Immobilization of trophic anaerobic acetogen for semi-continuous syngas fermentation

doi:10.1016/j.cjche.2020.07.041

中国化学工程学报 ›› 2021, Vol. 29 ›› Issue (1): 311-316.DOI: 10.1016/j.cjche.2020.07.041

• Special Topic: Biocatalysis • 上一篇下一篇

Immobilization of trophic anaerobic acetogen for semi-continuous syngas fermentation

Lijuan Zhang¹, Peng Hu², Jiang Pan¹, Huilei Yu¹, Jianhe Xu¹

1 State Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Centre for Biomanufacturing, East China University of Science and Technology, Shanghai 200237, China;
2 Shanghai GTL Biotech Co. Ltd., Shanghai 200438, China

收稿日期:2020-03-29 修回日期:2020-06-25 出版日期:2021-01-28 发布日期:2021-04-02
通讯作者: Jiang Pan, Huilei Yu
基金资助:
This work was supported by the National Key Research and Development Program of China (2019YFA0905000), the National Natural Science Foundation of China (21536004, 21922804, 21776085, and 21871085) and the Fundamental Research Funds for the Central Universities (22221818014).

Immobilization of trophic anaerobic acetogen for semi-continuous syngas fermentation

Lijuan Zhang¹, Peng Hu², Jiang Pan¹, Huilei Yu¹, Jianhe Xu¹

1 State Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Centre for Biomanufacturing, East China University of Science and Technology, Shanghai 200237, China;
2 Shanghai GTL Biotech Co. Ltd., Shanghai 200438, China

Received:2020-03-29 Revised:2020-06-25 Online:2021-01-28 Published:2021-04-02
Contact: Jiang Pan, Huilei Yu
Supported by:
This work was supported by the National Key Research and Development Program of China (2019YFA0905000), the National Natural Science Foundation of China (21536004, 21922804, 21776085, and 21871085) and the Fundamental Research Funds for the Central Universities (22221818014).

摘要/Abstract

摘要： The massive consumption of fossil energy forces people to find new sources of energy. Syngas fermentation has become a hot research field as its high potential in renewable energy production and sustainable development. In this study, trophic anaerobic acetogen Morella thermoacetica was successfully immobilized by calcium alginate embedding method. The ability of the immobilized cells on production of acetic acid through syngas fermentation was compared in both airlift and bubble column bioreactors. The bubble column bioreactor was selected as the better type of bioreactor. The production of acetic acid reached 32.3 g·L^-1 in bubble column bioreactor with a space-time yield of 2.13 g·L^-1·d^-1. The immobilized acetogen could be efficiently reused without significant lag period, even if exposed to air for a short time. A semi-continuous syngas fermentation was performed using immobilized cells, with an average space-time acetic acid yield of 3.20 g·L^-1·d^-1. After 30 days of fermentation, no significant decrease of the acetic acid production rate was observed.

关键词: Anaerobic, Syngas, Acetic acid, Immobilization, Bioreactors, Semi-continuous fermentation

Abstract: The massive consumption of fossil energy forces people to find new sources of energy. Syngas fermentation has become a hot research field as its high potential in renewable energy production and sustainable development. In this study, trophic anaerobic acetogen Morella thermoacetica was successfully immobilized by calcium alginate embedding method. The ability of the immobilized cells on production of acetic acid through syngas fermentation was compared in both airlift and bubble column bioreactors. The bubble column bioreactor was selected as the better type of bioreactor. The production of acetic acid reached 32.3 g·L^-1 in bubble column bioreactor with a space-time yield of 2.13 g·L^-1·d^-1. The immobilized acetogen could be efficiently reused without significant lag period, even if exposed to air for a short time. A semi-continuous syngas fermentation was performed using immobilized cells, with an average space-time acetic acid yield of 3.20 g·L^-1·d^-1. After 30 days of fermentation, no significant decrease of the acetic acid production rate was observed.

Key words: Anaerobic, Syngas, Acetic acid, Immobilization, Bioreactors, Semi-continuous fermentation

Lijuan Zhang, Peng Hu, Jiang Pan, Huilei Yu, Jianhe Xu. Immobilization of trophic anaerobic acetogen for semi-continuous syngas fermentation[J]. 中国化学工程学报, 2021, 29(1): 311-316.

Lijuan Zhang, Peng Hu, Jiang Pan, Huilei Yu, Jianhe Xu. Immobilization of trophic anaerobic acetogen for semi-continuous syngas fermentation[J]. Chinese Journal of Chemical Engineering, 2021, 29(1): 311-316.

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Immobilization of trophic anaerobic acetogen for semi-continuous syngas fermentation

Immobilization of trophic anaerobic acetogen for semi-continuous syngas fermentation

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