Aggregation-regulated bioreduction process of graphene oxide by Shewanella bacteria

doi:10.1016/j.cjche.2024.01.008

中国化学工程学报 ›› 2024, Vol. 69 ›› Issue (5): 56-62.DOI: 10.1016/j.cjche.2024.01.008

Aggregation-regulated bioreduction process of graphene oxide by Shewanella bacteria

Kaixin Han¹, Yibo Zeng², Yinghua Lu¹, Ping Zeng³, Liang Shen¹

1. Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China;
2. Pen-Tung Sah Institute of Micro-Nano Science and Technology, Xiamen University, Xiamen 361005, China;
3. Chinese Research Academy of Environmental Sciences, Beijing 100012, China

收稿日期:2023-09-26 修回日期:2023-12-30 出版日期:2024-05-28 发布日期:2024-07-01
通讯作者: Ping Zeng,E-mail:zengping@craes.org.cn;Liang Shen,E-mail:shenliang@xmu.edu.cn
基金资助:
This work was supported by the National Natural Science Foundation of China (22178293) and the Natural Science Foundation of Fujian Province of China (2022J01022).

Aggregation-regulated bioreduction process of graphene oxide by Shewanella bacteria

Kaixin Han¹, Yibo Zeng², Yinghua Lu¹, Ping Zeng³, Liang Shen¹

1. Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China;
2. Pen-Tung Sah Institute of Micro-Nano Science and Technology, Xiamen University, Xiamen 361005, China;
3. Chinese Research Academy of Environmental Sciences, Beijing 100012, China

Received:2023-09-26 Revised:2023-12-30 Online:2024-05-28 Published:2024-07-01
Contact: Ping Zeng,E-mail:zengping@craes.org.cn;Liang Shen,E-mail:shenliang@xmu.edu.cn
Supported by:
This work was supported by the National Natural Science Foundation of China (22178293) and the Natural Science Foundation of Fujian Province of China (2022J01022).

摘要/Abstract

摘要： The bioreduction of graphene oxide (GO) using environmentally functional bacteria such as Shewanella represents a green approach to produce reduced graphene oxide (rGO). This process differs from the chemical reduction that involves instantaneous molecular reactions. In bioreduction, the contact of bacterial cells and GO is considered the rate-limiting step. To reveal how the bacteria-GO integration regulates rGO production, the comparative experiments of GO and three Shewanella strains were carried out. Fourier-transform infrared spectroscopy, X-ray photoelectron spectroscopy, Raman spectroscopy, and atomic force microscopy were used to characterize the reduction degree and the aggregation degree. The results showed that a spontaneous aggregation of GO and Shewanella into the condensed entity occurred within 36 h. A positive linear correlation was established, linking three indexes of the aggregation potential, the bacterial reduction ability, and the reduction degree (I_D/I_G) comprehensively.

关键词: Graphene oxide, Reduced graphene oxide, Bioreduction, Aggregation, Shewanella

Abstract: The bioreduction of graphene oxide (GO) using environmentally functional bacteria such as Shewanella represents a green approach to produce reduced graphene oxide (rGO). This process differs from the chemical reduction that involves instantaneous molecular reactions. In bioreduction, the contact of bacterial cells and GO is considered the rate-limiting step. To reveal how the bacteria-GO integration regulates rGO production, the comparative experiments of GO and three Shewanella strains were carried out. Fourier-transform infrared spectroscopy, X-ray photoelectron spectroscopy, Raman spectroscopy, and atomic force microscopy were used to characterize the reduction degree and the aggregation degree. The results showed that a spontaneous aggregation of GO and Shewanella into the condensed entity occurred within 36 h. A positive linear correlation was established, linking three indexes of the aggregation potential, the bacterial reduction ability, and the reduction degree (I_D/I_G) comprehensively.

Key words: Graphene oxide, Reduced graphene oxide, Bioreduction, Aggregation, Shewanella

Kaixin Han, Yibo Zeng, Yinghua Lu, Ping Zeng, Liang Shen. Aggregation-regulated bioreduction process of graphene oxide by Shewanella bacteria[J]. 中国化学工程学报, 2024, 69(5): 56-62.

Kaixin Han, Yibo Zeng, Yinghua Lu, Ping Zeng, Liang Shen. Aggregation-regulated bioreduction process of graphene oxide by Shewanella bacteria[J]. Chinese Journal of Chemical Engineering, 2024, 69(5): 56-62.

