SCI和EI收录∣中国化工学会会刊

中国化学工程学报 ›› 2023, Vol. 53 ›› Issue (1): 324-331.DOI: 10.1016/j.cjche.2022.01.022

• Full Length Article • 上一篇    下一篇

Production of palmitoleic acid by oleaginous yeast Scheffersomyces segobiensis DSM 27193 using systematic dissolved oxygen regulation strategy

Xinhai Zhou1, Dawei Zhou1, Xinhui Bao1, Yang Zhang2, Jie Zhou1, Fengxue Xin1,3, Wenming Zhang1,3, Xiujuan Qian1, Weiliang Dong1,3, Min Jiang1,3, Katrin Ochsenreither4   

  1. 1. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, China;
    2. Institute of Microbiology, Chinese Academy of Science, Beijing 100101, China;
    3. Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University, Nanjing 211800, China;
    4. Institute of Process Engineering in Life Sciences, Section II: Technical Biology, Karlsruhe Institute of Technology, Fritz-Haber-Weg4, Karlsruhe 76131, Germany
  • 收稿日期:2021-08-20 修回日期:2022-01-18 出版日期:2023-01-28 发布日期:2023-04-08
  • 通讯作者: Xiujuan Qian,E-mail:xiujuanqian@njtech.edu.cn;Weiliang Dong,E-mail:dwl@njtech.edu.cn
  • 基金资助:
    This work was supported by the National Key Research & Development Program of China (2021YFC2101500, 2018YFA0902200), National Natural Science Foundation of China (22008115, 21978130), Jiangsu Province Natural Science Foundation for Youths (SBK2020044721), Jiangsu Provincial Agricultural Science and Technology Independent Innovation Fund Project (CX(21)3120), Jiangsu Planned Projects for Postdoctoral Research Funds (2021K085A), China Postdoctoral Science Foundation (2020M671467), and Postdoctoral Research Funding Program of Jiangsu Province (2021K085A).

Production of palmitoleic acid by oleaginous yeast Scheffersomyces segobiensis DSM 27193 using systematic dissolved oxygen regulation strategy

Xinhai Zhou1, Dawei Zhou1, Xinhui Bao1, Yang Zhang2, Jie Zhou1, Fengxue Xin1,3, Wenming Zhang1,3, Xiujuan Qian1, Weiliang Dong1,3, Min Jiang1,3, Katrin Ochsenreither4   

  1. 1. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, China;
    2. Institute of Microbiology, Chinese Academy of Science, Beijing 100101, China;
    3. Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University, Nanjing 211800, China;
    4. Institute of Process Engineering in Life Sciences, Section II: Technical Biology, Karlsruhe Institute of Technology, Fritz-Haber-Weg4, Karlsruhe 76131, Germany
  • Received:2021-08-20 Revised:2022-01-18 Online:2023-01-28 Published:2023-04-08
  • Contact: Xiujuan Qian,E-mail:xiujuanqian@njtech.edu.cn;Weiliang Dong,E-mail:dwl@njtech.edu.cn
  • Supported by:
    This work was supported by the National Key Research & Development Program of China (2021YFC2101500, 2018YFA0902200), National Natural Science Foundation of China (22008115, 21978130), Jiangsu Province Natural Science Foundation for Youths (SBK2020044721), Jiangsu Provincial Agricultural Science and Technology Independent Innovation Fund Project (CX(21)3120), Jiangsu Planned Projects for Postdoctoral Research Funds (2021K085A), China Postdoctoral Science Foundation (2020M671467), and Postdoctoral Research Funding Program of Jiangsu Province (2021K085A).

摘要: Palmitoleic acid (POA) can be naturally found only in few oil seeds and has significant applications in pharmaceutical industry. Recently, the isolated oleaginous yeast Scheffersomyces segobiensis DSM 27193 was identified with high content of POA in its intracellular lipid (13.80%). In this study, process optimization focused on dissolved oxygen regulation to improve POA production was conducted. Dynamic agitation was found to do significant enhancement on POA-rich lipid production than aeration regulation. Under the best condition of 1000 r·min-1 of agitation and 1 vvm (airvolume/culture volume/min) of aeration, no ethanol was detected during the whole fermentation process, while a dry biomass concentration of 44.80 g·L-1 with 13.43 g·L-1 of lipid and 2.93 g·L-1 of POA was achieved. Transcription analysis revealed that the ethanol synthetic pathway was downregulated under the condition of high agitation, while the expression of the key enzymes responsible for lipid and POA accumulation were enhanced.

关键词: Palmitoleic acid production, Scheffersomyces segobiensis, Dissolved oxygen, Ethanol, Bioprocess, Bioreactors

Abstract: Palmitoleic acid (POA) can be naturally found only in few oil seeds and has significant applications in pharmaceutical industry. Recently, the isolated oleaginous yeast Scheffersomyces segobiensis DSM 27193 was identified with high content of POA in its intracellular lipid (13.80%). In this study, process optimization focused on dissolved oxygen regulation to improve POA production was conducted. Dynamic agitation was found to do significant enhancement on POA-rich lipid production than aeration regulation. Under the best condition of 1000 r·min-1 of agitation and 1 vvm (airvolume/culture volume/min) of aeration, no ethanol was detected during the whole fermentation process, while a dry biomass concentration of 44.80 g·L-1 with 13.43 g·L-1 of lipid and 2.93 g·L-1 of POA was achieved. Transcription analysis revealed that the ethanol synthetic pathway was downregulated under the condition of high agitation, while the expression of the key enzymes responsible for lipid and POA accumulation were enhanced.

Key words: Palmitoleic acid production, Scheffersomyces segobiensis, Dissolved oxygen, Ethanol, Bioprocess, Bioreactors