Optimization of DsbA Purification from Recombinant Escherichia coli Broth Using Box-Behnken Design Methodology

doi:10.1016/S1004-9541(13)60457-7

Chinese Journal of Chemical Engineering ›› 2013, Vol. 21 ›› Issue (2): 185-191.DOI: 10.1016/S1004-9541(13)60457-7

Optimization of DsbA Purification from Recombinant Escherichia coli Broth Using Box-Behnken Design Methodology

罗曼, 关怡新, 姚善泾

Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China

收稿日期:2011-05-13 修回日期:2011-10-16 出版日期:2013-02-28 发布日期:2013-03-13
通讯作者: GUAN Yixin
基金资助:
Supported by the National Natural Science Foundation of China (21036005).

Optimization of DsbA Purification from Recombinant Escherichia coli Broth Using Box-Behnken Design Methodology

LUO Man, GUAN Yixin, YAO Shanjing

Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China

Received:2011-05-13 Revised:2011-10-16 Online:2013-02-28 Published:2013-03-13

摘要/Abstract

摘要： Disulfide bond formation protein A (DsbA) is one of the important helper proteins for folding in protein synthesis in vivo. In this study, purification of recombinant DsbA was investigated by examining four important factors with Box-Behnken design method, a statistic-based design of experiments. The optimal operation conditions were obtained by adopting the effectiveness coefficient method on the multi-objective problem, which takes the protein recovery, purification efficiency and throughput of ion-exchange chromatography into account. After the optimization, protein recovery of 96.8% and purity higher than 95% DsbA was achieved, and the productivity was (377.9?1.7) mg soluble DsbA per liter broth. The purified protein was identified by peptide mass fingerprinting matching the record of gi|2624856, a mutant of DsbA. The DsbA was preliminarily applied to the refolding of denatured lysozyme in vitro.

关键词: disulfide bond formation protein A, protein purification, Box-Behnken experiment design, response surface methodology, multi-object programming

Abstract: Disulfide bond formation protein A (DsbA) is one of the important helper proteins for folding in protein synthesis in vivo. In this study, purification of recombinant DsbA was investigated by examining four important factors with Box-Behnken design method, a statistic-based design of experiments. The optimal operation conditions were obtained by adopting the effectiveness coefficient method on the multi-objective problem, which takes the protein recovery, purification efficiency and throughput of ion-exchange chromatography into account. After the optimization, protein recovery of 96.8% and purity higher than 95% DsbA was achieved, and the productivity was (377.9?1.7) mg soluble DsbA per liter broth. The purified protein was identified by peptide mass fingerprinting matching the record of gi|2624856, a mutant of DsbA. The DsbA was preliminarily applied to the refolding of denatured lysozyme in vitro.

Key words: disulfide bond formation protein A, protein purification, Box-Behnken experiment design, response surface methodology, multi-object programming

罗曼, 关怡新, 姚善泾. Optimization of DsbA Purification from Recombinant Escherichia coli Broth Using Box-Behnken Design Methodology[J]. Chinese Journal of Chemical Engineering, 2013, 21(2): 185-191.

LUO Man, GUAN Yixin, YAO Shanjing. Optimization of DsbA Purification from Recombinant Escherichia coli Broth Using Box-Behnken Design Methodology[J]. Chin.J.Chem.Eng., 2013, 21(2): 185-191.

参考文献

1 Bardwell, J.C.A., McGovern, K., Beckwith, J., “Identification of a protein required for disulfide bond formation in vivo”, Cell, 67, 581-589 (1991).

2 Kamitani, S., Akiyama, Y., Ito, K., “Identification and characterization of an Escherichia coli gene required for the formation of correctly folded alkaline phosphatase, a periplasmic enzyme”, EMBO J, 11, 57-62 (1992).

3 Plunkett, G., Burland, V., Daniels, D.L., Blattner, F.R., “Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes”, Nucleic Acids Res., 21, 3391-3398 (1993).

4 Wunderlich, M., Jaenicke, R., Glockshuber, R., “The redox properties of protein disulfide isomerase (DsbA) of Eshcherichia coli result from a tense conformation of its oxidized form”, J. Mol. Biol., 233, 559-566 (1993).

5 Zheng, W.D., Quan, H., Song, J.L., Yang, S.L., Wang, C.C., “Does DsbA have chaperone-like activity?”, Arch. Biochem. Biophys., 337, 326-331 (1997).

6 Debarbieux, L., Beckwith, J., “Electron avenue: Pathways of disulfide bond formation and isomerization”, Cell, 99, 117-119 (1999).

7 Collet, J.F., Bardwell, J.C.A., “Oxidative protein folding in bacteria”, Mol. Microbiol., 44, 1-8 (2002).

8 Box, G., Behnken, D., “Some new three-level designs for the study of quantitative variables”, Technometrics, 2, 455-475 (1960).

9 Ferreira, S.L.C., Bruns, R.E., Ferreira, H.S., Matos, G.D., David, J.M., Brandao, G.C., da Silva, E.G.P., Portugal, L.A., dos Reis, P.S., Souza, A.S., dos Santos, W.N.L., “Box-Behnken design: An alternative for the optimization of analytical methods”, Anal. Chim. Acta, 597, 179-186 (2007).

10 Sun, M., “Some issues in measuring and reporting solution quality of interactive multiple objective programming procedures”, Eur. J. Oper. Res., 162, 468-483 (2005).

11 Tozer, P.R., Stokes, J.R. “A multi-objective programming approach to feed ration balancing and nutrient management”, Agr. Syst., 67, 205-215 (2001).

12 Sun, M., “A multiple objective programming approach for determining faculty salary equity adjustments”, Eur. J. Oper. Res., 138, 302-319 (2002).

13 Pappin, D.J.C., Hojrup, P., Bleasby, A.J., “Rapid identification of proteins by peptide-mass fingerprinting”, Curr. Biol., 3, 327-332 (1993).

14 Mann, M., Højrup, P., Roepstorff, P., “Use of mass spectrometric molecular weight information to identify proteins in sequence databases”, Biol. Mass Spectrom., 22, 338-345 (1993).

15 Henzela, W.J., Watanabea, C., Stults, J.T., “Protein identification: the origins of peptide mass fingerprinting”, J. Am. Soc. Mass Spectr., 14, 931-942 (2003).

16 Luo, M., Guan, Y.X., Yao, S.J., “Statistical optimization of protein disulfide isomerase expression in E. coli”, Chin. J. Biochem. Mol. Biol., 21, 376-383 (2005).

17 Zhang, C., Fan, D.D., Shang, L.A., Ma, X.X., Luo, Y.E., Xue, W.J., Gao, P.F., “Optimization of fermentation process for human-like collagen production of recombinant Escherichia coli using response surface methodology”, Chin. J. Chem. Eng., 18, 137-142 (2010).

18 Bradford, M.M., “A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding”, Anal. Biochem., 72, 248-254 (1976).

19 Stellmach, B., Qian, J.Y., Enzyme Measurement, China Light Industry Press, Beijing (1992). (in Chinese)

20 Luo, M., Guan, Y.X., Yao, S.J., “Study on disulfide bond formation protein A in Escherichia coli”, Chin. J. Biotech., 23 (1), 7-15 (2007).

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Optimization of DsbA Purification from Recombinant Escherichia coli Broth Using Box-Behnken Design Methodology

Optimization of DsbA Purification from Recombinant Escherichia coli Broth Using Box-Behnken Design Methodology

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