SCI和EI收录∣中国化工学会会刊

中国化学工程学报 ›› 2021, Vol. 29 ›› Issue (1): 317-325.DOI: 10.1016/j.cjche.2020.07.042

• Special Topic: Biocatalysis • 上一篇    下一篇

An enzyme-loaded reactor using metal-organic framework-templated polydopamine microcapsule

Jing Wang, Yongqin Lv   

  1. Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
  • 收稿日期:2020-02-06 修回日期:2020-06-29 出版日期:2021-01-28 发布日期:2021-04-02
  • 通讯作者: Yongqin Lv
  • 基金资助:
    This work was supported by the National Natural Science Foundation of China (31961133004, 21861132017), the National Key Research and Development Program of China (2018YFA0902200), and the Fundamental Research Funds for the Central Universities (PT1917, buctrc201).

An enzyme-loaded reactor using metal-organic framework-templated polydopamine microcapsule

Jing Wang, Yongqin Lv   

  1. Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
  • Received:2020-02-06 Revised:2020-06-29 Online:2021-01-28 Published:2021-04-02
  • Contact: Yongqin Lv
  • Supported by:
    This work was supported by the National Natural Science Foundation of China (31961133004, 21861132017), the National Key Research and Development Program of China (2018YFA0902200), and the Fundamental Research Funds for the Central Universities (PT1917, buctrc201).

摘要: Ultrathin polydopamine microcapsules with hierarchical structure and porosity were prepared for the immobilization of multienzymes using metal-organic framework (MOF) as the template. The multienzyme/MOF composite was first prepared using a “one-pot” co-precipitation approach via the coordination and self-assembly of zinc ions and 2-methylimidazole in the presence of enzymes. The obtained nanoparticles were then coated with polydopamine thin layer through the self-polymerization of dopamine under alkaline condition. The polydopamine microcapsules with an ultrathin shell thickness of ~48 nm were finally generated by removing the MOF template at acidic condition. Three enzymes were encapsulated in PDA microcapsules including carbonic anhydrase (CA), formate dehydrogenase (FateDH), and glutamate dehydrogenase (GDH). FateDH that catalyzed the main reaction of CO2 reduction to formic acid retained 94.7% activity of equivalent free FateDH. Compared with free multienzymes, the immobilized ones embedded in PDA microcapsules exhibited 4.5-times higher of formate production and high catalytic efficiency with a co-factor-based formate yield of 342%.

关键词: Biocatalysis, Enzyme, Immobilization, Microcapsule, Metal–organic framework, Template

Abstract: Ultrathin polydopamine microcapsules with hierarchical structure and porosity were prepared for the immobilization of multienzymes using metal-organic framework (MOF) as the template. The multienzyme/MOF composite was first prepared using a “one-pot” co-precipitation approach via the coordination and self-assembly of zinc ions and 2-methylimidazole in the presence of enzymes. The obtained nanoparticles were then coated with polydopamine thin layer through the self-polymerization of dopamine under alkaline condition. The polydopamine microcapsules with an ultrathin shell thickness of ~48 nm were finally generated by removing the MOF template at acidic condition. Three enzymes were encapsulated in PDA microcapsules including carbonic anhydrase (CA), formate dehydrogenase (FateDH), and glutamate dehydrogenase (GDH). FateDH that catalyzed the main reaction of CO2 reduction to formic acid retained 94.7% activity of equivalent free FateDH. Compared with free multienzymes, the immobilized ones embedded in PDA microcapsules exhibited 4.5-times higher of formate production and high catalytic efficiency with a co-factor-based formate yield of 342%.

Key words: Biocatalysis, Enzyme, Immobilization, Microcapsule, Metal–organic framework, Template