参考文献

[1] H.J. Shin, K.K. Kim, A. Benayad, S.M. Yoon, H.K. Park, I.S. Jung, M.H. Jin, H.K. Jeong, J.M. Kim, J.Y. Choi, Y.H. Lee, Efficient reduction of graphite oxide by sodium borohydride and its effect on electrical conductance, Adv. Funct. Mater. 19(12)(2009)1987-1992.
[2] S. Stankovich, D.A. Dikin, R.D. Piner, K.A. Kohlhaas, A. Kleinhammes, Y.Y. Jia, Y. Wu, S.T. Nguyen, R.S. Ruoff, Synthesis of graphene-based nanosheets via chemical reduction of exfoliated graphite oxide, Carbon 45(7)(2007)1558-1565.
[3] J. Wu, H. Lin, D.J. Moss, K.P. Loh, B. Jia, Graphene oxide for photonics, electronics and optoelectronics, Nat Rev Chem 7(3)(2023)162-183.
[4] V. Agarwal, P.B. Zetterlund, Strategies for reduction of graphene oxide-A comprehensive review, Chem. Eng. J. 405(2021)127018.
[5] L. Shen, Z.H. Jin, D. Wang, Y.P. Wang, Y.H. Lu, Enhance wastewater biological treatment through the bacteria induced graphene oxide hydrogel, Chemosphere 190(2018)201-210.
[6] G.M. Wang, F. Qian, C.W. Saltikov, Y.Q. Jiao, Y. Li, Microbial reduction of graphene oxide by Shewanella, Nano Res. 4(6)(2011)563-570.
[7] S. Gurunathan, J.W. Han, V. Eppakayala, J.H. Kim, Microbial reduction of graphene oxide by Escherichia coli:A green chemistry approach, Colloids Surf. B Biointerfaces 102(2013)772-777.
[8] O. Akhavan, E. Ghaderi, Escherichia coli bacteria reduce graphene oxide to bactericidal graphene in a self-limiting manner, Carbon 50(5)(2012)1853-1860.
[9] Q. Xu, X. Lin, L. Gan, G. Owens, Z. Chen, Green reduction of graphene oxide using Bacillus sphaericus, J. Colloid Interface Sci. 605(2022)881-887.
[10] Y. Lu, L.R. Zhong, L. Tang, H. Wang, Z.H. Yang, Q.Q. Xie, H.P. Feng, M.Y. Jia, C.Z. Fan, Extracellular electron transfer leading to the biological mediated production of reduced graphene oxide, Chemosphere 256(2020)127141.
[11] B.H. Xu, S.H. Cheng, M.Y. Han, Q. Li, W.F. Chen, W.Z. Zhou, The characteristic and performance of reduced graphene oxide by marine bacterium Pseudoalteromonas sp. CF10-13, Ceram. Int. 46(13)(2020)21699-21706.
[12] D. Wang, Z.H. Jin, X. Pang, X. Jiang, Y.H. Lu, L. Shen, Fabrication and functionalization of biological graphene aerogel by reusing microorganism in activated sludge and ionic dyes, Chem. Eng. J. 392(2020)124823.
[13] L. Shi, H.L. Dong, G. Reguera, H. Beyenal, A.H. Lu, J. Liu, H.Q. Yu, J.K. Fredrickson, Extracellular electron transfer mechanisms between microorganisms and minerals, Nat. Rev. Microbiol. 14(10)(2016)651-662.
[14] E.C. Salas, Z.Z. Sun, A. Luttge, J.M. Tour, Reduction of graphene oxide via bacterial respiration, ACS Nano 4(8)(2010)4852-4856.
[15] B. Schuetz, M. Schicklberger, J. Kuermann, A. Spormann, J. Gescher, Periplasmic electron transfer via the c-Type cytochromes MtrA and FccA of Shewanella oneidensis MR-1, Appl Environ Microbiol, 75(24)(2009)7789-7796.
[16] L. Zou, F. Zhu, Z.E. Long, Y.H. Huang, Bacterial extracellular electron transfer:A powerful route to the green biosynthesis of inorganic nanomaterials for multifunctional applications, J. Nanobiotechnology 19(1)(2021)120.
[17] C. Vargas, R. Simarro, J.A. Reina, L.F. Bautista, N. Gonzalez-Benitez, New approach for biological synthesis of reduced graphene oxide, Biochem Eng J. 151(2019):107331.
