Growth and Physiological Features of Cyanobacterium Anabaena sp. Strain PCC 7120 in a Glucose-Mixotrophic Culture

›› 2011, Vol. 19 ›› Issue (1): 108-115.

Growth and Physiological Features of Cyanobacterium Anabaena sp. Strain PCC 7120 in a Glucose-Mixotrophic Culture

喻国策^1,2, 施定基³, 蔡昭铃², 丛威², 欧阳藩²

1. Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing 100084, China;
2. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China;
3. Research Center of Photosynthesis, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China

收稿日期:2010-04-06 修回日期:2010-04-06 出版日期:2011-02-28 发布日期:2011-02-28
通讯作者: YU Guoce, E-mail: yugc@tsinghua.edu.cn
基金资助:
Supported by a grant from the State Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Chinese Academy of Sciences

Growth and Physiological Features of Cyanobacterium Anabaena sp. Strain PCC 7120 in a Glucose-Mixotrophic Culture

YU Guoce^1,2, SHI Dingji³, CAI Zhaoling², CONG Wei², OUYANG Fan²

1. Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing 100084, China;
2. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China;
3. Research Center of Photosynthesis, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China

Received:2010-04-06 Revised:2010-04-06 Online:2011-02-28 Published:2011-02-28
Supported by:
Supported by a grant from the State Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Chinese Academy of Sciences

摘要/Abstract

摘要： Mixotrophic growth is one potential mode for mass culture of microalgae and cyanobacteria particularly suitable for the production of high value bioactive compounds and fine chemicals.The typical heterocystous cyanobacterium Anabaena sp.PCC 7120 was grown in the presence of exogenous glucose in light.Glucose improved the cell growth evidently,the maximal specific growth rate under mixotrophic condition(0.38 d^-1)being 1.6-fold of that of photoautotrophic growth.Mixotrophy caused a variation in cellular pigment composition,increasing the content of chlorophyll a and decreasing the contents of carotenoid and phycobiliprotein relative to chlorophyll a.Fluorescence emission from photosystem Ⅱ(PSⅡ)relative to photosystem I was enhanced in mixotrophic cells,implying an increased energy distribution in PSⅡ.Glucokinase(EC 2.7.1.2)activity was further induced in the presence of glucose.The mixotrophic culture was scaled up in a 15 L airlift photobioreactor equipped with an inner and an outer light source.A modified Monod model incorporating the specific growth rate and the average light intensity in the reactor was developed to describe cell growth appropriately.The understanding of mixotrophic growth and relevant physiological features of Anabaena sp.PCC 7120 would be meaningful for cultivation and exploitation of this important cyanobacterial strain.

关键词: cyanobacteria, Anabaena sp.PCC 7120, growth features, mixotrophic culture, photobioreactor

Abstract: Mixotrophic growth is one potential mode for mass culture of microalgae and cyanobacteria particularly suitable for the production of high value bioactive compounds and fine chemicals.The typical heterocystous cyanobacterium Anabaena sp.PCC 7120 was grown in the presence of exogenous glucose in light.Glucose improved the cell growth evidently,the maximal specific growth rate under mixotrophic condition(0.38 d^-1)being 1.6-fold of that of photoautotrophic growth.Mixotrophy caused a variation in cellular pigment composition,increasing the content of chlorophyll a and decreasing the contents of carotenoid and phycobiliprotein relative to chlorophyll a.Fluorescence emission from photosystem Ⅱ(PSⅡ)relative to photosystem I was enhanced in mixotrophic cells,implying an increased energy distribution in PSⅡ.Glucokinase(EC 2.7.1.2)activity was further induced in the presence of glucose.The mixotrophic culture was scaled up in a 15 L airlift photobioreactor equipped with an inner and an outer light source.A modified Monod model incorporating the specific growth rate and the average light intensity in the reactor was developed to describe cell growth appropriately.The understanding of mixotrophic growth and relevant physiological features of Anabaena sp.PCC 7120 would be meaningful for cultivation and exploitation of this important cyanobacterial strain.

Key words: cyanobacteria, Anabaena sp.PCC 7120, growth features, mixotrophic culture, photobioreactor

喻国策, 施定基, 蔡昭铃, 丛威, 欧阳藩. Growth and Physiological Features of Cyanobacterium Anabaena sp. Strain PCC 7120 in a Glucose-Mixotrophic Culture[J]. , 2011, 19(1): 108-115.

YU Guoce, SHI Dingji, CAI Zhaoling, CONG Wei, OUYANG Fan. Growth and Physiological Features of Cyanobacterium Anabaena sp. Strain PCC 7120 in a Glucose-Mixotrophic Culture[J]. , 2011, 19(1): 108-115.

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Growth and Physiological Features of Cyanobacterium Anabaena sp. Strain PCC 7120 in a Glucose-Mixotrophic Culture

Growth and Physiological Features of Cyanobacterium Anabaena sp. Strain PCC 7120 in a Glucose-Mixotrophic Culture

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