency and the recovery activity were 82.60% and 83.09%, respectively. Compared with enzymes and biomolecules immobilized in biosilica matrix in the presence of additional silica-precipitating species, this papain encapsulation process, a biomimetic approach, realized high encapsulation efficiency by its autosilification activity under mild conditions (near-neutral pH and ambient temperature). Furthermore, the encapsulated papain exhibits enhanced thermal, pH, recycling and storage stabilities. Kinetic analysis showed that the biomimetic silica matrix did not significantly hinder the mass transport of substrate or the release of product.

关键词: papain, silica matrix, encapsulation, biosilicification, biomimetic

Abstract: A novel method was developed for papain immobilization through a biomimetic silicification process induced by papain. By incubating papain in a silica precursor solution, the papain-silica composite formed rapidly and papain was encapsulated. The encapsulation efficiency and the recovery activity were 82.60% and 83.09%, respectively. Compared with enzymes and biomolecules immobilized in biosilica matrix in the presence of additional silica-precipitating species, this papain encapsulation process, a biomimetic approach, realized high encapsulation efficiency by its autosilification activity under mild conditions (near-neutral pH and ambient temperature). Furthermore, the encapsulated papain exhibits enhanced thermal, pH, recycling and storage stabilities. Kinetic analysis showed that the biomimetic silica matrix did not significantly hinder the mass transport of substrate or the release of product.

Key words: papain, silica matrix, encapsulation, biosilicification, biomimetic

周丽亚, 王翠, 姜艳军, 高静. Immobilization of Papain in Biosilica Matrix and Its Catalytic Property[J]. Chinese Journal of Chemical Engineering, 2013, 21(6): 670-675.

ZHOU Liya, WANG Cui, JIANG Yanjun, GAO Jing . Immobilization of Papain in Biosilica Matrix and Its Catalytic Property[J]. Chin.J.Chem.Eng., 2013, 21(6): 670-675.

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Immobilization of Papain in Biosilica Matrix and Its Catalytic Property

Immobilization of Papain in Biosilica Matrix and Its Catalytic Property

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