[18] W.H. Xu, Z.H. Jin, X. Pang, Y.B. Zeng, X. Jiang, Y.H. Lu, L. Shen, Interaction between biocompatible graphene oxide and live Shewanella in the self-assembled hydrogel:The role of physicochemical properties, ACS Appl. Bio Mater. 3(7)(2020)4263-4272.
[19] J. Ming, D. Sun, J.P. Wei, X.L. Chen, N.F. Zheng, Adhesion of bacteria to a graphene oxide film, ACS Appl. Bio Mater. 3(1)(2020)704-712.
[20] D.C. Marcano, D.V. Kosynkin, J.M. Berlin, A. Sinitskii, Z.Z. Sun, A. Slesarev, L.B. Alemany, W. Lu, J.M. Tour, Improved synthesis of graphene oxide, ACS Nano 4(8)(2010)4806-4814.
[21] H.J. Butt, B. Cappella, M. Kappl, Force measurements with the atomic force microscope:Technique, interpretation and applications, Surf. Sci. Rep. 59(1-6)(2005)1-152.
[22] F. Alam, S. Kumar, K.M. Varadarajan, Quantification of adhesion force of bacteria on the surface of biomaterials:Techniques and assays, ACS Biomater. Sci. Eng. 5(5)(2019)2093-2110.
[23] K.X. Han, Y.B. Zeng, Y.H. Lu, S.J. Meng, Y.Z. Hong, L. Shen, Mechanistic insights into aggregation process of graphene oxide and bacterial cells in microbial reduction of ferrihydrite, Sci. Total Environ. 857(Pt 1)(2023)159321.
[24] L. Shi, T.C. Squier, J.M. Zachara, J.K. Fredrickson, Respiration of metal (hydr) oxides by Shewanella and Geobacter:A key role for multihaem c-type cytochromes, Mol. Microbiol. 65(1)(2007)12-20.
[25] E.J. O'Loughlin, Effects of electron transfer mediators on the bioreduction of lepidocrocite (gamma-FeOOH) by Shewanella putrefaciens CN₃ 2, Environ. Sci. Technol. 42(18)(2008)6876-6882.
[26] J. Huang, A. Jones, T.D. Waite, Y. Chen, H. Zhang, Fe (II) redox chemistry in the environment, Chem Rev. 121(13)(2021)8161-8233.
[27] S. Raveendran, N. Chauhan, Y. Nakajima, H. Toshiaki, S. Kurosu, Y. Tanizawa, R. Tero, Y. Yoshida, T. Hanajiri, T. Maekawa, P.M. Ajayan, A. Sandhu, D.S. Kumar, Ecofriendly route for the synthesis of highly conductive graphene using extremophiles for green electronics and bioscience, Part. Part. Syst. Charact. 30(7)(2013)573-578.
[28] L. Liu, C.L. Zhu, M.M. Fan, C.T. Chen, Y. Huang, Q.L. Hao, J.Z. Yang, H.Y. Wang, D.P. Sun, Oxidation and degradation of graphitic materials by naphthalene-degrading bacteria, Nanoscale 7(32)(2015)13619-13628.
[29] R. Sharma, J.H. Baik, C.J. Perera, M.S. Strano, Anomalously large reactivity of single graphene layers and edges toward electron transfer chemistries, Nano Lett. 10(2)(2010)398-405.
[30] X.L. Lu, X.D. Feng, J.R. Werber, C.H. Chu, I. Zucker, J.H. Kim, C.O. Osuji, M. Elimelech, Enhanced antibacterial activity through the controlled alignment of graphene oxide nanosheets, Proc. Natl. Acad. Sci. USA 114(46)(2017) E9793-E9801.
[31] F. Perreault, A.F. de Faria, S. Nejati, M. Elimelech, Antimicrobial properties of graphene oxide nanosheets:Why size matters, ACS Nano 9(7)(2015)7226-7236.
[32] J. Chen, Y. Zhang, M. Zhang, B.W. Yao, Y.R. Li, L. Huang, C. Li, G.Q. Shi, Water-enhanced oxidation of graphite to graphene oxide with controlled species of oxygenated groups, Chem. Sci. 7(3)(2016)1874-1881.

Aggregation-regulated bioreduction process of graphene oxide by Shewanella bacteria

Aggregation-regulated bioreduction process of graphene oxide by Shewanella bacteria

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